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Showing 7 results for Enterococcus Faecalis

M. Zarrabian , M. Aligholi , N. Loghmani Nejad ,
Volume 18, Issue 2 (5-2005)

Statement of Problem: Root canal cleaning and elimination of the source of infection are the most important basis and the main requirements for successful root treatment since the main cause of failure in root treatment is the permeation of bacteria or their products into the periapical tissues. Nowadays, the newly designed and prcsented instruments for canal instrumentation can improve root treatment.

Purpose: The aim of this study was to compare the decrease in the number of intracanal Enterococcus-faecalis (Ef) among three mechanical instrumentation methods: manual (K-type) and rotary (Race, Profile).

Materials and Methods: In this experimental study, 30 single rooted teeth were selected. Three cases were considered as negative and three cases as posetive controls and 24 remainder cases were divided into three experimental groups. All root canals were inoculated by Ef and samples were taken from all canals to prepare microbial cultures. The three instrumentation procedures were: - Crown- down technique with K-type manual system file - Crown- down technique with Profile rotary system - Crown- down technique wiht Race rotary system After instrumentation, microbial cultures were taken from root canals and the reduction rate of bacteria were evaluated and compared using one way ANOVA test with P<0.05 as the limit of significance.

Results: There was no significant difference among the three instrumentation procedures regarding bacterial elimination.

Conclusion: According to the finding of this study, K-type manual file, Profile and Race rotary systems, all can be used in canal instrumentaion. However, since manual files are more accessible and require less equipment compared with rotary systems, and since the ability of all of these methods is identical regarding bacterial elimination, manual files can be used in straight canal instead of rotary systems.

M. Sharifian, B. Bolhari, A. Nosrat, M. Aligholi,
Volume 22, Issue 1 (8-2009)

Background and Aim: Researches have shown that bacteria play the main role in development of pulpal and periapical diseases. Chemo-mechanical cleaning of infected root-canal system can not remove all of the microorganisms. Thus interappointment medicaments are necessary to aid this goal. Calcium hydroxide is one of the most useful medicaments in root canal therapy, but this medicament can not eliminated all of the bacteria in root canal system. Carvacrol is an edible plant extract that has antimicrobial and anti-inflammatory effects. If this extract is effective against endodontic bacteria, it can be used as an root canal medicament.

Materials and Methods: In this experimental study, Initially, MIC and MBC of carvacrol detected with Macro broth dilution method and determined as 0.3% and 0.6%, respectively. After that, 30 single root and single canal extracted human teeth were used in this study. The number of specimens determined in a pilot study on 4 extracted teeth. After preparation to apical size # 30 with hand and rotary instruments, teeth were randomly divided into two experimental and two control groups. After culturing Enterococcus faecalis in prepared canals, we used emulsion of 0.6% carvacrol and calcium hydroxide in two A and B experimental groups for 7 days as the intracanal medicament. Microbial samples obtained before and after experiment. Then, canals with negative culture selected to obtain dentinal shaving to culture. Data obtained from microbiological samples analyzed with kruskal-wallis and Bonferroni tests.

Results: Results of this study showed that emulsion of 0.6% carvacrol has no significant difference with calcium hydroxide in elimination enterococcus faecalis after 7 days dressing (p>0.05).

Conclusion: Carvacrol can be used as an intrappointment intracanal medicament.

Mr. Sharifian, N. Shokouhinejad, Hr. Monsef Esfahani, M. Aligholi, M. Amjadi,
Volume 24, Issue 3 (6-2011)

Background and Aims: Instrumentation of the root canals results in formation of smear layer which covers the dentinal tubules. In infected teeth, it is ideal to achieve a material that has the ability to remove the smear layer besides antimicrobial activity. Therefore, this study was designed to evaluate the antimicrobial effect of Citrus aurantifolia extracts (lime juice and rind extract) on Enterococcus faecalis within dentinal tubules in the presence of smear layer.
Materials and Methods: One-hundred and forty dentin tubes were prepared from bovine incisors. After removal the smear layer, the specimens were infected with Enterococcus faecalis. Then, the smear layer was reformed. Test solutions were used as the irrigants in study roups as follows: group 1: 5.25% NaOCl group 2: 17% EDTA group 3: NaOCl+EDTA group 4: Lime juice group 5: ethanolic rind extract of C.aurantifolia group 6: 96% ethanol. Dentin chips were collected from inner and outer layers of dentinal walls and optical density was measured. The data were analyzed using one-way ANOVA and Tamhane tests.
Results: In outer layer of dentin, the efficacy of rind extract was less than that of NaOCl+EDTA (P<0.05). Also Lime juice was less effective than EDTA, NaOCl and NaOCl+EDTA (P<0.05). In inner layer of dentin, Lime juice was significantly less effective than NaOCl and NaOCl+EDTA (P<0.05). The efficacy of rind extract was less than that of NaOCl+EDTA (P<0.05).
Conclusion: In the presence of smear layer, the antimicrobial activity of Lime juice was less than that of NaOCl but the efficacy of rind extract was similar to that of NaOCl.

Mahdi Tabrizizadeh, Hengame Zandi, Maryam Kazemipoor, Fatemeh Mokhtari, Abbas Dehghani,
Volume 25, Issue 3 (7-2012)

Background and Aims: Calcium hydroxide has a pronounced antimicrobial activity against most of the bacterial species found in infected root canals and endodontic infections. It is one of the most frequently used intracanal medications in endodontic therapy. The objective of this study was to determine the antimicrobial efficacy of six calcium hydroxide formulations (mixed with saline, lidocaine 2%, chlorhexidine 2%, chlorhexidine 0.2%, Iodine Potassium iodide (IKI) 2%, and glycerin) on Enterococcus faecalis using agar diffusion test.
Materials and Methods: Twelve culture plates were incubated with Enterococcus faecalis. Five cavities were made in each plate with 5 mm diameter and 4 mm depth. Two plates were randomly considered for each calcium hydroxide formulation and filled completely with creamy mixture of tested materials. The plates were incubated at 37ºC for 48 hours. The diameter of inhibition zone around each well was recorded in millimetres and data were submitted to ANOVA and Tukey tests.
Results: The results of this study showed that all tested calcium hydroxide pastes had good antimicrobial activity. The antimicrobial activity of calcium hydroxide mixed with lidocaine, chlorhexidine 2% and IKI 2% were significantly greater than that of calcium hydroxide mixed with saline (P<0.001). No significant differences in the antimicrobial  were found between the other groups (P>0.01).
Conclusion: Considering the results of this study, lidocaine 2%, chlorhexidine 2% and IKI 2% are suggested to be used for preparation of calcium hydroxide paste. Further studies with different methods are needed for confirming these results.

Sharareh Mousavi Zahed, Kamal Amini, Jahromi Maryam Zare ,
Volume 28, Issue 1 (5-2015)

  Background and Aims: To ensure root canal treatment success, endodontic microbiota should be efficiently reduced. Several irrigation devices have been recently introduced with the main objective of improving root canal disinfection.The purpose of this study was to evaluate the rinsing effect of RinsEndo system in reduction of enterococcus faecalis in comparison with conventional hand syringe in infected root canals.

  Materials and Methods: 60 extracted single canal anterior teeth were infected with enterococcus faecalis and divided into 3 groups: RinsEndo system, conventional hand syringe and control group. The enterococcus faecalis colonies were counted in each group before and after rinsing. Data were analyzed using Variance and Kruskal Wallis test.

  Results: The mean of enterococcus faecalis growth after rinsing was 3.50×103 in group with conventional syring rinsing, 2.04×103 in group with RinsEndo washing and 6.11×103 in control group. Reduction of enterococcus faecalis after rinsing was statistically significant in each group (P<0.001). The amount of reduction in number of colonies with RinsEndo and conventional syringe rinsing was higher in comparison with control group and this difference was significant (P<0.001). RinsEndo rinsing effect was statistically significantly higher in comparison to conventional syringe as well (P<0.001).

  Conclusion: Rinsing with RinsEndo system was significantly more efficient in reduction of enterococcus faecalis from root canal in comparison with hand syringe washing.

Fatemeh Mokhtari, Amir Hossein Mansor Abadi, Ali Gharaati Jahromi, Ehsan Anvar, Zandi Hengame, Tabrizi Zade Mehdi, Taghavi Mayam,
Volume 28, Issue 3 (10-2015)

Background and Aims: Bacteria and their products have a major role in pulp and periapical diseases. The purpose of this study was to compare the antimicrobial activity of sodium hypochlorite, iodide potassium iodide and chlorhexidine on the enterococcus faecalis after root canal filling.

Materials and Methods: In this experimental study, 95 single canal human teeth were collected. Then after washing and cleaning and filing, the sterilization done using an autoclave. The teeth were smeared with enterococcus faecalis except five which selected as controls and then divided them into 3 groups which A randomly represented the irrigation by sodium hypochlorite, iodide potassium iodide (IKI) and chlorhexidine solution were done. The teeth were filled with gutta-percha and then incubated in a incubator for 90 days at a temperature of 37° C. The specimens were analyzed for Colony Count. Data were analyzed using Kruskal-Wallis test and Chi-Square.

Results: According to the results of this study, the correlation between the 3 groups of cultured teeth were not significant (P=0.812).The specimens which were washed by IKI had the most positive amount of cultures (23.3%) and the specimens which washed by chlorhexidine had the lowest (16.7%).

Conclusion: The results of this study showed that after the use of different wash solutions, no statistically significant difference exist in their antimicrobial activity after root canal therapy.

Fatemeh Mokhtari, Hengame Zandi, Asghar Nafez,
Volume 28, Issue 4 (1-2016)

Background and Aims: The presence of microbes inside the canal is the main reason for post-treatment infection. Therefore, the maintenance of the disinfection obtained during the treatment is imperative. The aim of this study was to compare the antimicrobial effect of Tricresol formalin and 5.25% Sodium hypochlorite on the Enterococcus faecalis.

Materials and Methods: In this study 66 human single-rooted extracted teeth were used. After access cavity and root canal preparation, the teeth were sterilized in autoclave, and then contaminated with Enterococcus faecalis suspension and incubated at 37°C for 7 days. Then, they were randomly divided into three groups of 20: In group 1, Tricresol formalin was used as intracanal medicament. In group 2, 5.25% Sodium hypochlorite and in group 3 (control group), normal saline were used as irrigants. 7 days after incubation at 37°C, the microbiological sampling was performed. For this purpose, dentinal shaves were collected from the root canals and cultured in Tryptic Soy Broth, and the number of Colony-Forming Unit (CFU) was counted. Data were analyzed using Kruskal-Wallis and Mann-Whitney tests.

Results: A significant reduction of CFU was observed in the Tricresol formalin and Sodium hypochlorite groups compared to the control group (P<0.001). No significant difference was reported between Tricresol formalin and Sodium hypochlorite groups (P=0.69).

Conclusion: The present study showed that the antimicrobial effect of Tricresol formalin was comparable with 5.25% Sodium hypochlorite on the Enterococcus faecalis.

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