Iranian Journal of Diabetes and Lipid Disorders
مجله دیابت و متابولیسم ایران
ijdld
Medical Sciences
http://ijdld.tums.ac.ir
1
admin
2345-4008
2345-4016
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000
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fa
jalali
1402
8
1
gregorian
2023
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23
4
online
1
fulltext
fa
تأثیر تمرین تناوبی با شدت بالا بر میزان پروتئینهای اتوفاژی خانواده بکلین 1/2 (BECLIN1/2) در بطن چپ قلب رتهای مبتلا به دیابت نوع یک
The Effect of High-Intensity Interval Training on the Amount of BECLIN1/2 Family Autophagy Proteins in the Left Ventricle of the Heart in Rats with Type 1 Diabetes
تخصصي
Special
پژوهشي
Research
<span style="font-size:11pt"><span style="line-height:92%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="color:black">مقدمه</span></span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="color:black">: </span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="color:black">چندین پروتئین مسیر اتوفاژی را تنظیم میکنند </span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="color:black">و یکی از مهمترین تنظیمکنندهها پروتئینهای خانوادۀ </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><span style="color:black">BECLIN</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="color:black"> است. بنابراین، این تحقیق با هدف بررسی تأثیر تمرین تناوبی پرشدت (</span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><span style="color:black">HIIT</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="color:black">) بر میزان پروتئینهای اتوفاژی خانوادۀ </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><span style="color:black">BECLIN۱/۲</span></span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="color:black">در بطن چپ قلب موشهای صحرایی مبتلا به دیابت نوع یک انجام شد.</span></span></span></span><b><span dir="LTR" style="font-size:12.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><span style="color:black"></span></span></span></span></b></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="line-height:92%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span style="color:black"><b><span lang="AR-SA" style="line-height:92%"><span b="" lotus="" style="font-family:">روشها: </span></span></b><span lang="FA" style="line-height:92%"><span b="" lotus="" style="font-family:">در این مطالعۀ تجربی، ۱۸ سر رت نر سه ماهه از نژاد اسپراگداولی با میانگین وزن ۲۰</span></span><span lang="FA" style="line-height:92%"><span cambria="" style="font-family:">±</span></span><span lang="FA" style="line-height:92%"><span b="" lotus="" style="font-family:">۳۰۰ گرم انتخاب شدند. ۱۲ سر از رت از طریق تزریق درونصفاقی محلول استرپتوزوتوسین مبتلا به دیابت نوع یک شدند. این رتها به روش تصادفی به دو گروه، تمرین دیابتی و کنترل دیابتی تقسیم شدند؛ یک گروه کنترل سالم نیز درنظر گرفته شد؛ </span></span><span dir="LTR" lang="X-NONE" style="font-size:10.0pt"><span style="line-height:92%">HIIT</span></span><span lang="FA" style="line-height:92%"><span b="" lotus="" style="font-family:"> بهمدت ۶ هفته و هر هفته ۴ جلسه شامل ۵ وهله ۴ دقیقهای با شدت معادل ۸۵ تا ۹۵ درصد حداکثر سرعت و دورههای استراحت فعال ۳ دقیقهای با شدت معادل ۵۰ تا ۶۰ درصد حداکثر سرعت انجام شد. تجزیهوتحلیل دادهها از طریق آزمونهای آنوای-یکطرفه و تعقیبی توکی در نرمافزار </span></span><span dir="LTR" lang="X-NONE" style="font-size:10.0pt"><span style="line-height:92%">SPSS</span></span><span lang="FA" style="line-height:92%"><span b="" lotus="" style="font-family:"> نسخۀ ۲۶ انجام شد. سطح معناداری ۰۵/۰ در نظر گرفته شد.</span></span></span></span></span></span></span></span><br>
<span style="font-size:12pt"><span style="line-height:92%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span style="color:black"><b><span lang="FA" style="line-height:92%"><span b="" lotus="" style="font-family:">یافتهها: </span></span></b><span lang="FA" style="line-height:92%"><span b="" lotus="" style="font-family:">محتوای درون سلولی پروتئین </span></span><span dir="LTR" lang="X-NONE" style="font-size:10.0pt"><span style="line-height:92%">BECLIN۱</span></span><span lang="FA" style="line-height:92%"><span b="" lotus="" style="font-family:"> و </span></span><span dir="LTR" lang="X-NONE" style="font-size:10.0pt"><span style="line-height:92%">BECLIN۲</span></span><span lang="FA" style="line-height:92%"><span b="" lotus="" style="font-family:"> پس از شش هفته </span></span><span dir="LTR" lang="X-NONE" style="font-size:10.0pt"><span style="line-height:92%">HIIT</span></span><span lang="FA" style="line-height:92%"><span b="" lotus="" style="font-family:">، بین گروههای پژوهش در بطن چپ قلب کاهش معناداری را نشان داد (۰۰۰۱/۰ =</span></span><span dir="LTR" lang="X-NONE" style="font-size:10.0pt"><span style="line-height:92%">P</span></span><span lang="FA" style="line-height:92%"><span b="" lotus="" style="font-family:">).</span></span><span lang="AR-SA" style="line-height:92%"><span style="font-family:"B Lotus""></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:92%"><span style="tab-stops:right 0cm"><span style="direction:rtl"><span style="unicode-bidi:embed"><span sans-serif="" style="font-family:Calibri,"><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">نتیجهگیری: </span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">بهنظر میرسد پروتئینهای خانوادۀ </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">BECLIN</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"> از طریق </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">HIIT</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"> کاهش مییابند و این میتواند سازِکار اتوفاژی را در سلولهای قلبی کاهش دهد.</span></span></span><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""></span></span></span></b></span></span></span></span></span></span>
<span style="font-size:11pt"><span style="line-height:normal"><span style="text-autospace:none"><span sans-serif="" style="font-family:Calibri,"><b><span new="" roman="" style="font-family:" times="">Background:</span></b><b> </b><span new="" roman="" style="font-family:" times="">Several proteins regulate the autophagy pathway, and one of the most important regulators is the BECLIN family proteins. Therefore, this research aims to investigate the effect of high-intensity interval training on the amount of BECLIN1/2 family autophagy proteins in the left ventricle of the heart of rats with type 1 diabetes. </span><b><span style="font-family:"TimesNewRomanPSMT","sans-serif""></span></b></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="text-autospace:none"><span sans-serif="" style="font-family:Calibri,"><b><span new="" roman="" style="font-family:" times="">Methods: </span></b><span new="" roman="" style="font-family:" times="">In this experimental study, 18 three-month-old male Sprague Dawley rats with an average weight of 300±20 grams were selected. 12 rats were diagnosed with type 1 diabetes through intraperitoneal injection of streptozotocin solution. These rats were randomly divided into two groups, diabetic exercise, and diabetic control; A healthy control group was also considered; The HIIT was performed for 6 weeks and 4 sessions each week including 5 bursts of 4 minutes with an intensity equal to 85-95% of the maximum speed and 3-minute active rest periods with an intensity equal to 50-60% of the maximum speed. Data analysis was done through one-way ANOVA and Tukey's post hoc tests in SPSS version 26 software. A significance level of 0.05 was considered.</span><span new="" roman="" style="font-family:" times=""><span style="color:black"></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><span new="" roman="" style="font-family:" times=""><span style="color:black">Results</span></span></b><b><i><span new="" roman="" style="font-family:" times=""><span style="color:black">:</span></span></i></b><span new="" roman="" style="font-family:" times=""><span style="color:black"> The intracellular content of BECLIN1 and BECLIN2 protein showed a significant decrease between the research groups in the left ventricle of the heart after six weeks of HIIT (<i>p</i>=0.0001).</span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="text-autospace:none"><span sans-serif="" style="font-family:Calibri,"><b><span lang="EN" new="" roman="" style="font-family:" times="">Conclusion:</span></b><span lang="EN" new="" roman="" style="font-family:" times=""> It seems that BECLIN family proteins are decreased by HIIT and this can decrease the autophagy mechanism in cardiac cells.</span></span></span></span></span>
پروتئینهای بکلین 1/2, تمرین تناوبی پرشدت, دیابت نوع یک, بطن چپ قلب
BECLIN 1/2 Proteins, High-Intensity Interval Training, Type 1 Diabetes, Left Ventricle Heart
236
244
http://ijdld.tums.ac.ir/browse.php?a_code=A-10-1699-1&slc_lang=fa&sid=1
Hamed
Alizadeh Palavani
حامد
علیزاده پهلوانی
ha.alizadeh@cfu.ac.ir