Iranian Journal of Diabetes and Lipid Disorders

EFFECT OF EIGHT-WEEK HIGH INTENSITY INTERVAL TRAINING AND RESVERATROL INTAKE ON SERUM ADIPONECTIN AND RESISTIN IN TYPE 2 DIABETIC RATS

Shahin Riyahi Malayeri

Background: Adiponectin and resin; Adipocytokine relateted with insulin resistance. The purpose of this study was to the Effect of eight-week high intensity interval training and resveratrol intake on Serum Adiponectin and resistin in type 2 diabetic rats.
Methods: During this experiment 32 diabetic rats with High-Dieting Diet Sprague Dowley race with an average weight of 250 ±10 grams and an average age of 10 weeks, were randomly divided into four groups, including the “control group”, the “supplement group”, the “training group” and the “training and supplement group”. For up to 8 weeks, the “practice” groups participated in 5 training sessions weekly; session was 18 minutes under the influence of High-Intensity Interval training with an intensity of 90 to %100 VO2max. moreover, the supplement groups were given 10 milligram daily doses of resveratrol intake solution per kilogram of body weight Gavage was given for 8 weeks and three times a week. The outcome data was analyzed using one-way analysis of variance and significance level of (P≤0.05).
Results: The results showed that eight weeks of exercise and resveratrol consumption increased on serum adiponectin, but did not have a statistically significant effect (P = 0.62). However, serum resistin decreased and significantly affected the training group (P = 0.001). Also, the mean values of fasting glucose, insulin and insulin resistance index decreased significantly in experimental groups (P = 0.001).
Conclusion: It seems high intensity interval training with resveratrol intake can lead to glucose control in type II diabetes by changing its insulin resistance factors.

THE EFFECT OF MUCILAGE EXTRACTED FROM THE FRUIT OF ABELMOSCHUS ESCULENTUS ON SERUM GLUCOSE AND LIPID LEVELS AND REORGANIZATION OF BETA CELLS IN DIABETIC RATS

Zahra Karampour Gebchag

Background: Due to the progress of diabetes and the use of alternative herbal medicines, In this study, the effects of oral administration of the mucilage extracted from pods of Abelmoschus esculentus (Ae) fruits on serum levels of glucose, lipids and morphology of Langerhans islets in diabetic rats was investigated.
Methods: In this experimental study, 24 female wistar rats were randomly allocated into four groups (n=6): normal control (NC), diabetic control (DC) and 2 diabetic groups that received (oral) 300 and 500 mg/kg/body weight of Abelmoschus esculentus. After preparing and confirming the type of, mucilage extraction from the fruit’s green okra was done by evaporation device in vacuum. Diabetes mellitus was induced by single dose intraperitoneal injection of streptozotocin 60mg/kg/body weight in diabetic groups. After 4 weeks, the serum levels of glucose and lipid profile of all groups were analyzed. Also morphology of Langerhans islets in the 4 groups was evaluated using H&E staining method. The data analyzed by SPSS software using ANOVA and Tukey tests.
Results: The results indicate a significant increase (P<0/05) in glucose, cholesterol, triglyceride, LDL and significant decrease (P<0.05) in HDL in diabetic rats compared to normal control. The use of the mucilage extracted from A. esculentus caused a significant decrease in serum levels of glucose, cholesterol, triglyceride, LDL and significant increase in serum level of HDL comparison with diabetic group.
Conclusion: according to the results of this study, the mucilage extracted from A. esculentus could be effective on control hyperlipidemia and hyperglycemia caused by diabetes mellitus.

BIOINFORMATIC STUDIES OF THE EFFECT OF THYMUS VULGARIS ON ALPHA-GLUCOSIDASE ENZYME INHIBITION FOR TREATING DIABETES

Azizeh Asadzadeh

Background: Inhibitors of α-glucosidase by interfering with digestion of carbohydrates play a role in controlling diabetes. Thymus vulgaris is an herb belonging to the mint family (lamiaceae). The essence of this plant contain the phenols such as thymol and carvacrol, cymene, linalool, pinene. The aim of this study was to investigate the inhibitory effect of the constituents of the thymus vulgaris extract on the activity of α-glucosidase enzyme by molecular docking.
Methods: In this study, to investigate how the compounds are attached to the active site of the enzyme, mapping of chemical structure of the compounds, energy optimization, docking studies and final analysis were carried out by ChemDraw, HyperChem, AutoDock 4.2, DS Visualizer and Lig Pluto software, respectively.
Results: All of the studied compounds were able to occupy the active site of the enzyme, among all of them, the best results of docking was related to the combination of Caryophlla-4- (12), 8 (13) -dien-5-β-ol. In fact, this compound has the most negative energy level of connection, the highest affinity for binding to the active site of the enzyme and the interaction site is similar to that of the co-crystal molecule.
Conclusion: considering the high efficacy of plant extract compounds in the bioinformatics study, and for complementary studies, the effect of the extract of this plant can be analyzed in order to control the increase of glucose in vitro and in vivo conditions.

THE EFFECT OF 12 WEEKS OF HIGH INTENSITY TRAINING ON IL-6, GLP-1 AND LIPID PROFILES IN TYPE 2 DIABETIC PATIENTS

Mohammad Marandi

Background: The aim of this study was to investigate the effect of 12 weeks of high intensity training on IL-
6, GLP-1 and lipid profiles in type 2 diabetic patients

Methods: 80 participants (40-50 years old) volunteered to participate in the study and divided into eight groups (four groups of severe periodic exercises and four uncontrolled control groups). Blood samples were collected and analyzed for measuring IL-6, GLP-1, lipid profile and glycemic index before and after weeks 4, 8 and after the training program.
Results: After 12 weeks of training, the effect of time and interaction between time × group on GLP-1 and IL-6 variables was statistically significant. Also, there was a significant difference between groups based on 12 weeks of training in these indices. Lipid profile was significantly improved in type 2 diabetic and glycemic control in obese and non-diabetic groups that had 12 weeks of high intensity interval training.

Conclusion: Overall, high intensity interval training, through changes in various mechanisms, lead to improvement in lipid and glycemic disorders, inflammation and appetite in obese people with type 2 diabetes. Hence, according to the findings of the study, researchers suggest that performing high intensity interval training can lead to significant improvements in obese subjects (with and without type 2 diabetes).

THE EFFECTS OF HIGH INTENSITY INTERVAL TRAINING ON SERUM LEVELS OF FGF21 AND INSULIN RESISTANCE IN OBESE MEN

Karim Azali Alamdari

Background: Fibroblast growth factor 21 (FGF21) plays an important role in regulation of energy metabolism and insulin resistance; however, the synergistic effects of high intensity interval training (HIIT) on FGF21 and insulin resistance are not elucidated which was focused in this study.
Methods: This experimental research was conducted by pretest- posttest design with control group. In this study, 24 obese men were assigned into two groups including
HIIT (n=12, age: 39.83 ± 5.63 years, weight: 100.83 ± 5.81 kg and body mass index: 32.73 ± 2.8 kg/m2) and Control (n=12, age: 39.41 ± 5.23 years, weight: 100.08 ± 4.71 kg, and body mass index: 32.34 ± 1.43 kg/m2). The HIIT group experienced eight weeks of intensive exercises (three sessions per week, four bouts in each session at the intensity of 85-90% of maximum heart rate (MHR) with 3-minute active rest intervals (at 60-50% of MHR) between exercise bouts. Blood samples were collected at baseline and also 48 hours following to the last training session. The data were compared using paired and independent samples t tests.
Results: HIIT resulted in a contemporary reduction of plasma glucose and serum insulin and FGF21 and also improved HOMA index (P˂0/05).
Conclusion: High intensity interval training plays an effective role in improvement of insulin resistance and reduction of serum FGF21 in obese subjects, which may be due to an improved FGF21 resistance.

PREVALENCE OF VITAMIN D DEFICIENCY AND ITS RELATED FACTORS IN RESIDENTS OF HAMADAN CITY

Ronak Hamzehei

Background: Vitamin D plays an important role in the health, survival and fertility of humans, This Vitamin has a significant role in calcium homeostasis and cellular growth and facilitating normal immune system function. In recent years, the role of vitamin D in various diseases has been highlighted. Regarding high prevalence of vitamin D deficiency in Iran, The aim of this study was to determine the prevalence and status of vitamin D of Hamadan.
Method: A cross-sectional descriptive study in a one-year period, 7816 patients were referred to the laboratories of Shahid Beheshti and Farshchian Sina Hospitals of Hamadan Were investigated. The data was analyzed using R-version 3.2.3 and at a significant level of 0.05. The modified effect of age and sex factors on vitamin D level was investigated using logistic regression model.
Results: The prevalence of vitamin D deficiency was 0.69 in Hamadan province. 20.2% had severe deficiency (less than 10 Ng/ml) vitamin D, 20.5% moderate deficiency (10 to 20 Ng /ml), 28.6% mild deficiency (20 to30 Ng/ml), the prevalence of this deficiency was 46.7% in men and 38.7% in women. According to the results, there is a significant relationship between sex and age with vitamin D levels (p-value <0.001).
Conclusion: Based on this study and other studies, vitamin D deficiency is common in Iran and requires further investigation to improve the quality of life and prevent complications from deficiency of this essential vitamin.