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Showing 3 results for Candida Albicans
Evaluation of antimicrobial effect of four disinfectant solutions on handpieces contaminated to staphylococcus aureus , pseudomonas aeruginosa and candida albicans
M. Vahedi, P. Bakianian Vaziri , Hr. Abdolsamadi, A. Pahlavan, M. Hajilooii, Sh. Abdollahzadeh,
Volume 21, Issue 2 (11-2008)
Abstract

Background and Aim: Contaminated dental handpieces have the potential to transfer infection to patients. New generation of autoclavable turbines have to some extent overcome the problem however, in clinic sometimes it is necessary to use chemical to disinfect handpieces. The aim of this study was to determine the antimicrobial effect of some routinely used disinfectant solutions on dental contaminated handpieces.

Materials and Methods: In this experimental study 35 turbines were sterilized. The turbines' heads were inserted into microbial suspension containing staphylococcus, pseudomonas and candida and then exposed to the disinfectant solutions. Inoculations into culture medium were made at different intervals. All medium was incubated for 24 hours at 37oC followed by spectrophotometer inspection for detection of microbial growth. Serial dilutions of disinfectant agents were used to determine the highest dilution in which there was no microbial growth. Kruskal wallis test was used for statistical analysis and p<0.05 was considered as the level significance.

Results: Ethanol had antimicrobial effect on all of the tested microorganisms at dilution of 1:4. Betadine at dilution of 1:64 caused inhibition of all of the microbes except pseudomonas. Micro 10 had antimicrobial effect up to dilution of 1:256 but could not inhibit microbial growth at higher dilution. Sodium hypochlorite inhibited growth of the three microoganisms up to dilution of 1:1024.

Conclusion: Based of on the results of this study sodium hypochlorite was found to be the most effective antimicrobial agent among those used in this study, inhibiting microbial growth at the highest dilution.


Effect of sodium bicarbonate against Candida albicans in denture stomatitis: An in vitro study
Shamsolmoulouk Najafi, Reza Yazdani, Behzad Salari, Homa Forouhesh Tehrani, Mohammad Javad Kharrazi Fard,
Volume 29, Issue 2 (11-2016)
Abstract

Background and Aims: The aim of this study was to investigate the effects of sodium bicarbonate against candida albicans, the main infectious factor of denture stomatitis, and to compare it with other common anti-candida medications.

Materials and Methods: Three concentrations of Candida albicans (105, 106, 107 CFU/mL) were prepared. Experimental substances were 5% sodium bicarbonate, nystatin, chlorhexidine, and sterile saline (as control). Each of them was added separately to the same amounts of Candida albicans of different concentrations, and sampled at the intervals of 1, 2, 3 and 4 min. Then, the samples were cultured and incubated. The number of formed colonies was counted for each plate. The data were analyzed, using ANOVA and Tukey test.

Results: Sodium bicarbonate showed a noticeable anti-candida effect on the concentrations of 105 and 106 CFU/mL, with almost similar anti-candida effect as nystatin and chlorehexidine(P=0.29, P=0.32). Nystatin was the most effective medication on the concentration of 107 CFU/mL of candida (P=0.03, P=0.01). Sodium bicarbonate and chlorhexidine were less effective in this concentration with no statistically significant difference (P=1.00).

Conclusion: Sodium bicarbonate showed a significant anti-candida effect at low and medium concentrations of Candida albicans, but nystatin was more effective in the higher concentrations.


Molecular study of Candida albicans isolated from periodontal infections and effect of Sargasum alga extract on biofilm als gene expression using Real-Time-PCR
Pooneh Mahmoudi, Kiumarss Amini, Parviz Amini,
Volume 32, Issue 1 (7-2019)
Abstract
Background and Aims: Candida is an opportunistic pathogen that causes illness in people with a defective or weakened condition. Infectious diseases (periodontal diseases) are inflammatory and malignant inflammation of the dental-gum complex, in which the growth of biofilms caused by Candida glabrata, Parapseilosis and Tropikalis is less than Candida albicans. Brown algae Sargasum is more compatible with human medicines due to having a natural origin than chemical drugs and has less side effects. The aim of the present study was to investigate the molecular characteristics of Candida species isolated from periodontal infections and the effect of Sargassum glaucescens extract on biofilm gene expression using Real-Time-PCR.
Materials and Methods: Oral samples of periodontal infection were collected from the referred patients. To isolate the candidate species, the specimens were cultured on a Sabouraud dextrose agar containing chloramphenicol (SDAc). The extracted DNA was extracted from colonies grown from Kit and Glass pearl. Grown chickens were identified by specific primers by PCR-RFLP method. In order to detect the expression of als genes in Candida isolates, RNA extraction was performed using Phenol-Chloroform and Pearl glass, and the CLSI-M27-A2 method was used to evaluate the effect of Sargasum glaucescens extract of algae.
Results: The results showed that the expression of als gene in periodontal infection is higher than other genes. Another role is als in the formation of Candida albicans biofilm. The minimum inhibitory concentration of fungal growth was 256 μg/ml by algae extract. Sargasum glaucescens reduced the expression of als gene expression by about 62% in the sample.
Conclusion: Sargasum glaucescens algae possesses specific pharmacological properties and antimicrobial and antimicrobial effects. The results of the study using Real Time PCR showed that expression of als gene in isolated studied with Sargasum algae extract was lower than untreated isolates. Thus, this indicated the positive role of treatment by sargasum glaucescens extract in reducing the expression of biofilm gene in isolates.

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