Iranian Journal of Diabetes and Lipid Disorders
مجله دیابت و متابولیسم ایران
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Medical Sciences
http://ijdld.tums.ac.ir
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2024
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تأثیر ۱۲ هفته تمرین مقاومتی همراه با دریافت مکمل سماق بر سطوح سرمی سیرتویینهای میتوکندریایی ۳ و ۴ و آدنوزین مونوفسفات کیناز موشهای نر آلزایمری
The Effect of 12 Weeks of Resistance Training with Sumac Supplementation on the Serum Levels of Mitochondrial Sirtuins 3 and 4 and Adenosine Monophosphate Kinase in Alzheimer's Male Rats
تخصصي
Special
پژوهشي
Research
<span style="font-size:11pt"><span style="line-height:92%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span style="background:white"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="color:black">مقدمه:</span></span></span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span style="background:white"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="color:black"> بیماری آلزایمر شایعترین نوع زوال عقل است که بهصورت پیشرونده با ازدستدادن حافظه و واکنش به محیط همراه است. هدف این مطالعه بررسی تأثیر ۱۲ هفته تمرین مقاومتی همراه با دریافت سماق بر سطح سرمی </span></span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">SIR3</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">، </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">SIR4</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"> و </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">AMPK</span></span></span><span style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"> <span lang="AR-SA" style="background:white"><span style="color:black">در موشهای نر آلزایمری بود.</span></span><span lang="AR-SA" style="background:white"></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:92%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span style="background:white"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="color:black">روش</span></span></span></span></span></b><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">ها<span style="background:white"><span style="color:black">:</span></span></span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"> در این مطالعۀ تجربی، موشهای صحرایی بـا سـن ۱۰-۸ هفته و میـانگین وزنـی ۳۰</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span cambria="" style="font-family:">±</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">۲۳۰</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">گرم بهصورت تصادفی به پنج گروه کنترل، آلزایمری، آلزایمری همراه با دریافت مکمل سماق، آلزایمری همراه با تمرین مقاومتی و آلزایمری همراه با تمرین مقاومتی و دریافت مکمل سماق تقسیم شدند. برای القای آلزایمر، ۸ میلیگرم/کیلوگرم تری متیل تین کلراید به همراه ۲۰۰ میکرولیتر نرمال سالین بهصورت درون صفاقی به موشها تزریق شد. تمرین مقاومتی بهمدت ۱۲ هفته و ۵ روز در هفته روی نردبان ویژۀ موش انجام گرفت. </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="background:white"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="color:black">۴۸ ساعت بعد از آخرین جلسۀ تمرین، </span></span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">نمونۀ خونی گرفته شد. سطح سرمی </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">SIR3</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">، </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">SIR4</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"> و </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">AMPK</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"> به روش الایزا مورد ارزیابی قرار گرفتند. برای بررسی تغییرات بین گروه کنترل با سایر گروهها از آزمون </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">T</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"> مستقل و برای بررسی تفاوت سایر گروهها از آزمون آنالیز واریانس یکسویه و در صورت معنیداری از آزمون تعقیبی توکی، </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">توسط نرمافزار </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">SPSS</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"> نسخۀ ۲۹ </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">در سطح معنیداری ۰۵/۰</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">P</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"> استفاده شد.</span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:92%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">یافتهها:</span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"> نتایج تحقیق حاضر نشان داد که ۱۲ هفته تمرین مقاومتی موجب افزایش میزان </span></span></span> <span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">SIR3</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">(036/0 </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">P</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">)، </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">SIR4</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"> (029/0 </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">P</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">) و </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">AMPK</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"> (447/0 </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">P</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">) نسبت به گروه آلزایمری شده شد. بهعلاوه، اضافهکردن سماق به برنامۀ تمرین مقاومتی باعث تغییرات در میزان </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">SIR3</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"> (048/0 </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">P</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">)، </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">SIR4</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"> (024/0 </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">P</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">) و </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">AMPK</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"> (002/0 </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times="">P</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:92%"><span new="" roman="" style="font-family:" times=""><</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:">) شد.</span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:92%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span style="background:white"><span style="line-height:92%"><span b="" lotus="" style="font-family:"><span style="color:black">نتیجهگیری:</span></span></span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:92%"><span b="" lotus="" style="font-family:"> نتایج ما پیشنهاد میکند که تمرین مقاومتی با بهبود سطح سیرتوئین ۳، سیرتوئین ۴ و آدنوزین مونوفسفات کیناز در موشهای آلزایمری همراه است و دریافت ترکیب آنتیاکسیدانی سماق منجر به مضاعف شدن این پاسخها میشود.</span></span></span></span></span></span></span></span>
<span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:107%"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Background: </span></b><span new="" roman="" style="font-family:" times="">Alzheimer's disease is the most common type of dementia, which is associated with progressive loss of memory and reaction to the environment. The aim of this study was to investigate the effect of 12 weeks of resistance training with sumac intake on the serum levels of SIR3, SIR4 and AMPK in Alzheimer's male rats.</span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:107%"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Methods: </span></b><span new="" roman="" style="font-family:" times="">In this experimental study, male Wistar rats with an age of 8-10 weeks and an average weight of 230±30 grams were randomly divided into five groups: control, Alzheimer's disease, Alzheimer's disease with sumac supplementation, Alzheimer's disease with resistance training, and Alzheimer's disease with resistance training. Received sumac supplements were divided. To induce Alzheimer's disease, 8 mg/kg of trimethyl tin chloride along with 200 microliters of normal saline were injected intraperitoneally to mice. Resistance training was performed for 12 weeks and 5 days a week on a special rat ladder. 48 hours after the last training session, a blood sample was taken. Serum levels of SIR3, SIR4 and AMPK were evaluated by ELISA method. To check the changes between the control group and other groups using the independent T-test and to check the difference between the other groups using the one-way analysis of variance test and if significant, using the Tukey post hoc test, by SPSS version 29 software at a significance level of P< 0.05 used.</span><span lang="AR-SA" dir="RTL" style="font-family:"B Zar""></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:107%"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Results: </span></b><span new="" roman="" style="font-family:" times="">The results of this research showed that 12 weeks of resistance training increases the levels of SIR3 (P< 0.036), SIR4 (P< 0.029) and AMPK (P< 0.447) compared to the Alzheimer group. In addition, adding sumac to the resistance training program had a significant effect on the levels of SIR3 (P< 0.078), SIR4 (P< 0.024) and AMPK (P< 0.002).</span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:107%"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Conclusion: </span></b><span new="" roman="" style="font-family:" times="">Our results suggest that resistance training is associated with the improvement of sirtuin 3, sirtuin 4 and adenosine monophosphate kinase levels in Alzheimer's rats and receiving sumac antioxidant compound leads to doubling of these responses.</span></span></span></span></span></span>
آلزایمر, تمرین مقاومتی, سماق, سیرتوئین, آدنوزین مونوفسفات کیناز
Alzheimer's, Resistance Training, Sumac, Sirtuin, Adenosine Monophosphate Kinase
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http://ijdld.tums.ac.ir/browse.php?a_code=A-10-114-4&slc_lang=fa&sid=1
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