Payavard Salamat

Satisfaction and opinions of Tehran University of Medical Sciences academic members on its development programs , 2006

SN Nejat

Background and Aim: Surveying faculty members can prove beneficial in the assessment of the university's performance in various domains, and the design and planning of suitable interventions. The current study assesses faculty members' opinions on the university's development programs.

Materials and Methods: One hundred and forty nine individuals completed the questionnaire in this cross-sectional study that was done through systematic sampling. The questionnaire covered demographic questions, professional specifications, faculty promotion criteria, sabbatical leaves, faculty participation in short-term educational programs, being in contact with graduates, recruiting foreign students, distant learning, processes involving research proposal approval, rewards and encouragement policies, faculty participation in health care management and distribution of budget among schools and hospitals.

Results: 71.8% of participants were male. The mean numbers of years of service in the university was 14.2 with a standard deviation of 7.5. Most participants were satisfied with the current status of promotion, processes involving research proposal approval and their participation in short-term educational programs. However most were not happy with the current status of sabbatical leaves.

Conclusion: Surveying through the methodical approach is a valuable tool in guiding senior university directors and its repetition and continuity can strengthen faculty participation and assess changing procedures.

Comparative study of evolution process and structure of systematized nomenclature of medicine in America , England and Australia countries

E Partovipoor

Background and Aim: Systematized Nomenclature of Medicine systems are the important supportive for electronic health record in registration and retrieval of data. Systematized Nomenclature of Medicine - Clinical Terms (SNOMED CT) is the most comprehensive language and then the consistency of exchanged data across health care providers and finally the high effectiveness of health care.

Materials and Methods : This research is a descriptive - comparative study and has been done in certain time section. Ideally , it have been used the descriptive - comparative method for studying of selective countries.

Results : Findings of this study show that American and England countries have accepted the SNOMED CT with application in all clinical activities and Australia country is evaluating this system and is using older versions of SNOMED only in pathologic applications now. Study shows that SNOMED CT structure is complex and based of concept (clinical concept) and contains two fundamental parts : 1) content of SNOMED CT core (concepts , descriptions and relations) that are always constant 2) content of out of SNOMED CT core (mappings or relation with systems and local extensions) that according to health and management needs and native terms of country are different. Such a structure executes in different software applications.

Conclusion : SNOMED CT structure in America and England countries has been compared and with attention to similarities (content of SNOMED CT core) and differences (content of out of SNOMED CT core : cross mappings and local extensions) , a basic SNOMED CT structure has been provided for Iran. Because of SNOMED CT in Australia is under development, researcher has studied its evolution and execution process in this country and has provided an appropriate structural pattern for Iran.

Information registration in surgical special sheets for discharege patients in Tehran University of Medical Sciences Teaching Hospitals , 2005

R Sharifian

Background and Aim: Currently in most hospitals medical mission and medical documents secession don't be notified about any disciplines in the framework of documenting medical data and in parallel the document makers will be overshadowed by non-executing these principles qualitatively and quantitatively(1).

Materials and Methods: The above study is a sectional one that describes the situation of recording informational items in the surgical special sheets. In order to collect information collect information from considered sheets, it has been used of 1040 files of hospitalized because of surgery operation in the under study hospitals.

Results: The results of the study showed that 67.5% and 53.4% of informational items have not been recorded in nte sheets General and special hospitals. In the operation report sheet more informational items has been recorded in proportion to others which amounts to 59.9%. Informational items related to the tests in the pre operation care sheet, complementary information in the operation report sheets, and after surgery care and observed side-effects in the anesthesia sheet have the most non recording information.

Conclusion: The situation of recording informational items in the surgical special sheets in contrast to previous studied samples is not desirable. Regarding to the importance of these sheets and their position in relation to the information recorded in them, it is necessary to take needed measures to remove the factors that result in non recording the informational items.

Detection of PLZF- RARa fusion in patients with Acute Promyelocytic Leukemia

SH Rostami

Background and Aim: Acute promyelocytic leukemia (APL) is associated with the t(1517) ,fusing promyelocytic leukemia (PML) and retinoic acid receptor-a (RARa) genes. This disease is uniquely sensitive to treatment with all-trans retinoic acid (ATRA) and highly responsive to conventional chemotherapy. The t(1117)(q23q21) abnormality associated with a PLZF-RARa rearrangement is the commonest of the alternative translocations accounting for less than 1% APL. Blasts from PLZF-RARa cases have been found to be resistant to the differentiating effects of retinoids. In this study we aimed to determine the PLZF-RARa and fusion genes in patients with APL morphology who referred to Hematology-Oncology and BMT research center, Tehran Shariaty Hospital in 2006.

Materials & Methods: Peripheral blood and/or bone marrow samples were taken from 200 patients with APL morphology and 200 patients with other subtypes of AML. The mono-nuclear cells were enriched by centrifugation over a ficoll-isopaque gradient. RNA extracted by Trizol or TriReagent and then reverse transcribed to cDNA using random primers. PCR performed using specific primers for each fusion. PCR products electerophoresed on a 2% agarose gel containing 0.05% ethidiume bromide.

Results: In 2 (1%) patients with APL-morphology the RT-PCR analysis showed PLZF-RARa fusion transcripts.

Conclusion: It can be concluded that RT-PCR is a rapid and sensitive method for detection of abnormal fusion genes in leukemia and allows the definition of a correct strategy for treatment and subsequent minimal residual disease monitoring

Precompetetive cardiovascular screening in elderly wrestlers

V Ziaee

Background and Aim: Sudden death in athletes commonly is due to unknown cardiovascular diseases. Therefore many protocols have designed for screening in the world. In the last years has been spent more attention to sport in elderly athletes and the number of their competitions have been increased. The aim of this study was evaluation of risk factor of cardiovascular diseases in Iranian elderly wrestlers.

Materials and Methods: All the members (60 athletes) of Iran team in Elderly Wrestling World Championship 2006 have taken census in the present study. At two sections the cardiovascular health state of them has evaluated: history taking and physical examination, electrocardiography and exercise test. All date was analyzed by SPSS version 14 and mean and standard deviation for presenting findings.

Results: Sixty athletes aged 37 to 78 years (54.65±8.75) with history of exercise from 12 to 55 years (37.8±9.4) have been studied. In the history of 66.7% and in physical examination and history 75.1% athletes had at least one risk factor. Exercise test results of wrestlers were highly positive and positive in 8.6% and 5.2% respectively.

Conclusion: We suggest a perfect history taking and a complete physical examination for each athlete at the first step. If you found abnormalities then noninvasive diagnostic testes such as electrocardiography, exercise test and echocardiography will be useful and informative.

Study of concentration of added colors to juice of black cherry and juice of barberry on display in shop in the city of Tehran

MM Soltan Dallal

Background and Aim: Foodstuffs additives are a general term for materials that are added to the foodstuffs in order to increase durability and to improve the appearance, composition, taste and food nutritional value. Colors are classified in this group and are added to foodstuffs in order to increase their attraction. The aim of this survey was to analyze status of added colors to the juice of black cherry and juice of barberry which are produced in Tehran City, capital of Iran.

Materials and Methods: Three haundred thirty six samples of dried sweets were randomly collected and analyzed from different areas of Tehran. First, the samples were de-colored by Clorhidric Acid, and then were analyzed after refining by Thin Layer Chromatography (T.L.C) method.

Results: Eighty nine percent from the total samples contained colors. Among chromatic samples, 62 samples (18.5%) out the total samples, contained artificial, non-edible colors and 237 samples (70.5%) from the total samples contained artificial and edible colors (for Industrial Producers) and 37 samples (11%), contained natural colors. Carmoisine color was detected more than added colors in juice of black cherry and juice of barberry.

Conclusion: Low costs, stability, Ph and similarity of artificial dyes with natural dyes motivate the producers for high utilization of these dyes without considering their possible hazards and/or their edible quality aspects.

Conflict Management and its related strategies

H Dargahi

Conflict management is a kind of management which could be administered the organization with the best ways and create balance between organization and staffs and eliminates the conflict. Generally , conflict management is the process of conflict roles recognition between intergroups and intragroups and use of conflict techniques for eliminate or simulate conflict for organizational effectiveness.
This research is aimed to introduce conflict management and how to use its strategies to modify this phenomenon.
This research shows that organizational conflict have both instructive and destructive results. Managers should use conflict management in every organization. Most conflict management strategy used to control conflict is collaboration between managers and staffs. Conflict management has important role to make managers successful . Although, a minimum of conflict is appropriate in every organization but no managers agree with anarchism in his organization

Human Telomerase Reverse Transcriptase (hTERT) expression in patients with Chronic Myelogenous Leukemia at defferent phases of the disease

Y Mortazavi

Background and Aim: Chronic Myelogenous Leukemia (CML) is a clonal hematopoietic stem cell disorder characterized by a translocation between chromosome 9 and 22 called Philadelphia Chromosome. Telomerase- essential enzyme that adds telomeric repeats into the telomeres- maintains integrity of chromosomal ends. Most normal somatic cells do not express hTERT (catalytic subunit of telomerase) but most neoplasia and cancer cells express it. In this study we evaluated the hTERT expression in patients with CML at different phases of the disease.

Materials and Methods: In this cross sectional study, 73 samples of 45 patients with CML were studied. Twenty six of samples were taken from patients in chronic phase before therapy and 26 samples three month after therapy. Nine samples were taken in accelerated phase and 12 in blastic phase. hTERT expression was studied by RT-PCR and the results were analyzed using SPSS 15.

Results: Thirty three (73%) of patients were male and 12 (23%) were female. Patients were divided into three age groups 17-29, 30-40 and 41-75 years. Of 73 samples, 43 samples (58.9%) were positive for hTERT and 30 samples (41.1%) were negative for this gene. In chronic phase (before therapy) 69.2% of the samples were PCR positive, but after therapy only 38.5% of samples were PCR positive. In accelerated and blastic phases, 55.6% and 83.3% of samples were PCR positive respectively. The hTERT positivity was differently significant (p<0.05) among different phases of the disease.

Conclusion: Significant difference between hTERT expression in different phases of CML disease can be used as a useful molecular marker for fallowing up, prognosis and disease progression after treatment

Assessment of TPS tumor marker with ELISA for early detection and monitoring of Breast Cancer

AR Salehi Nodeh

Background and Aim: Fatal disease of cancer, ranks second after cardiovascular disease, as the most important causes of mortality in many developing countries, including Iran. Breast cancer is the most common disease among women. TPS is one of the tumor markers which has specially been considered due to its exclusive physiological characteristics like its easy measurement in serum of cancer patients.

Materials and Methods: This study has been set up to evaluate the efficiency of this tumor marker in the prognosis, treatment control and follow up of patients with Breast cancer. TPS has been measured with ELISA in 34 persons including 28 healthy people and 6 patients with breast cancer. Sampling has been done in three times depending on treatment methods.

Results: TPS has been measured in samples which contend of 2 to 3 ml of serum from patients and the health.

Conclusion: The results has revealed that the serum TPS is not only as a measure of prognosis but also would be helpful in follow up and treatment control of the disease.

Conclusion: Serological analysis can be settled in the diagnosis and trend of cancer with production of polyclonal antibody against TPS gene family and planning appropriate pattern.

Investigation of P16INK4A and P14RF genes expression in different phase of Chronic Myeloid Leukemia (CML)

SH Ghaffari

Background & Aim : Chronic myeloid leukemia (CML) is a disorder of pluripotential hematopoietic stem cell that is as a myeloproliferative disease and occurs in about 15 percent of all leukemia. Two cell cycle regulatory proteins that function as tumor suppressor are P16INK4A and P14ARF. The origin of these two proteins is a human INK4A-ARF gene locus that located on chromosome 9p21. P16INK4A control retinoblastoma (Rb) and P14ARF control with p53 thought negative feedback. The purposes of this study, this was that whether these genes are preferable use as a factor in prognosis and progression of disease.

Materials and Methods: This research was a Cross sectional study. The expression of p16INK4A and p14ARF mRNA in about 73 peripheral bloods (PB) Samples were collected from 45 CML patients at different phases of disease were assayed by reverse transcriptase polymerase chain reaction (RT-PCR). 26 samples were from patients at chronic phase before any treatment, 26 samples 3 month after treatment with imatinib, 9 samples in accelerated phase and 12 samples in Blastic phase.

Results. From 45 patients with CML, 33 patients (73%) were men and 12 patients (27%) were women. About 26 samples (35%) were p16INK4A positive and 55 samples (75%) were p14ARF RT-PCR positive. This expression of the two genes at different phases of disease were not statistically significant (p>0.05).

Conclusion: High percentage of the CML patients expressed P14ARF and P16INK4A genes. The expression of these gene at different phases of disease (diagnosis, accelerate, and Blastic phases) was not statistically significant even though, the expression of these genes was higher after the treatment. The increased expression of these genes was probably because of the Imatinib treatment.

Evaluation of VDRL test in Patients Rheumatoid arthritis

AR Salehi Nodeh

Background and Aim: Rheumatoid arthritis is a systemic, chronic and inflammatory disease. It is characterized by the symmetric synovitis of the articules of the extremities. Its major cause is unknown.
Immunological factors including Rheumatoid factor (RF) are considered as more confirming Cause. To date Rheumatoid factor is consisted of many antibodies which are produced against the constant region of IgG. Its causes are not known. This factor is not detected in the healthy people. In the patients with positive rheumatoid factor the probability of a disease would increase seriously. The goal of this study is evaluation of VDRL test in rheumatoid arthritis.

Materials and Methods : Serum Samples were obtained from 70 patients with rheumatoid arthritis from loghman hospital. We selected 70 serum samples from the blood donating healthy people.
The serum samples were kept in the Capped vials in - 20ºc freezer. Within two week, VDRL serologic tests were done on serum samples.

Results : The patients were 49 females and 21 males. In the female group 79.6% had negative response and 20.4% had positive response.
In the male patients 66.7% had negative response and 33.3% had positive response. Positive tests were much more in males. In healthy people only 4 ones has positive VDRL test.(5.7%), versus 17 positive VDRL test in patient group (25.3%). In the normal group, 94.3% had negative test versus 75.7% in the patient group.

Discussion : The difference between two groups was statistically Significant (p<0.01). The result of this study shows that VDRL test would be helpful in the diagnosis of rheumatoid arthritis.

Study of Sick Building Syndrome (SBS) prevalence in hospitals and other health care centers employees

E Jazayeri Gharebagh

Background & Aim : Symptoms associated with acute discomfort, e.g., headache eye, nose, or throat irritation dry and itchy skin, which are known as Sick Building Syndrome (SBS) are well reported amongst people who work in hospital environment especially in the X- ray department. The aim of this study was to determine the prevalence of the symptoms and to identify work related factors in hospital environment.

Materials and Methods : A questionnaire survey has been done from 219 hospital personnel who worked in the university hospitals (medical X-ray technologists, physiotherapists and office staff) as the exposed group and 219 personnel of the same occupations from private health care units and offices as the control group about if they experienced any of the mentioned symptoms in the last eight weeks. A total of 215 and 216 the questionnaire were accepted from each group respectively and included in analyses.

Results: From data provided by this work, 6 symptoms (headache, sorethroat, sinusitis, dermatitis,nasalaids and sore eyes) have significant relationship between exposed and control groups. One symptom (catarrh) had significant difference among physiotherapists (P< 0.05), but with no significant difference between hospital and non-hospital environment. In addition, there was a relationship between incidence of dermatitis and history of skin allergy (P< 0.05).

Conclusion: The results from this preliminary work are similar with the studies, which have been done by others. These results also suggest that there is a link between incidence of SBS and hospital based chemical agents.