Iranian Journal of Diabetes and Lipid Disorders
مجله دیابت و متابولیسم ایران
ijdld
Medical Sciences
http://ijdld.tums.ac.ir
1
admin
2345-4008
2345-4016
000
000
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000
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fa
jalali
1401
9
1
gregorian
2022
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22
5
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1
fulltext
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تأثیر تمرین استقامتی بر میزان پروتئینهای درگیر در تنظیم سوختوساز بافت چربی در رتهای دیابتی نوع دو
The Effect of Endurance Training On the Amount of Proteins Involved In the Regulation of Adipose Tissue Metabolism in Type 2 Diabetic Rats
عمومى
General
پژوهشي
Research
<span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Calibri",sans-serif"><b><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Lotus""><span style="color:black">مقدمه:</span></span></span></b><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Lotus""><span style="color:black"> پروتئینهای</span></span></span> <span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"><span style="color:black">mTOR</span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Lotus""><span style="color:black">و</span></span></span> <span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"><span style="color:black">CREB</span></span></span><span lang="FA" style="font-size:10.0pt"><span style="font-family:"B Lotus""><span style="color:black">دو</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Lotus""><span style="color:black"> عامل مهم در مسیرهای سلولی و تنظیم متابولیسم بافت چربی هستند. بنابراین هدف از مطالعهی حاضر، تأثیر تمرین استقامتی بر میزان پروتئینهای</span></span></span> <span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"><span style="color:black">mTOR</span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Lotus""><span style="color:black">و</span></span></span> <span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"><span style="color:black">CREB</span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Lotus""><span style="color:black">در بافت چربی رتهای دیابتی نوع دو است.</span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Calibri",sans-serif"><b><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Lotus""><span style="color:black">روشها:</span></span></span></b> <span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Lotus""><span style="color:black"><span style="letter-spacing:-.2pt">در این مطالعهی تجربی، 18 سر رت نر 2 ماهه از نژاد اسپراگ داولی با میانگین وزن 20</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"Cambria",serif"><span style="color:black"><span style="letter-spacing:-.2pt">±</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Lotus""><span style="color:black"><span style="letter-spacing:-.2pt">270 گرم انتخاب شدند. 12 سر از رتها از طریق تزریق درون صفاقی محلولهای </span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Lotus"">استرپتوزوتوسین <span style="color:black"><span style="letter-spacing:-.2pt">و نیکوتینآمید دیابتی نوع دو شدند. این رتها به روش تصادفی به 2 گروه، تمرین دیابتی (هر گروه 6 سر) و کنترل دیابتی تقسیم شدند؛ یک گروه کنترل سالم (6 سر) نیز درنظر گرفته شد؛ گروه تمرینی 4 روز در هفته مطابق با برنامهی تمرینی بهمدت 6 هفته به تمرین استقامتی پرداختند</span></span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="font-family:"Times New Roman",serif"><span style="color:black"><span style="letter-spacing:-.2pt">.</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Lotus""><span style="color:black"><span style="letter-spacing:-.2pt"> برای تجزیه و تحلیل دادهها از نرمافزار </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"><span style="color:black"><span style="letter-spacing:-.2pt">SPSS</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Lotus""><span style="color:black"><span style="letter-spacing:-.2pt"> نسخهی 23 و </span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Lotus""><span style="color:black"><span style="letter-spacing:-.2pt">آزمونهای آنوای-یکطرفه و تعقیبی توکی استفاده‏ شد.</span></span></span></span><b><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Lotus""><span style="color:black"><span style="letter-spacing:-.2pt"></span></span></span></span></b></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Calibri",sans-serif"><b><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Lotus""><span style="color:black">یافتهها:</span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Lotus""><span style="color:black"> محتوای پروتئین </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"><span style="color:black">mTOR</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Lotus""><span style="color:black"> بهدنبال 6 هفته تمرین <span style="letter-spacing:-.2pt">استقامتی</span>، تغییر معنیداری را نشان داد (0001/0</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"><span style="color:black">P</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="font-family:"Times New Roman",serif"><span style="color:black">=</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Lotus""><span style="color:black">)؛ آزمون تعقیبی توکی نشان داد این تغییر معنیدار بین جفت گروههای تمرین دیابتی نسبتبه کنترل سالم (004/0</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"><span style="color:black">P</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="font-family:"Times New Roman",serif"><span style="color:black">=</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Lotus""><span style="color:black">) و گروههای کنترل دیابتی نسبت به کنترل سالم است (0001/0</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"><span style="color:black">P</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="font-family:"Times New Roman",serif"><span style="color:black">=</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Lotus""><span style="color:black">). محتوای پروتئین </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"><span style="color:black">CREB</span></span></span><span style="font-size:12.0pt"><span style="font-family:"B Lotus""><span style="color:black"> تغییر معنیداری را نشان داد (0001/0</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"><span style="color:black">P</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="font-family:"Times New Roman",serif"><span style="color:black">=</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Lotus""><span style="color:black">)؛ این تغییر معنیدار بین جفت گروههای تمرین دیابتی نسبتبه کنترل دیابتی (02/0</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"><span style="color:black">P</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="font-family:"Times New Roman",serif"><span style="color:black">=</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Lotus""><span style="color:black">)، تمرین دیابتی نسبتبه کنترل سالم (0001/0</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"><span style="color:black">P</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="font-family:"Times New Roman",serif"><span style="color:black">=</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Lotus""><span style="color:black">) و گروههای کنترل دیابتی نسبتبه کنترل سالم (0001/0</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"><span style="color:black">P</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="font-family:"Times New Roman",serif"><span style="color:black">=</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Lotus""><span style="color:black">) بود.</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Lotus""><span style="color:black"></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Calibri",sans-serif"><b><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Lotus""><span style="color:black">نتیجهگیری:</span></span></span></b> <span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Lotus""><span style="color:black">پروتئینهای </span></span></span> <span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"><span style="color:black">mTOR</span></span></span><span lang="FA" style="font-size:10.0pt"><span style="font-family:"B Lotus""><span style="color:black">و </span></span></span> <span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"><span style="color:black">CREB</span></span></span><span lang="FA" style="font-size:10.0pt"><span style="font-family:"B Lotus""><span style="color:black">به</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Lotus""><span style="color:black">دنبال تمرین استقامتی کاهش یافتند که میتواند در تنظیم سوختوساز بافت چربی مؤثر باشند؛ با این وجود باید شرایط تمرینی بیشتری مدّ نظر قرار گیرد.</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Lotus""><span style="color:black"></span></span></span></span></span></span></span></span>
<span style="font-size:11pt"><span style="tab-stops:right 0cm"><span style="line-height:115%"><span style="font-family:"Calibri",sans-serif"><b><span style="font-family:"Times New Roman",serif">Background:</span></b><span style="font-family:"Times New Roman",serif"> mTOR and CREB proteins are two important factors in cellular pathways and regulating fat tissue metabolism. Therefore, the aim of this research is the effect of endurance training on the amount of mTOR and CREB proteins in the adipose tissue of type 2 diabetic rats.</span></span></span></span></span><br>
<span style="font-size:11pt"><span style="tab-stops:right 0cm"><span style="line-height:115%"><span style="font-family:"Calibri",sans-serif"><b><span style="font-family:"Times New Roman",serif">Methods:</span></b><span style="font-family:"Times New Roman",serif"> In this experimental study, 18 rats 2-month-old male Sprague-Dawley rats with a mean weight of 270±20g were selected. 12 rats became type 2 diabetic by intraperitoneal injection of Streptozotocin solutions. These rats were randomly divided into 2 groups: diabetic training and diabetic control (6 heads per group); A healthy control group (6 heads) was also considered. The training group practiced endurance training 4 days a week for 6 weeks. Data were analyzed using SPSS software version 23 and one-way ANOVA and Tukey post hoc tests.</span></span></span></span></span><br>
<span style="font-size:11pt"><span style="tab-stops:right 0cm"><span style="line-height:115%"><span style="font-family:"Calibri",sans-serif"><b><span style="font-family:"Times New Roman",serif">Results:</span></b><span style="font-family:"Times New Roman",serif"> mTOR protein content showed a significant change after 6 weeks of endurance training (P=0.0001); Tukey's post hoc test showed that this change was significant between the pairs of diabetic training groups to healthy controls (P=0.004) and diabetic control groups to healthy controls (P=0.0001). CREB protein content showed a significant change (P=0.0001); this change was significant between the pairs of diabetic training to diabetic control groups (P=0.02), diabetic training to healthy control (P=0.0001), and diabetic control to healthy control groups (P=0.0001).</span></span></span></span></span><br>
<span style="font-size:11pt"><span style="tab-stops:right 0cm"><span style="line-height:115%"><span style="font-family:"Calibri",sans-serif"><b><span style="font-family:"Times New Roman",serif">Conclusion:</span></b><span style="font-family:"Times New Roman",serif"> mTOR and CREB proteins decreased after Endurance Training, which can be effective in regulating adipose tissue metabolism; however, more training conditions should be considered.</span></span></span></span></span>
تمرین استقامتی, هدف مکانیکی راپامایسین در پستانداران, پروتئین اتصال عنصر پاسخگو به cAMP, بافت چربی, دیابت نوع دو
Endurance Training, Mammalian Target of Rapamycin, cAMP Response Element-Binding Protein, Fat Tissue, Type 2 Diabetes
321
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http://ijdld.tums.ac.ir/browse.php?a_code=A-10-1167-10&slc_lang=fa&sid=1
Sajad
Mirzaei
سجاد
میرزائی