In this article we aimed at studying the function of Melanin in the inner ear. To reach this goal TTS was studied in people with blue or brown eye color. Subjects were 68 male teenagers with normal hearing. Auditory thresholds were obtained by means of high frequency audiometry (.8-8KHz)before and after TTS.  We used narrowband noise with frequency width of 1665-2335Hz, central frequency of 2 KHz, intensity of 105dBSPL and duration of 10 minutes. A significant difference was discovered in the average TTS of this two group in the frequency range of 2-8 KHz. Brown-colored eye boys manifested the least TTS and the blue- colored eyes one the most discovered TTS.