Background and Aim: Human’s voice is observable and measurable like his other behaviours. Investigations have shown that characteristics of voice differ in males and females and possibly in different languages. Clinically, knowing natural voice characteristics is helpful for distinguishing voice pathologies. So the purpose of this study was to measure and compare the fundamental frequency human voice.

Materials and Methods: subjects were young males and females of Iranian Fars, Turk and Kurd races. First the subjects were asked to read some Persian sentences , then their voice samples were analysed by Dr.Speech software.

Results: Fundamental frequency mean is 168±46 Hz and 120±48 Hz for females and males , respectively , which shows significant difference. There is no significant difference between fundamental frequency mean of different races.

Conclusion: There is significant difference between fundamental frequency of males and females , but this difference is not attributable to their mother tongue. Key words: voice, fundamental frequency, Iranian, Fars , Turk , Kurd.