Iranian Journal of Diabetes and Lipid Disorders
مجله دیابت و متابولیسم ایران
ijdld
Medical Sciences
http://ijdld.tums.ac.ir
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2345-4008
2345-4016
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jalali
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2023
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بررسی تأثیر شش هفته تمرین هوازی بر سطوح پروتئینهای DCX و AMPA در هایپوکامپ موشهای صحرایی دیابتی
Studying the Effect of Six Weeks of Aerobic Exercise on the Protein Levels of DCX and AMPA in the Hippocampus of Diabetic Rats
تخصصي
Special
كاربردي
Applicable
<span style="font-size:11pt"><span style="line-height:95%"><span style="text-autospace:none"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Calibri",sans-serif"><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:95%"><span style="font-family:"B Lotus"">مقدمه:</span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:95%"><span style="font-family:"B Lotus""> از عوارض دیابت نوع دو اختلالات عصبشناختی است و پروتئینهای </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span style="font-family:"Times New Roman",serif">DCX</span></span></span><span style="font-size:12.0pt"><span style="line-height:95%"><span style="font-family:"B Lotus""> و </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span style="font-family:"Times New Roman",serif">AMPA</span></span></span><span style="font-size:12.0pt"><span style="line-height:95%"><span style="font-family:"B Lotus""> ممکن است در این اختلال درگیر باشند. بنابراین، هدف پژوهش بررسی اثر تمرین هوازی بر سطوح پروتئینهای </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span style="font-family:"Times New Roman",serif">DCX</span></span></span><span style="font-size:12.0pt"><span style="line-height:95%"><span style="font-family:"B Lotus""> و </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span style="font-family:"Times New Roman",serif">AMPA</span></span></span><span style="font-size:12.0pt"><span style="line-height:95%"><span style="font-family:"B Lotus""> در هایپوکمپ موشهای دارای دیابت نوع دو بود.</span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:95%"><span style="text-autospace:none"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Calibri",sans-serif"><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:95%"><span style="font-family:"B Lotus"">روشها:</span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:95%"><span style="font-family:"B Lotus""> در این پژوهش 32 سر موش نر ویستار هشت هفتهای به چهار گروه کنترل (</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span style="font-family:"Times New Roman",serif">C</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:95%"><span style="font-family:"B Lotus"">)، دیابت (</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span style="font-family:"Times New Roman",serif">D</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:95%"><span style="font-family:"B Lotus"">)، دیابت تمرین (</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span style="font-family:"Times New Roman",serif">DT</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:95%"><span style="font-family:"B Lotus"">) و تمرین (</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span style="font-family:"Times New Roman",serif">T</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:95%"><span style="font-family:"B Lotus"">) تقسیم شدند. دیابت به</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:95%"><span style="font-family:"Times New Roman",serif"></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:95%"><span style="font-family:"B Lotus"">وسیلهی تزریق استرپتوزوتوسین ایجاد شد. تمرین هوازی پنج جلسه در هفته برای شش هفته اجرا شد. 24 ساعت پس از آخرین جلسه موشها تشریح و بافت هایپوکمپ استخراج شد. پروتئین</span></span></span><span lang="AR-SA" style="font-family:"B Lotus""></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:95%"><span style="font-family:"B Lotus"">ها به روش الایزا اندازهگیری شد. برای تحلیل دادهها از آنالیز واریانس یکطرفه استفاده شد.</span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:95%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Calibri",sans-serif"><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:95%"><span style="font-family:"B Lotus"">یافته</span></span></span></b><b><span dir="LTR" style="font-size:12.0pt"><span style="line-height:95%"><span style="font-family:"Times New Roman",serif"></span></span></span></b><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:95%"><span style="font-family:"B Lotus"">ها:</span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:95%"><span style="font-family:"B Lotus""> بین سطوح </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span style="font-family:"Times New Roman",serif">DCX</span></span></span><span style="font-size:12.0pt"><span style="line-height:95%"><span style="font-family:"B Lotus""> گروه </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span style="font-family:"Times New Roman",serif">C</span></span></span><span style="font-size:12.0pt"><span style="line-height:95%"><span style="font-family:"B Lotus""> و گروه</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:95%"><span style="font-family:"Times New Roman",serif"></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:95%"><span style="font-family:"B Lotus"">های </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span style="font-family:"Times New Roman",serif">T</span></span></span><span style="font-size:12.0pt"><span style="line-height:95%"><span style="font-family:"B Lotus""> (19/0=</span></span></span><i><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span style="font-family:"Times New Roman",serif">p</span></span></span></i><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:95%"><span style="font-family:"B Lotus"">) و </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span style="font-family:"Times New Roman",serif">DT</span></span></span><span style="font-size:12.0pt"><span style="line-height:95%"><span style="font-family:"B Lotus""> (50/0=</span></span></span><i><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span style="font-family:"Times New Roman",serif">p</span></span></span></i><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:95%"><span style="font-family:"B Lotus"">) اختلاف معناداری وجود نداشت. اما بین سطوح </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span style="font-family:"Times New Roman",serif">DCX</span></span></span><span style="font-size:12.0pt"><span style="line-height:95%"><span style="font-family:"B Lotus""> گروه</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:95%"><span style="font-family:"Times New Roman",serif"></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:95%"><span style="font-family:"B Lotus"">های </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span style="font-family:"Times New Roman",serif">C</span></span></span><span style="font-size:12.0pt"><span style="line-height:95%"><span style="font-family:"B Lotus""> و </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span style="font-family:"Times New Roman",serif">D</span></span></span><span style="font-size:12.0pt"><span style="line-height:95%"><span style="font-family:"B Lotus""> تفاوت معناداری وجود داشت (05/0=</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span style="font-family:"Times New Roman",serif">P</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:95%"><span style="font-family:"B Lotus"">). بین گروههای دیابتی و گروه </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span style="font-family:"Times New Roman",serif">T</span></span></span><span style="font-size:12.0pt"><span style="line-height:95%"><span style="font-family:"B Lotus""> اختلاف معناداری مشاهده شد (05/0></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span style="font-family:"Times New Roman",serif">P</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:95%"><span style="font-family:"B Lotus"">). سطوح </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span style="font-family:"Times New Roman",serif">AMPA</span></span></span><span style="font-size:12.0pt"><span style="line-height:95%"><span style="font-family:"B Lotus""> گروه</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:95%"><span style="font-family:"Times New Roman",serif"></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:95%"><span style="font-family:"B Lotus"">های دیابتی به شکل معناداری کمتر از گروههای </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span style="font-family:"Times New Roman",serif">C</span></span></span><span style="font-size:12.0pt"><span style="line-height:95%"><span style="font-family:"B Lotus""> و </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span style="font-family:"Times New Roman",serif">T</span></span></span><span style="font-size:12.0pt"><span style="line-height:95%"><span style="font-family:"B Lotus""> بود (05/0></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span style="font-family:"Times New Roman",serif">P</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:95%"><span style="font-family:"B Lotus"">). تفاوت بین گروه</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:95%"><span style="font-family:"Times New Roman",serif"></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:95%"><span style="font-family:"B Lotus"">های </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span style="font-family:"Times New Roman",serif">C</span></span></span><span style="font-size:12.0pt"><span style="line-height:95%"><span style="font-family:"B Lotus""> و </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span style="font-family:"Times New Roman",serif">T</span></span></span><span style="font-size:12.0pt"><span style="line-height:95%"><span style="font-family:"B Lotus""> (21/0=</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span style="font-family:"Times New Roman",serif">P</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:95%"><span style="font-family:"B Lotus"">) و گروه</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:95%"><span style="font-family:"Times New Roman",serif"></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:95%"><span style="font-family:"B Lotus"">های </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span style="font-family:"Times New Roman",serif">D</span></span></span><span style="font-size:12.0pt"><span style="line-height:95%"><span style="font-family:"B Lotus""> و </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span style="font-family:"Times New Roman",serif">DT</span></span></span><span style="font-size:12.0pt"><span style="line-height:95%"><span style="font-family:"B Lotus""> (73/0=</span></span></span><i><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span style="font-family:"Times New Roman",serif">p</span></span></span></i><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:95%"><span style="font-family:"B Lotus"">) معنادار نبود. بین </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span style="font-family:"Times New Roman",serif">AMPA</span></span></span><span style="font-size:12.0pt"><span style="line-height:95%"><span style="font-family:"B Lotus""> و </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span style="font-family:"Times New Roman",serif">DCX</span></span></span><span style="font-size:12.0pt"><span style="line-height:95%"><span style="font-family:"B Lotus""> با گلوکز همبستگی منفی معناداری مشاهده شد (بهترتیب 0005/0=</span></span></span><i><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span style="font-family:"Times New Roman",serif"> p</span></span></span></i><span style="font-size:12.0pt"><span style="line-height:95%"><span style="font-family:"B Lotus""> ، 75/0-=</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:95%"><span style="font-family:"Times New Roman",serif">r</span></span></span><span style="font-size:12.0pt"><span style="line-height:95%"><span style="font-family:"B Lotus""> و 0005/0=</span></span></span><i><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span style="font-family:"Times New Roman",serif"> p</span></span></span></i><span style="font-size:12.0pt"><span style="line-height:95%"><span style="font-family:"B Lotus""> ، 65/0-=</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span style="font-family:"Times New Roman",serif">r</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:95%"><span style="font-family:"B Lotus"">).</span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:95%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Calibri",sans-serif"><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:95%"><span style="font-family:"B Lotus"">نتیجهگیری:</span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:95%"><span style="font-family:"B Lotus""> دیابت پروتئین</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:95%"><span style="font-family:"Times New Roman",serif"></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:95%"><span style="font-family:"B Lotus"">های </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span style="font-family:"Times New Roman",serif">AMPA</span></span></span><span style="font-size:12.0pt"><span style="line-height:95%"><span style="font-family:"B Lotus""> و </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:95%"><span style="font-family:"Times New Roman",serif">DCX</span></span></span><span style="font-size:12.0pt"><span style="line-height:95%"><span style="font-family:"B Lotus""> را کاهش می</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:95%"><span style="font-family:"Times New Roman",serif"></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:95%"><span style="font-family:"B Lotus"">دهد اما ورزش اثر دیابت بر آنها را بهطور غیرمعناداری کاهش میدهد. با توجه بهمدت زمان مناسب تمرین، این احتمال وجود دارد با توجه به ارتباط منفی معنادار این دو پروتئین با گلوکز خون، شدت تمرین بتواند اثر منفی دیابت روی آنها را کاهش دهد.</span></span></span></span></span></span></span></span>
<span style="font-size:11pt"><span style="line-height:115%"><span style="font-family:"Calibri",sans-serif"><b><span style="font-family:"Times New Roman",serif">Background:</span></b><span style="font-family:"Times New Roman",serif"> One of the complications of type 2 diabetes is the development of neurocognitive disorders, and DCX and AMPA may be involved in this disorder. Therefore, this study aimed to investigate the effect of aerobic exercise on the DCX and AMPA in the hippocampus of rats with type 2 diabetes.</span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:115%"><span style="font-family:"Calibri",sans-serif"><b><span style="font-family:"Times New Roman",serif">Methods:</span></b><span style="font-family:"Times New Roman",serif"> 32 eight-week-old male rats were divided into control (C), diabetes (D), diabetes-exercise (DT), and exercise (T) groups. Diabetes was induced by streptozotocin injection. The exercise was carried out for six weeks. Finally, the rats were dissected, and their hippocampus tissue was extracted. Proteins were measured by the ELISA method.</span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:115%"><span style="font-family:"Calibri",sans-serif"><b><span style="font-family:"Times New Roman",serif">Results:</span></b><span style="font-family:"Times New Roman",serif"> There was nonsignificant difference between the DCX of the C group and the T and DT groups (</span><i><span style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"Times New Roman",serif">p</span></span></span></i><span style="font-family:"Times New Roman",serif">>0.05). But there was a significant difference between the DCX of the C and diabetic groups (</span><i><span style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"Times New Roman",serif">p</span></span></span></i><span style="font-family:"Times New Roman",serif"> =0.05). Also, a significant difference was observed between the diabetic groups and the T group (</span><i><span style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"Times New Roman",serif">p</span></span></span></i><span style="font-family:"Times New Roman",serif"><0.05). AMPA in diabetic groups were significantly lower than in C and T groups (</span><i><span style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"Times New Roman",serif">p</span></span></span></i><span style="font-family:"Times New Roman",serif"><0.05) While the difference between C and T groups and D and DT groups was not significant (</span><i><span style="font-size:10.0pt"><span style="line-height:115%"><span style="font-family:"Times New Roman",serif">p</span></span></span></i><span style="font-family:"Times New Roman",serif">>0.05). Also, a significant negative correlation was observed between AMPA and DCX with blood glucose.</span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:115%"><span style="font-family:"Calibri",sans-serif"><b><span style="font-family:"Times New Roman",serif">Conclusion:</span></b><span style="font-family:"Times New Roman",serif"> Diabetes reduces the AMPA and DCX but exercise nonsignificantly reduces the effect of diabetes on those. According to the appropriate duration of exercise, there is a possibility that due to the significant negative correlation between these proteins and glucose, the intensity of exercise can significantly reduce the negative effect of diabetes on those.</span></span></span></span>
دیابت, تمرین هوازی, هایپوکمپ, AMPA, DCX
Diabetes, Aerobic Exercise, Hippocampus, AMPA, DCX
372
383
http://ijdld.tums.ac.ir/browse.php?a_code=A-10-1048-4&slc_lang=fa&sid=1
Saeed
Naimi
سعید
نعیمی