Volume 18, Issue 4 (4-2019)
ijdld 2019, 18(4): 189-198 |
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Rezaei Nasab H, Habibi A, Nikbakht M, Rashno M, Shakerian S. THE EFECT OF DICHLOROACETATE AND ENDURANCE TRAINING ON THE GENES EXPRESSION OF SUPEROXIDE DISMUTASE (SOD) AND GLUTATHIONE PEROXIDASE (GPX) IN CARDIAC MUSCLE OF DIABETIC MALE RATS
. ijdld 2019; 18 (4) :189-198
URL: http://ijdld.tums.ac.ir/article-1-5875-en.html
URL: http://ijdld.tums.ac.ir/article-1-5875-en.html
1- Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, Shahid Chamran University, Ahvaz, Iran , hamed.rezai93@yahoo.com
2- Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, Shahid Chamran University, Ahvaz, Iran
3- Department of Immunology, School of Medicine, Ahvaz Jundishapur University of Medical sciences, Ahvaz, Iran
2- Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, Shahid Chamran University, Ahvaz, Iran
3- Department of Immunology, School of Medicine, Ahvaz Jundishapur University of Medical sciences, Ahvaz, Iran
Abstract: (3302 Views)
Background: Oxidative stress plays a key role in the onset and development of diabetes Complications, Including diabetic cardiomyopathy. The purpose of this study was to investigate the role of dichloroacetate (DCA) on SOD and GPX expression following six weeks’ endurance training in cardiac muscle of diabetic male rats.
Methods: In this experimental study, 64 male Wistar rats were selected and randomly divided into eight groups after streptozotocin (STZ) solution diabetic treatment. The endurance training protocol was performed on a treadmill for 6 weeks. In the present study, for Inhibition of PDK4 in the cardiac muscle, intraperitoneal injection of DCA of 50 mg/ kg body weight was used. Gene expressions were measured by Real-Time PCR method. One-way ANOVA and Tukey's test were used to analyze the data.
Results: The results of the study showed that after endurance training, PDK4 gene expression increased and SOD and GPX genes expression in training endurance + diabetic group and endurance training group decreased compared to control group (P <0.05). By Inhibition of PDK4, the of SOD and GPX genes expression increased in DCA + training endurance + diabetic group and DCA + endurance training group compared to control group (P <0.05).
Conclusion: According to the results of this study, DCA injections may reduce the recurrence of free radicals induced by endurance training in diabetic patients by mitochondrial adaptation. Which can reduce the oxidative stress in the heart tissue of diabetic patients and increase cardiac efficiency.
Methods: In this experimental study, 64 male Wistar rats were selected and randomly divided into eight groups after streptozotocin (STZ) solution diabetic treatment. The endurance training protocol was performed on a treadmill for 6 weeks. In the present study, for Inhibition of PDK4 in the cardiac muscle, intraperitoneal injection of DCA of 50 mg/ kg body weight was used. Gene expressions were measured by Real-Time PCR method. One-way ANOVA and Tukey's test were used to analyze the data.
Results: The results of the study showed that after endurance training, PDK4 gene expression increased and SOD and GPX genes expression in training endurance + diabetic group and endurance training group decreased compared to control group (P <0.05). By Inhibition of PDK4, the of SOD and GPX genes expression increased in DCA + training endurance + diabetic group and DCA + endurance training group compared to control group (P <0.05).
Conclusion: According to the results of this study, DCA injections may reduce the recurrence of free radicals induced by endurance training in diabetic patients by mitochondrial adaptation. Which can reduce the oxidative stress in the heart tissue of diabetic patients and increase cardiac efficiency.
Type of Study: Research |
Subject:
Special
Received: 2019/07/13 | Accepted: 2019/09/5 | Published: 2019/04/15
Received: 2019/07/13 | Accepted: 2019/09/5 | Published: 2019/04/15
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