Volume 19, Issue 1 (1-2020)
ijdld 2020, 19(1): 44-50 |
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Moradi Z, Ravari M S, Farrokhi E, Hashemzadeh Chaleshtori M. INVESTIGATION OF METHYLATION OF TNF-Α GENE PROMOTER IN PATIENTS WITH TYPE 2 DIABETES. ijdld 2020; 19 (1) :44-50
URL: http://ijdld.tums.ac.ir/article-1-5866-en.html
URL: http://ijdld.tums.ac.ir/article-1-5866-en.html
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2- Department of Molecular Medicine, School of Advanced Technologies, Shahrekord University of Medical Sciences, Shahrekord, Iran ,e_farrokhi_k@yahoo.com
3- Modeling in Health Center, Shahrekord University of Medical Sciences, Shahrekord, Iran
2- Department of Molecular Medicine, School of Advanced Technologies, Shahrekord University of Medical Sciences, Shahrekord, Iran ,
3- Modeling in Health Center, Shahrekord University of Medical Sciences, Shahrekord, Iran
Abstract: (2785 Views)
Background: Type II diabetes is a chronic inflammatory condition that is associated with a combination of genetic and environmental factors. Tumor necrosis factor alpha or TNF-α as an adipocyte cytokine, which affects the signaling pathway of insulin, can contribute to insulin resistance in type 2 diabetes patients. Considering the importance of epigenetic changes in multifactorial diseases, this study aimed to investigate TNF-α promoter methylation in patients with type 2 diabetes.
Methods: This study was performed on 61 patients with type 2 diabetes and 31 non-diabetic patients. The Groups were matched in terms of demographic characteristics. The lipid profiles were measured by standard kits. TNF-α promoter methylation levels were measured by bisulphite treatment method, Nested PCR and sequencing.
Results: There was no association between TNF-α promoter methylation gene promoter and type 2 diabetes in the studied groups. Also, there was no association between TNF-α promoter methylation in diabetic and non-diabetic groups between males and females.
Conclusion: The epigenetic changes in cytokines that contribute to insulin resistance in type 2 diabetic patients seem to be ineffective in peripheral blood samples, and other tissues may need to be investigated in this regard.
Methods: This study was performed on 61 patients with type 2 diabetes and 31 non-diabetic patients. The Groups were matched in terms of demographic characteristics. The lipid profiles were measured by standard kits. TNF-α promoter methylation levels were measured by bisulphite treatment method, Nested PCR and sequencing.
Results: There was no association between TNF-α promoter methylation gene promoter and type 2 diabetes in the studied groups. Also, there was no association between TNF-α promoter methylation in diabetic and non-diabetic groups between males and females.
Conclusion: The epigenetic changes in cytokines that contribute to insulin resistance in type 2 diabetic patients seem to be ineffective in peripheral blood samples, and other tissues may need to be investigated in this regard.
Type of Study: Research |
Subject:
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Received: 2019/06/23 | Accepted: 2020/01/21 | Published: 2019/11/1
Received: 2019/06/23 | Accepted: 2020/01/21 | Published: 2019/11/1
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