Iranian Journal of Diabetes and Lipid Disorders
مجله دیابت و متابولیسم ایران
ijdld
Medical Sciences
http://ijdld.tums.ac.ir
1
admin
2345-4008
2345-4016
000
000
000
000
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fa
jalali
1401
12
1
gregorian
2023
3
1
22
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online
1
fulltext
fa
تأثیر تمرین با شدت بالا و مصرف بیتروت بر NRF1 و TFAM بافت چربی احشایی موشهای سالمند دیابتی نوع دو
The Effect of High Intensity Training and Beetroot Consumption on NRF1 and TFAM in Visceral Adipose Tissue of Aged Type 2 Diabetic Rats
تخصصي
Special
پژوهشي
Research
<span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Calibri",sans-serif"><b><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Lotus"">مقدمه</span></span></b><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Lotus"">: </span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif">NRF1</span></span><span style="font-size:12.0pt"><span style="font-family:"B Lotus""> و </span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif">TFAM</span></span><span style="font-size:12.0pt"><span style="font-family:"B Lotus""> از پروتئینهای مؤثر بر بایوژنز میتوکندریایی هستند که نقش مهمی در دیابت ایفا میکنند. هدف از مطالعه حاضر تبیین ت</span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Lotus"">أثیر تمرین</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Lotus"">با شدت بالا</span></span> <span dir="LTR" style="font-size:12.0pt"><span style="font-family:"Times New Roman",serif">(</span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif">HIT)</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Lotus"">و مصرف بیتروت بر </span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif">NRF1</span></span> <span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Lotus"">و </span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif">TFAM</span></span> <span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Lotus"">موشهای نر سالمند دیابتی نوع دو بود. </span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Calibri",sans-serif"><b><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Lotus""><span style="letter-spacing:-.1pt">روشها</span></span></span></b><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Lotus""><span style="letter-spacing:-.1pt">: در این مطالعهی تجربی، ۴۰ سر موش صحرایی نر (دامنهی وزن ۲۷۰-۳۰۰ گرم و سن ۱۲ تا ۱۴ هفته) دیابتی شده با استرپتوزوتوسین بهطور تصادفی به 5 گروه (کنترل سالم، کنترل دیابتی، مکمل، تمرین، تمرین+مکمل) تقسیم شدند. گروههای تمرین بهمدت ۴ هفته و هر هفته ۴ جلسه و هر جلسه ۴۴ دقیقه تحت تأثیر تمرین تناوبی شدید با شدت ۵۰ تا ۹۰ درصد حداکثر سرعت قرار گرفتند. گروههای دریافت کنندهی مکمل روزانه ۱۰ میلیگرم بیتروت به ازای هر کیلوگرم وزن بدن بهمدت ۴ هفته و ۴ بار در هفته بهصورت خوراکی دریافت کردند. دادهها با استفاده از آزمون تحلیل واریانس دو طرفه در سطح معنیداری (۰۵/۰</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"Times New Roman",serif"><span style="letter-spacing:-.1pt">≥</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"><span style="letter-spacing:-.1pt">P</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Lotus""><span style="letter-spacing:-.1pt">) ارزیابی شد.</span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Calibri",sans-serif"><b><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Lotus"">یافتهها</span></span></b><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Lotus"">: نتایج پژوهش نشان داد که القاء دیابت منجر به کاهش قابل توجه سطوح پروتئینی </span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif">NRF1</span></span><span style="font-size:12.0pt"><span style="font-family:"B Lotus""> و </span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif">TFAM</span></span><span style="font-size:12.0pt"><span style="font-family:"B Lotus""> شد بهطوری که در گروه تمرین و بیتروت اثر معنیداری بر تغییرات </span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif">NRF1</span></span><span style="font-size:12.0pt"><span style="font-family:"B Lotus""> در بافت چربی احشایی موشهای صحرایی سالمند مبتلا به دیابت دارند</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Lotus"">(۰۰۱/۰=</span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif">P</span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Lotus"">)</span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Lotus"">.</span></span> <span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Lotus"">اما تعامل تمرین </span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif">HIT</span></span><span style="font-size:12.0pt"><span style="font-family:"B Lotus""> و مکمل بیتروت در تغییرات </span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif">NRF1</span></span><span style="font-size:12.0pt"><span style="font-family:"B Lotus""> معنیدار نیست (۵۶/۰=</span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif">P</span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Lotus"">) و نیز عامل تمرین و مکمل بیتروت همچنین تعامل تمرین </span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif">HIT</span></span><span style="font-size:12.0pt"><span style="font-family:"B Lotus""> و بیتروت نیز اثر معنیداری بر تغییرات </span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif">TFAM</span></span><span style="font-size:12.0pt"><span style="font-family:"B Lotus""> در بافت چربی احشایی موشهای صحرایی سالمند مبتلا به دیابت دارند</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Lotus"">(۰۰۱/۰=</span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif">P</span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Lotus"">)</span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Lotus"">.</span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Calibri",sans-serif"><b><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Lotus"">نتیجهگیری</span></span></b><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Lotus"">: براساس یافتههای تحقیق حاضر بهنظر میرسد تمرین ورزشی تناوبی همراه با مصرف بیتروت میتواند به افزایش بیان ژن </span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif">NRF1</span></span><span style="font-size:12.0pt"><span style="font-family:"B Lotus""> و </span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif">TFAM</span></span><span style="font-size:12.0pt"><span style="font-family:"B Lotus""> منجر شود و در نتیجه به بهبود و کنترل دیابت در سالمندان کمک کند.</span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Calibri",sans-serif"><span dir="LTR" style="font-size:12.0pt"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span></span></span>
<span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:"Calibri",sans-serif"><b><span style="font-family:"Times New Roman",serif"><span style="color:black">Background</span></span></b><b><span style="font-family:"Times New Roman",serif">:</span></b><span style="font-family:"Times New Roman",serif"> NRF1 and TFAM are proteins that affect mitochondrial biogenesis and play an important role in diabetes. The aim of the present study was to investigate the effect of high intensity training (HIT) training and beetroot consumption on NRF1 and TFAM in visceral adipose tissue of aged type 2 diabetic rats.</span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:"Calibri",sans-serif"><b><span style="font-family:"Times New Roman",serif">Method:</span></b><span style="font-family:"Times New Roman",serif"> In this experimental study, 40 male rats (weight range 270-300 grams and age 12-14 weeks) diabetic with streptozotocin were randomly divided into 5 groups (healthy control, diabetic control, supplement, exercise, exercise + complement) were divided. The training groups were subjected to intense interval training with an intensity of 50 to 90% of the maximum speed for 4 weeks and 4 sessions each week and 44 minutes each session. The groups receiving the supplement received 10 mg of beetroot per kilogram of body weight daily for 4 weeks and 4 times a week orally. The data are statistically significant using two-way ANONA test and (P < 0.05) was evaluated. </span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:"Calibri",sans-serif"><b><span style="font-family:"Times New Roman",serif"><span style="color:black">Results</span></span></b><b><span style="font-family:"Times New Roman",serif">:</span></b><span style="font-family:"Times New Roman",serif"> The results of the study showed that the induction of diabetes led to a significant decrease in the protein levels of NRF1 and TFAM, so that in the exercise group, beetroot had a significant effect on the changes of NRF1 in the visceral fat tissue of elderly rats with diabetes. (P=0.001), but the interaction of HIT training and beetroot supplement in NRF1 changes is not significant (P=0.56) and also the factor of exercise and beetroot supplement, as well as the interaction of HIT training and beetroot also has a significant effect on TFAM changes in visceral adipose tissue of elderly rats with diabetes. (P=0.001).</span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:"Calibri",sans-serif"><b><span style="font-family:"Times New Roman",serif">Conclusion:</span></b><span style="font-family:"Times New Roman",serif"> Based on the findings of the present research, it seems that high interval training with beetroot consumption can lead to an increase in NRF1 and TFAM gene expression and thus help to improve and control diabetes in the elderly.</span></span></span></span>
بیتروت, تمرین HIT,NRF1 ,TFAM
Beetroot, HIT Training, NRF1, TFAM
361
371
http://ijdld.tums.ac.ir/browse.php?a_code=A-10-1882-1&slc_lang=fa&sid=1
Mahkameh
Abdollahi
مهکامه
عبدالهی