Dermatology and Cosmetic
پوست و زیبایی
jdc
Medical Sciences
http://jdc.tums.ac.ir
1
admin
2008-7470
2228-7388
000
000
000
000
000
fa
jalali
1401
2
1
gregorian
2022
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1
13
1
online
1
fulltext
fa
تأثیر عصاره آلوئهورا و نانوذرات سنتزشده سبز در کاهش التهاب در پسوریازیس: یک مطالعه in vitro
The effect of aloe vera extract and synthesized green nanoparticles on reducing inflammation in psoriasis: an in vitro study
تخصصي
Special
پژوهشي
Research
<span style="font-size:10pt"><span style="page-break-after:auto"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman""><span class="AbstractBoldChar" style="font-family:"Times New Roman Bold""><span style="font-weight:bold"><span lang="AR-SA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">زمینه و هدف:</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Mitra""> پسوریازیس یک بیماری التهابی پوستی مزمن، تحتتأثیر فاکتورهای ژنتیکی و مبتنیبر ایمنی است که با التهاب پوست همراه است. ژل آلوئهورا بهطور سنتی برای درمان بیماریهای پوستی ازجمله پسوریازیس استفاده میشود.</span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Mitra""></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="page-break-after:auto"><span style="line-height:6pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman""><span lang="AR-SA" style="font-size:13.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="page-break-after:auto"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman""><span class="AbstractBoldChar" style="font-family:"Times New Roman Bold""><span style="font-weight:bold"><span lang="AR-SA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">روش اجرا: </span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Mitra"">در این مطالعه عصاره اتانولی ژل آلوئهورا برای فعالیت ضدالتهابی و آنتیاکسیدانی در سلولهای </span></span>A431<span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Mitra""> مشتقشده از پوست مورد ارزیابی قرار گرفت. در ابتدا پس از تهیه عصاره و در مرحله بعد سنتز سبز نانوذرات طلا با استفاده از عصاره، تأثیر آنها را در بیان ژنهای التهابی و همچنین خاصیت آنتیاکسیدانی بررسی شد.</span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="page-break-after:auto"><span style="line-height:6pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman""><span lang="AR-SA" style="font-size:13.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="page-break-after:auto"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman""><span class="AbstractBoldChar" style="font-family:"Times New Roman Bold""><span style="font-weight:bold"><span lang="AR-SA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">یافتهها:</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Mitra""> استفاده از غلظتهای مختلف عصاره آلوئهورا سبب افزایش نسبی در سطوح بیان </span></span>EGFR<span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Mitra""> و </span></span>Nrf2<span style="font-size:12.0pt"><span style="font-family:"B Mitra""> و کاهش بیان ژنهای التهابی </span></span>NF-κB<span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Mitra"">،</span></span> TNF-α <span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Mitra"">و</span></span> IL-6 <span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Mitra"">در سلولهای </span></span>A431<span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Mitra""> پوست شد؛ اما تأثیر نانوذرات سنتزشده در مقایسه با عصاره آلوئهورا در تغییر بیان ژنهای سیگنالینگ و التهابی بیشتر بود.</span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="page-break-after:auto"><span style="line-height:6pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman""><span lang="AR-SA" style="font-size:13.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span></span><br>
<span class="AbstractBoldChar" style="font-family:"Times New Roman Bold""><span style="font-weight:bold"><span dir="RTL" lang="AR-SA" style="font-size:11.0pt"><span style="font-family:"B Mitra"">نتیجهگیری:</span></span></span></span><span dir="RTL" lang="AR-SA" style="font-size:13.0pt"><span style="font-family:"B Nazanin""> عصاره و نانوذرات سنتزشده خاصیت آنتیاکسیدانی داشتند و همچنین سبب کاهش بیان ژنهای التهابی در سلولها شدند؛ البته تأثیر نانوذرات نسبت به عصاره در کاهش ژنهای التهابی خیلی بیشتر بود.</span></span>
<p><span style="font-size:11pt"><span style="line-height:110%"><span style="font-family:"Times New Roman""><span class="NormalLeftBoldChar" style="font-family:"Times New Roman Bold""><span style="font-weight:bold">Background and Aim: </span></span>Psoriasis is a chronic inflammatory skin disease, influenced by genetic and immune-based factors that is associated with skin inflammation. Aloe vera gel is traditionally used to treat skin diseases including psoriasis.</span></span></span><br>
</p>
<p><span style="font-size:11pt"><span style="line-height:110%"><span style="font-family:"Times New Roman""><span class="NormalLeftBoldChar" style="font-family:"Times New Roman Bold""><span style="font-weight:bold">Methods:</span></span> In this study, ethanolic extract of aloe vera gel was evaluated for its anti-inflammatory and antioxidant activity in skin-derived A431 cells. First, after preparing the extract and then the green synthesis of gold nanoparticles using the extract, their effect on the expression of inflammatory genes as well as their antioxidant properties were investigated. </span></span></span><br>
</p>
<p><span style="font-size:11pt"><span style="line-height:110%"><span style="font-family:"Times New Roman""><span class="NormalLeftBoldChar" style="font-family:"Times New Roman Bold""><span style="font-weight:bold">Results:</span></span> The use of different concentrations of aloe vera extract caused a relative increase in EGFR and Nrf2 expression levels and decreased expression of NF-κB, TNF-α and IL-6 inflammatory genes in A431 skin cells. But the effect of synthesized nanoparticles was greater than aloe vera extract in altering the expression of signaling and inflammatory genes.</span></span></span><br>
</p>
<p><span class="NormalLeftBoldChar" style="font-family:"Times New Roman Bold""><span style="font-weight:bold"><span style="font-size:11.0pt">Conclusion:</span></span></span><span style="font-size:11.0pt"><span style="font-family:"Times New Roman""> The synthesized extracts and nanoparticles had antioxidant properties and also reduced the expression of inflammatory genes in cells. However, the effect of nanoparticles was much greater than that of extracts in reducing inflammatory genes.</span></span></p>
پسوریازیس, آلوئهورا, نانوذرات, بیان ژن
psoriasis, aloe vera, nanoparticles, gene expression
29
39
http://jdc.tums.ac.ir/browse.php?a_code=A-10-1-292&slc_lang=fa&sid=1
Houshang
Nemati
هوشنگ
نعمتی
hnemati@kums.ac.ir