Iranian Journal of Diabetes and Lipid Disorders
مجله دیابت و متابولیسم ایران
ijdld
Medical Sciences
http://ijdld.tums.ac.ir
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2345-4008
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jalali
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2023
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تأثیر شش هفته تمرین تناوبی شدید همراه با مصرف مکمل کوآنزیم Q10 بر مقادیر Nrf2 و NQO1در عضله قلب رتهای نر سالمند دیابتی
The Effect of Six Weeks High Intensity Interval Training along with Taking Coenzyme Q10 Supplement on Nrf2 and, NQO1 in Heart Muscle of Old Male Diabetic Rats
تخصصي
Special
پژوهشي
Research
<span style="font-size:11pt"><span style="line-height:92%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="color:black">مقدمه:</span></span></span></span></b><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="color:black"> بیماری دیابت منجر به عوارض جانبی متعدد در سیستم قلبی-عروقی و نیز عملکرد نامناسب سیستم آنتی</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span cambria="" style="font-family:"><span style="color:black"></span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="color:black">اکسیدانی بدن می</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span cambria="" style="font-family:"><span style="color:black"></span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="color:black">شود. هدف از پژوهش حاضر بررسی تأثیر شش هفته تمرین تناوبی با شدت بالا همراه با مصرف مکمل کوآنزیم </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><span style="color:black">Q10</span></span></span></span><span style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="color:black"> بر مقادیر </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><span style="color:black">Nrf2</span></span></span></span> <span lang="FA" style="font-size:10.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="color:black">و</span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><span style="color:black">NQO1</span></span></span></span><span style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="color:black"> عضلۀ قلب رت</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span cambria="" style="font-family:"><span style="color:black"></span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="color:black">های سالمند دیابتی است.</span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:92%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="color:black">روشها:</span></span></span></span></b> <span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">در این مطالعۀ تجربی </span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">12 سر رت نر سالمند (18ماهه) بهطور تصادفی در چهار گروه تمرین هوازی شدید همراه با مصرف مکمل کوآنزیم </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">Q10</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">، گروه تمرین هوازی شدید، گروه مصرف مکمل کوآنزیم </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">Q10</span></span></span><span style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"> و گروه کنترل قرار گرفتند. </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">القاء دیابت با تزریق تک دوز استرپتوزوتوسین به مقدار60 میلیگرم برکیلوگرم به روش درون صفاقی انجام شد. برنامۀ تمرین هوازی بهمدت شش هفته انجام شد.</span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">گروه مکمل کوآنزیم </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">Q10</span></span></span><span style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"> با دوز 200 </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">mg/kg</span></span></span><span style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"> بهصورت خوراکی دریافت کردند.</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">برای اندازهگیری </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">مقادیر</span></span></span> <span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">Nrf2</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">، </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">NQO1</span></span></span><span style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"> از روش وسترن بلات استفاده شد. دادهها به روش آزمون تحلیل واریانس یکطرفه و آزمون تعقیبی توکی در</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">سطح معنیداری </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">05/0</span></span></span> <span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">P</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><</span></span></span><span style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"> تجزیه</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">وتحلیل شد</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">.</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="color:black"><span style="letter-spacing:-.2pt"></span></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:92%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="color:black">یافتهها:</span></span></span></span></b> <span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">نتایج نشان داد مکمل کوآنزیم </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">Q10</span></span></span><span style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"> (<span style="color:black">014/0</span></span></span></span> <span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><span style="color:black">P</span></span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><span style="color:black">=</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="color:black">)</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">، تمرین تناوبی شدید (<span style="color:black">001/0 </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><span style="color:black">P</span></span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><span style="color:black">=</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="color:black">)</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"> و تمرین تناوبی شدید به همراه مصرف مکمل کوآنزیم </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">Q10</span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">(<span style="color:black">001/0</span></span></span></span> <span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><span style="color:black">P</span></span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><span style="color:black">=</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="color:black">)</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"> بهطور معنیداری باعث افزایش مقادیر</span></span></span> <span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">Nrf2</span></span></span><span style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"> در قلب رتهای سالمند دیابتی میشود. همچنین مصرف مکمل کوآنزیم </span></span></span> <span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">Q10</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">(<span style="color:black">0366/0</span></span></span></span> <span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><span style="color:black">P</span></span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><span style="color:black">=</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="color:black">)</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"> ، تمرین تناوبی شدید (<span style="color:black">014/0</span></span></span></span> <span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><span style="color:black">P</span></span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><span style="color:black">=</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="color:black">) </span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">و تمرین تناوبی شدید به همراه مصرف مکمل کوآنزیم </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">Q10</span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">(<span style="color:black">002</span></span></span></span><span lang="FA" style="font-size:14.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="color:black">/0</span></span></span></span> <span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><span style="color:black">P</span></span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><span style="color:black">=</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="color:black">)</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"> بهطور معنیداری باعث افزایش مقادیر </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">NQO1</span></span></span><span style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"> در قلب رتهای سالمند دیابتی شد.</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="color:black"></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:92%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="color:black">نتیجهگیری:</span></span></span></span></b> <span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">تمرین تناوبی شدید بههمراه مصرف مکمل، از طریق افزایش آنزیمهای آنتیاکسیدانی درونزا موجب بهبود عملکرد قلب در بیماران سالمند دیابتی میشود.</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="color:black"></span></span></span></span></span></span></span></span></span>
<span style="font-size:11pt"><span style="line-height:normal"><span style="tab-stops:right 0cm"><span sans-serif="" style="font-family:Calibri,"><b><span new="" roman="" style="font-family:" times="">Background:</span></b> <span lang="EN" new="" roman="" style="font-family:" times="">Diabetes leads to numerous side effects in the cardiovascular system and also to improper functioning of the body's antioxidant system. The aim of this study is to investigate the effect of six weeks of high-intensity interval training with coenzyme Q10 supplementation on the values of Nrf2 and NQO1 in heart muscle of elderly diabetic rats.</span><span style="font-family:"Times New Roman","serif""></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="tab-stops:right 0cm"><span sans-serif="" style="font-family:Calibri,"><b><span new="" roman="" style="font-family:" times="">Methods:</span></b><span new="" roman="" style="font-family:" times=""> In this experimental study, 48 elderly male rats (18 months old) were randomly divided into four groups of intense aerobic exercise with coenzyme Q10 supplementation, intense aerobic exercise group, coenzyme Q10 supplement intake group, and control group. Induction of diabetes was done by injecting a single dose of streptozotocin in the amount of 60 mg/kg intraperitoneally. The program of </span><span new="" roman="" style="font-family:" times="">high intensity interval training</span><span lang="EN" new="" roman="" style="font-family:" times=""> was done for six weeks. Coenzyme Q10 supplement group received orally at a dose of 200 mg/kg. Western blot method was used to measure Nrf2 and NQO1 values. The data were analyzed by one-way analysis of variance and Tukey's post hoc test at a significance level of P <0.05. </span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="tab-stops:right 0cm"><span sans-serif="" style="font-family:Calibri,"><b><span new="" roman="" style="font-family:" times="">Results:</span></b> <span lang="EN" new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.3pt">The results showed taking coenzyme Q10 supplement (P= 0.014), intense intermittent exercise (P= 0.001)</span></span><span lang="EN" new="" roman="" style="font-family:" times=""> and intense intermittent exercise with coenzyme Q10 supplement (P= 0.001) significantly increased Nrf2 values in the hearts of diabetic elderly rats. Also, the use of coenzyme Q10 supplement (P=0.0366), intense intermittent exercise (P= 0.014) and intense intermittent exercise along with coenzyme Q10 supplement use (P= 0.002)</span> <span lang="EN" new="" roman="" style="font-family:" times="">significantly increased the amount of NQO1 in the hearts of diabetic aged male rats.</span><span lang="AR-SA" dir="RTL" style="font-family:"Times New Roman","serif""></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="tab-stops:right 0cm"><span sans-serif="" style="font-family:Calibri,"><b><span new="" roman="" style="font-family:" times="">Conclusion:</span></b> <span lang="EN" new="" roman="" style="font-family:" times="">Intermittent intense training along with supplement consumption improves heart function in elderly diabetic patients through increasing endogenous antioxidant enzymes.</span><span lang="AR-SA" dir="RTL" style="font-family:"Times New Roman","serif""></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="tab-stops:right 0cm"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-family:"Times New Roman","serif""></span></b></span></span></span></span>
دیابت, سالمندی, تمرین تناوبی شدید, مکمل کوآنزیم Q10, Nrf2, NQO1
Diabetes, Aging- High Intensity Interval Training, Q10, Nrf2, NQO1
114
124
http://ijdld.tums.ac.ir/browse.php?a_code=A-10-1854-1&slc_lang=fa&sid=1
Moazameh
Malekpour
معظمه
ملک پور