Iranian Journal of Diabetes and Lipid Disorders
مجله دیابت و متابولیسم ایران
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Medical Sciences
http://ijdld.tums.ac.ir
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اثر تمرین هوازی و کپسایسین بر بیان Pdx1 و GLUT2 بافت پانکراس موشهای تغذیه شده با رژیم غذایی پُرچرب
The Effect of Aerobic Exercise and Capsaicin on the Gene Expression of Pancreaticpdx1 and GLUT2 in Rats Fed High-Fat Diet
تخصصي
Special
پژوهشي
Research
<strong><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">مقدمه:</span></span></strong><span style="font-family:B Lotus;"><span style="font-size:12.0pt;"> شواهد نشان میدهد که رژیم غذایی پُرچرب (</span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">HFD</span></span></span><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">) باعث هیپرانسولینی و اختلال در عملکرد جزایر پانکراس همراه با مقاومت به انسولین در بافت چربی میشود. هدف از پژوهش حاضر ارزیابی اثر تمرین هوازی همراه با کپسایسین بر بیان </span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">Pdx1</span></span></span><span style="font-family:B Lotus;"><span style="font-size:12.0pt;"> و </span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">GLUT2</span></span></span><span style="font-family:B Lotus;"><span style="font-size:12.0pt;"> بافت پانکراس موشهای </span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">HFD</span></span></span><span style="font-family:B Lotus;"><span style="font-size:12.0pt;"> بود. </span></span><br>
<strong><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">روشها:</span></span></strong><span style="font-family:B Lotus;"><span style="font-size:12.0pt;"> در این مطالعهی تجربی، 40 سر موش صحرایی نر ویستار بهمدت 8 هفته با رژیم غذایی نرمال (8=</span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">n</span></span></span><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">، </span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">ND</span></span></span><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">) و </span></span><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">رژیم غذایی پُرچرب (</span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">HFD</span></span></span><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">) </span></span><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">(32=</span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">n</span></span></span><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">) تغذیه شدند. بعد از هشت هفته همه موشها به 5 گروه: رژیم غذایی نرمال (</span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">ND</span></span></span><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">)، </span></span><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">رژیم غذایی پُرچرب (</span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">HFD</span></span></span><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">)</span></span><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">، رژیم غذایی پُرچرب-تمرین (</span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">HFDT</span></span></span><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">)، رژیم غذایی پُرچرب-کپسایسین (</span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">HFDCap</span></span></span><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">) و رژیم غذایی پُرچرب-تمرین-کپسایسین (</span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">HFDTCap</span></span></span><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">) تقسیم شدند. گروههای تمرین بهمدت هشت هفته برنامهی دویدن هوازی با شدت متوسط (60-50% </span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">VO2max</span></span></span><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">، 25-15 متر/دقیقه، 60-30 دقیقه/روز، پنج روز/هفته) را روی تردمیل انجام دادند. کپسایسین (</span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">mg/kg/day</span></span></span><span style="font-family:B Lotus;"><span style="font-size:12.0pt;"> 4) یک بار در روز بهصورت خوراکی با گاواژ خورانده شد.</span></span> <span style="font-family:B Lotus;"><span style="font-size:12.0pt;">دادهها با استفاده از </span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">ANOVA</span></span></span><span style="font-family:B Lotus;"><span style="font-size:12.0pt;"> در سطح </span></span><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">معناداری </span></span><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">05/0 </span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">P<</span></span></span><span style="font-family:B Lotus;"><span style="font-size:12.0pt;"> آزمون شد.</span></span> <span style="font-family:B Lotus;"><span style="font-size:12.0pt;"></span></span><br>
<strong><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">یافتهها</span></span></strong><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">: نتایج نشان داد </span></span><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">بیان </span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">Pdx1</span></span></span><span style="font-family:B Lotus;"><span style="font-size:12.0pt;"> و </span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">GLUT2</span></span></span><span style="font-family:B Lotus;"><span style="font-size:12.0pt;"> د</span></span><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">ر گروههای </span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">HFDT</span></span></span><span style="font-family:B Lotus;"><span style="font-size:12.0pt;"> (بهترتیب 002/0=</span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">P</span></span></span><span style="font-family:B Lotus;"><span style="font-size:12.0pt;"> و 003/0=</span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">P</span></span></span><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">)، </span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">HFDCap</span></span></span><span style="font-family:B Lotus;"><span style="font-size:12.0pt;"> (بهترتیب 022/0=</span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">P</span></span></span><span style="font-family:B Lotus;"><span style="font-size:12.0pt;"> و 026/0=</span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">P</span></span></span><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">) و </span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">HFDTCap</span></span></span><span style="font-family:B Lotus;"><span style="font-size:12.0pt;"> (بهترتیب 000/0=</span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">P</span></span></span><span style="font-family:B Lotus;"><span style="font-size:12.0pt;"> و 000/0=</span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">P</span></span></span><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">) افزایش معنیداری داشت</span></span><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">. همچنین افزایش معنیداری در </span></span><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">بیان </span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">Pdx1</span></span></span><span style="font-family:B Lotus;"><span style="font-size:12.0pt;"> و </span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">GLUT2</span></span></span> <span style="font-family:B Lotus;"><span style="font-size:12.0pt;">در گروه </span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">HFDTCap</span></span></span><span style="font-family:B Lotus;"><span style="font-size:12.0pt;"> نسبت به </span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">HFDCap</span></span></span><span style="font-family:B Lotus;"><span style="font-size:12.0pt;"> (بهترتیب 037/0=</span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">P</span></span></span><span style="font-family:B Lotus;"><span style="font-size:12.0pt;"> و 044/0=</span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">P</span></span></span><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">) مشاهده شد. </span></span><br>
<strong><span dir="RTL"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">نتیجهگیری:</span></span></span></strong><span dir="RTL"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;"> نتایج نشان داد که </span></span></span><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">HFD</span></span><span dir="RTL"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;"> باعث اختلال در عملکرد سلولهای </span></span></span><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">β</span></span><span dir="RTL"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;"> پانکراس شده و </span></span></span><span dir="RTL"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">تمرین هوازی به تنهایی و همراه با کپسایسین </span></span></span><span dir="RTL"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">با افزایش </span></span></span><span dir="RTL"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">بیان </span></span></span><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">Pdx1</span></span><span dir="RTL"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;"> و </span></span></span><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">GLUT2</span></span><span dir="RTL"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">، احتمالا میتواند عملکرد سلولهای جزایر را بهبود ببخشد. </span></span></span>
<strong>Background:</strong> Evidence has suggested that high-fat diet (HFD) promote hyperinsulinemia and pancreatic islet dysfunction with insulin resistance in adipose tissue. The aim of the present study was to examine the effect of aerobic exercise and capsaicin on the gene expression of pancreaticPdx1 and GLUT2 in Rats HFD.<br>
<strong>Methods:</strong> this experimental study, 40 male Wistar rats were fed a normal diet (ND, n = 8) or high-fat diet (HFD) (n = 32) for 8 weeks. After 8 weeks, all rats were divided into 5 groups: normal diet (ND), high-fat diet (HFD), high-fat diet-training (HFDT), high-fat diet-capsaicin (HFDCap), high-fat diet-training-capsaicin (HFDTCap). Training groups have performed a moderate intensity aerobic running program (60-50% VO2max, at 15-25 m/min, 30-60 min/day, and 5 days/week) on a motor-driven treadmill for eight weeks. Capsaicin (4 mg/kg/day) were administered orally, by gavage, once a day. Data were analyzed using ANOVA at p<0.05.<br>
<strong>Results:</strong> the results showed significant increase in expression of Pdx1 and GLUT2 in HFDT (p = 0.002 and p = 0.003, respectively), HFDCap (p = 0.022 and p = 0.026, respectively) and HFDTCap (p = 0.000 and p = 0.000, respectively) groups.<span dir="RTL"></span><br>
<strong>Conclusion:</strong> also, a significant increase in the expression of Pdx1 and GLUT2 was observed in the HFDTCap compared to the HFDCap (respectively P=0.037, and p=0.044).<br>
The results showed that HFD impaired pancreatic beta cell function and that aerobic exercise alone and with capsaicin could significantly increase islet cell function by increasing Pdx1 and GLUT2 expression.
چاقی, فعالیت ورزشی, عملکرد پانکراس, گیاه دارویی
Obesity, Exercise, Pancreatic function, Herb
101
110
http://ijdld.tums.ac.ir/browse.php?a_code=A-10-378-3&slc_lang=fa&sid=1
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