Iranian Journal of Health and Environment
سلامت و محیط زیست
ijhe
Medical Sciences
http://ijhe.tums.ac.ir
1
admin
2008-2029
2008-3718
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fa
jalali
1401
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gregorian
2023
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fulltext
fa
اثرات آنتی ژنوتوکسیتی آویشن شیرازی بر آسیب DNA ناشی از H2O2 در لنفوسیتهای انسانی با استفاده از روش کامت
Antigenotoxicity effects of Zataria multiflora Boiss on H2O2-induced DNA damage in human lymphocytes by comet assay
عمومى
General
پژوهشي
Research
<div style="text-align: justify;"><span style="font-size:11pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:107%"><span style="font-family:Calibri,sans-serif"><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" lotus="" style="font-family:">زمینه و هدف</span></span></span></b><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" lotus="" style="font-family:">: </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" lotus="" style="font-family:">مطالعه حاضر با هدف بررسی اثرات محافظتی عصارههای آبی و اتانولی آویشن شیرازی (</span></span></span><i><span dir="LTR" style="line-height:107%"><span new="" roman="" style="font-family:" times="">Zataria multiflora Boiss</span></span></i><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" lotus="" style="font-family:">) بر آسیب</span></span></span> <em><span dir="LTR" style="line-height:107%">DNA</span></em> <span style="font-size:12.0pt"><span style="line-height:107%"><span b="" lotus="" style="font-family:"> لنفوسیت</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" lotus="" style="font-family:">های</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" lotus="" style="font-family:">انسان با استفاده از روش کامت، انجام شد.</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span style="font-family:"B Lotus""></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:115%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">روش بررسی:</span></span></span></b><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:"> لنفوسیتهای خون محیطی از 16 </span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">داوطلب سالم جدا شد. سلولها ابتدا با</span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">100 میکرومولار </span></span></span><span dir="LTR" style="line-height:115%"><span new="" roman="" style="font-family:" times="">H<sub>2</sub>O<sub>2</sub></span></span><sub> </sub><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">و نیز عصارههای آبی و الکلی برگ آویشن با غلظت </span></span></span><span dir="LTR" style="line-height:115%"><span new="" roman="" style="font-family:" times="">mg/mL</span></span><span style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:"> 2/5 بطور جداگانه تیمار شدند. نهایتاً سلولها در ترکیبی از </span></span></span><span dir="LTR" style="line-height:115%"><span new="" roman="" style="font-family:" times="">H<sub>2</sub>O<sub>2</sub></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:"> جهت ایجاد آسیب </span></span></span><span dir="LTR" style="line-height:115%"><span new="" roman="" style="font-family:" times="">DNA</span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:"> با عصاره آبی و الکلی در دمای </span></span></span><span dir="LTR" style="line-height:115%"><span new="" roman="" style="font-family:" times="">C</span></span><sup><span dir="LTR" style="line-height:115%"><span new="" roman="" style="font-family:" times="">0</span></span></sup><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:"> 4 به مدت </span></span></span><span dir="LTR" style="line-height:115%"><span new="" roman="" style="font-family:" times="">min</span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:"> 30، انکوبه شدند. میزان مهاجرت</span></span></span><span dir="LTR" style="line-height:115%"><span new="" roman="" style="font-family:" times=""> DNA </span></span><span style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:"> در سلولها با استفاده از روش کامت اندازه گیری گردید و آسیب</span></span></span><span dir="LTR" style="line-height:115%"><span new="" roman="" style="font-family:" times=""> DNA </span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">به صورت سه شاخص: طول دنباله، درصد</span></span></span><span dir="LTR" style="line-height:115%"><span new="" roman="" style="font-family:" times=""> DNA </span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" lotus="" style="font-family:">در دنباله و اندازه حرکت دنباله بیان شد.</span></span></span><em> </em> <span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"B Lotus""></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:107%"><span style="font-family:Calibri,sans-serif"><b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" lotus="" style="font-family:">یا</span></span></span></b><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" lotus="" style="font-family:">فته</span></span></span></b><b><span dir="LTR" style="line-height:107%"><span new="" roman="" style="font-family:" times=""></span></span></b><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" lotus="" style="font-family:">ها</span></span></span></b><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" lotus="" style="font-family:">: </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" lotus="" style="font-family:">نتایج نشان داد که آسیب</span></span></span> <span dir="LTR" style="line-height:107%">DNA</span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" lotus="" style="font-family:">لنفوسیتهای در معرض عصاره اتانولی</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" lotus="" style="font-family:">آویشن شیرازی به طور قابل توجهی کمتر از لنفوسیتهای تیمار شده با </span></span></span><span dir="LTR" style="line-height:107%"><span new="" roman="" style="font-family:" times="">H<sub>2</sub>O<sub>2</sub></span></span><sub> </sub><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" lotus="" style="font-family:">بود</span></span></span><span dir="LTR" style="line-height:107%"><span new="" roman="" style="font-family:" times="">.</span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" lotus="" style="font-family:"> علاوه بر این، طول دم (</span></span></span><span dir="LTR" style="line-height:107%"><span new="" roman="" style="font-family:" times="">µm</span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" lotus="" style="font-family:">) 1/62</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span cambria="" style="font-family:">±</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" lotus="" style="font-family:">5/48 در مقابل 6/90</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span cambria="" style="font-family:">±</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" lotus="" style="font-family:">22/82، </span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" lotus="" style="font-family:">درصد </span></span></span><span dir="LTR" style="line-height:107%">DNA</span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" lotus="" style="font-family:"> در دم 1/55</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span cambria="" style="font-family:">±</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" lotus="" style="font-family:">4/56 در مقابل 4/55</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span cambria="" style="font-family:">±</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" lotus="" style="font-family:">16/00، و اندازه حرکت دم (</span></span></span><span dir="LTR" style="line-height:107%"><span new="" roman="" style="font-family:" times="">µm</span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" lotus="" style="font-family:">) 0/11</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span cambria="" style="font-family:">±</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" lotus="" style="font-family:">0/28</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" lotus="" style="font-family:">در مقابل 0/83</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span cambria="" style="font-family:">±</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" lotus="" style="font-family:">2/33</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" lotus="" style="font-family:">بود (0/001 </span></span></span><span dir="LTR" style="line-height:107%"><span new="" roman="" style="font-family:" times="">p <</span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" lotus="" style="font-family:">).</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span style="font-family:"B Lotus""></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:107%"><span style="font-family:Calibri,sans-serif"><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" lotus="" style="font-family:">نتیجه­گیری:</span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" lotus="" style="font-family:"> نتایج نشان داد که عصارههای آبی و اتانولی</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" lotus="" style="font-family:">آویشن شیرازی</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" lotus="" style="font-family:">به طور قابل توجهی قادر به حذف رادیکالهای</span></span></span><span dir="LTR" style="line-height:107%"><span new="" roman="" style="font-family:" times=""> DPPH </span></span><span style="font-size:12.0pt"><span style="line-height:107%"><span b="" lotus="" style="font-family:"> از طریق مکانیسم احتمالی فعالیت مهار رادیکال هستند</span></span></span><span dir="LTR" style="line-height:107%"><span new="" roman="" style="font-family:" times="">.</span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" lotus="" style="font-family:"> در نتیجه، عصاره اتانولی اثر بهتری نسبت به عصاره آبی</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" lotus="" style="font-family:">آویشن شیرازی</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" lotus="" style="font-family:">در جلوگیری از آسیب اکسیداتیو</span></span></span> <span dir="LTR" style="line-height:107%">DNA</span><em><span dir="LTR" style="line-height:107%"> </span></em><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" lotus="" style="font-family:">به لنفوسیتهای انسانی داشت</span></span></span><span dir="LTR" style="line-height:107%"><span new="" roman="" style="font-family:" times="">.</span></span><span dir="LTR" style="line-height:107%"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span></span></span></div>
<div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:150%"><span style="text-autospace:none"><span style="font-family:Calibri,sans-serif"><b><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">Background and Objective: </span></span></b><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">The present study aimed to explore the shielding effects of aqueous and ethanolic extracts of <i>Zataria multiflora</i> <i>Boiss</i> on human lymphocyte DNA damage using the comet assay</span></span><span dir="RTL" lang="FA" style="font-size:12.0pt"><span style="line-height:150%"><span b="" lotus="" style="font-family:">.</span></span></span><span style="line-height:150%"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:150%"><span style="text-autospace:none"><span style="font-family:Calibri,sans-serif"><b><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">Materials and Methods:</span></span></b> <span lang="EN" style="line-height:150%"><span new="" roman="" style="font-family:" times="">This study was conducted as a laboratory experiment to investigate the protective effects of thyme in preventing DNA damage. </span></span><span lang="EN" style="line-height:150%"><span new="" roman="" style="font-family:" times="">Peripheral blood lymphocytes were isolated from 16 healthy volunteers. First, the cells were treated with 100 μM H<sub>2</sub>O<sub>2</sub> and aqueous and alcoholic extracts of thyme leaves with a concentration of 2.5 mg/mL separately. Finally, the cells were incubated in a mixture of H<sub>2</sub>O<sub>2</sub> to cause DNA damage with each of the two aqueous and alcoholic extracts at 4°C for 30 minutes. The amount of DNA migration in cells was measured using the Comet method, and DNA damage was expressed as three indicators: sequence length, percentage of DNA in the sequence, and the amount of sequence movement.</span></span><span dir="RTL" lang="AR-SA" style="font-size:12.0pt"><span style="line-height:150%"><span style="font-family:"B Lotus""></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:150%"><span style="text-autospace:none"><span style="font-family:Calibri,sans-serif"><b><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">Results:</span></span></b><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""> The results showed that the DNA damage</span></span><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""> of</span></span><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""> lymphocytes exposed to the </span></span><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">ethanolic extract of<i> Z. multiflora Boiss</i> was significantly lesser than the </span></span><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">H<sub>2</sub>O<sub>2-</sub>treated </span></span><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">lymphocytes</span></span><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">. In addition, tail length (µm) was 5.48±1.62 versus 22.82±6.90, tail DNA (%) was 4.56±1.55 in contrast to 16.00±4.55, and tail moment (µm) was 0.28±0.11 against 2.33±0.83 (p < 0.001).</span></span><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""> T</span></span><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">he results showed that <i>Z.multiflora Boiss</i> aqueous and ethanolic extracts were significantly able to scavenge DPPH radicals through a possible radical scavenging activity mechanism.</span></span><span dir="RTL" lang="FA" style="font-size:12.0pt"><span style="line-height:150%"><span style="font-family:"B Lotus""></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:150%"><span style="text-autospace:none"><span style="font-family:Calibri,sans-serif"> <b><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">Conclusion:</span></span></b><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""> As a result, the ethanolic extract had a better effect than the aqueous extract of <i>Z. multiflora Boiss</i> in preventing oxidative DNA damage to human lymphocytes.</span></span></span></span></span></span></div>
آسیب DNA, لنفوسیتهای انسانی, عصاره آویشن شیرازی, روش کامت
DNA damage, Human lymphocytes, Zataria multiflora Boiss, Comet assay
737
750
http://ijhe.tums.ac.ir/browse.php?a_code=A-10-253-3&slc_lang=fa&sid=1
Nabi
Shariatifar
نبی
شریعتی فر
nshariati@tums.ac.ir