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This research is a descriptive study which had been preformed to identity theknowledge , Attitude and practice of the health Workers of south area inTehran and Quom about prevention of malaria disease in 1995. the sampleswere consist of 50 health workers, who had been selected by a class samplingmethod from a targer population . The data collection instruments were a questionnair and a check list.The questionnair had four separate sections as follow :l)The demographic with 8 questions2) The knowledge with 20 multiple choice questions3)The attitude section with likert type questions4) The evaluative Section of practice with 8 questions in order to analysis the data, it was used discriptive and deductive type of statistics.The information has been summerized in 17 tables and 11 graphs .The results Showed that: most of the subjects have had moderate knowledgeand practice about the prevention of malaria. a negative attitude showd thatthere is also asignificantrelationshipamongknowledge and practice withhaving affected patients also there is no significant relationship betweendemographic data and their attitudes Analysis of the data , it indicated a significant relation ship between knowldge and attiluds knowledge practice,also attitude and practice of health workers.At the end application of the results, findings and the suggestion, were madefor the future resercher,