Iranian Journal of Diabetes and Lipid Disorders
مجله دیابت و متابولیسم ایران
ijdld
Medical Sciences
http://ijdld.tums.ac.ir
1
admin
2345-4008
2345-4016
000
000
000
000
000
fa
jalali
1402
9
1
gregorian
2023
12
1
23
5
online
1
fulltext
fa
اثر تعاملی هشت هفته تمرین هوازی و مکملیاری ویتامین D3 بر سطوح پروتئین آیریزین قلبی، مقاومت به انسولین و پروفایل لیپیدی در رتهای القاء شده به دیابت نوع دو
The Interactive Effect of Eight Weeks of Aerobic Training and Vitamin D3 Supplementation on Cardiac Irisin Protein Levels, Insulin Resistance and Lipid Profile in Rats Induced with Type 2 Diabetes
تخصصي
Special
پژوهشي
Research
<span style="font-size:11pt"><span style="line-height:92%"><span style="tab-stops:right 0cm"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,"sans-serif""><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="letter-spacing:-.2pt">مقدمه:</span></span></span></span></b><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="letter-spacing:-.2pt"> پژوهش حاضر با هدف بررسی اثر هشت هفته تمرین هوازی و مکمل</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"Cambria","serif""><span style="letter-spacing:-.2pt"></span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="letter-spacing:-.2pt">یاری ویتامین </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif""><span style="letter-spacing:-.2pt">D3</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="letter-spacing:-.2pt"> بر سطح پروتئین آیریزین قلبی، مقاومت به انسولین و پروفایل لیپیدی در رت</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"Cambria","serif""><span style="letter-spacing:-.2pt"></span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="letter-spacing:-.2pt">های القاء شده به دیابت نوع دو انجام شد.</span></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:92%"><span style="tab-stops:right 0cm"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,"sans-serif""><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="letter-spacing:-.2pt">روشها:</span></span></span></span></b> <span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">چهل سر رت نر نژاد ویستار پس از القاء دیابت نوع دو از طریق ترکیب رژیم غذایی پُرچرب+ استرپتوزوتوسین، بهطور تصادفی به پنج گروه: 1) کنترل سالم (</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">NC</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">)، 2) کنترل دیابتی (</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">DC</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">)، 3) دیابت+تمرین هوازی (</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">DAT</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">)، 4) دیابت+ویتامین </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">D3</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""> (</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">DVD</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">) و 5)دیابت+ تمرین هوازی+ ویتامین </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">D3</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""> (</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">DVDAT</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">) تقسیم شدند. برنامۀ تمرین هوازی (پنجبار در هفته با شدت 60% حداکثر سرعت دویدن، بهمدت یک ساعت) و مکمل یاری ویتامین </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">D3</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""> (هر هفته 10000</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">IU</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">/ کیلوگرم) انجام شد. پس از بیهوش نمودن رتها، نمونۀ خونی جهت ارزیابی سطوح سرمی گلوکز، انسولین، تریگلیسیرید، کلسترول، </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">LDL</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""> و </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">HDL</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""> گرفته شد. بافت قلب جهت ارزیابی مقدار محتوای پروتئینی آیریزین جداسازی گردید.</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:92%"><span style="tab-stops:right 0cm"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,"sans-serif""><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="letter-spacing:-.2pt">یافتهها:</span></span></span></span></b> <span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">نتایج تحلیل واریانس یکطرفه نشان داد که سطح پروتئین آیریزین بافت قلب در گروههای </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">DAT</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">، </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">DVD</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""> و </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">DVDAT</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""> افزایش معنادار 04/14، 28/12 و 56/24 درصدی بهترتیب نسبت به </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">DC</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""> داشت (001/0></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">P</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">)،</span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">مقاومت به انسولین نیز در تمامی گروههای مداخله نسبت به گروه </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">DC</span></span></span><span style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""> کاهش معناداری نشان داد (001/0></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">P</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">)، اما سطوح </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">HDL</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">، تری</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif""></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">گلیسیرید و کلسترول فقط در گروههای </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">DAT</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""> و </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">DVDAT</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""> نسبت به گروه </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">DC</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""> کاهش معناداری داشت.</span></span></span><i><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="letter-spacing:-.2pt"></span></span></span></span></i></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:92%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,"sans-serif""><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""><span style="letter-spacing:-.2pt">نتیجهگیری:</span></span></span></span></b><b> </b><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">با توجه به نتایج، بهنظر میرسد که هشت هفته تمرین هوازی و مکمل ویتامین </span></span></span> <span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span style="font-family:"Times New Roman","serif"">D3</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus"">احتمالاً</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:92%"><span style="font-family:"B Lotus""> از طریق افزایش محتوای پروتئین آیریزین قلبی، باعت بهبود شاخص مقاومت به انسولین و پروفایل لیپیدی در رتهای القاء شده به دیابت نوع دو میگردد. </span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="tab-stops:right 0cm"><span style="line-height:107%"><span style="font-family:Calibri,"sans-serif""><b><span style="font-family:"Times New Roman","serif"">Background:</span></b><span style="font-family:"Times New Roman","serif""> <span style="background:white">The present study was conducted to investigate the effect of eight weeks of aerobic training and vitamin D3 supplementation on cardiac irisin protein levels, insulin resistance and lipid profile in rats induced with type 2 diabetes.</span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:Calibri,"sans-serif""><b><span style="font-family:"Times New Roman","serif""><span style="letter-spacing:.65pt">Methods:</span></span></b> <span style="font-family:"Times New Roman","serif"">Forty male Wistar rats after induction of type 2 diabetes through the combination of high-fat diet +streptozotocin were randomly divided into five groups: 1) Normal control (NC) ,2) Diabetic Control (DC), 3) Diabetes+ Aerobic Training (DAT) ,4) Diabetes+ Vitamin D3 (DVD) and 5) Diabetes+ Aerobic Training+ Vitamin D3 (DVDAT). Aerobic training program (five times a week with 60% intensity of maximum running speed for one hour) and vitamin D3 supplementation (10000 IU/kg every week) were performed. After anesthetizing the rats, blood samples were taken to evaluate serum glucose, insulin, triglyceride, cholesterol, LDL and HDL levels. The heart tissue was isolated to evaluate the irisin.protein.</span><sup><span style="font-size:12.0pt"><span style="letter-spacing:-.2pt"></span></span></sup></span></span></span><br>
<span style="font-size:11pt"><span style="tab-stops:right 0cm"><span style="line-height:107%"><span style="font-family:Calibri,"sans-serif""><b><span style="font-family:"Times New Roman","serif"">Results:</span></b><span style="font-family:"Times New Roman","serif""> <span style="letter-spacing:-.2pt">The results of the one way analysis of variance showed that the irisin protein level of the heart tissue in DAT, DVD and DVDAT groups had a significant increase of 14.04, 12.28 and 24.56%, respectively compared to DC (P<0.001). Insulin resistance also showed a significant decrease in all intervention groups compared to the DC group (P<0.001); However, HDL, triglyceride and cholesterol levels were significantly decreased in DAT and DVDAT groups compared to DC group (P<0.001).</span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="tab-stops:right 0cm"><span style="line-height:107%"><span style="font-family:Calibri,"sans-serif""><b><span style="font-family:"Times New Roman","serif"">Conclusion:</span></b><span style="font-family:"Times New Roman","serif""> According to the results, it seems that eight weeks of aerobic training and vitamin D3 supplementation probably improves the insulin resistance index and lipid profile in rats induced to type 2 diabetes through increasing the cardiac irisin.</span></span></span></span></span>
دیابت, تمرین هوازی, ویتامین D3, تمرین استقامتی, آیریزین
Diabetes, Aerobic training, Vitamin D3, Endurance training, Irisin
327
337
http://ijdld.tums.ac.ir/browse.php?a_code=A-10-1996-1&slc_lang=fa&sid=1
Hadi
Golpasandi
هادی
گلپسندی