Volume 19, Issue 5 (6-2020)
ijdld 2020, 19(5): 234-251 |
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Khoshnevisan K, Sajjadi-Jazi S M, Faridbod F, Larijani B, Khorramizadeh M R. DESIGN AND FABRICATION OF ELECTROCHEMICAL BIOSENSOR BASED ON NANO-GRAPHENE TO DETECT SEROTONIN IN DIABETIC ZEBRA FISH. ijdld 2020; 19 (5) :234-251
URL: http://ijdld.tums.ac.ir/article-1-5968-en.html
URL: http://ijdld.tums.ac.ir/article-1-5968-en.html
Kamyar Khoshnevisan1 
, Sayed Mahmoud Sajjadi-Jazi2 , Farnoush Faridbod3 , Bagher Larijani2 , Mohammad Reza Khorramizadeh * 4
, Sayed Mahmoud Sajjadi-Jazi2 , Farnoush Faridbod3 , Bagher Larijani2 , Mohammad Reza Khorramizadeh * 4
1- Biosensor Research Center, Endocrinology& Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran
2- Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran
3- School of Chemistry, College of Science, University of Tehran, Tehran, Iran
4- Biosensor Research Center, Endocrinology& Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran ,khoramza@tums.ac.ir
2- Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran
3- School of Chemistry, College of Science, University of Tehran, Tehran, Iran
4- Biosensor Research Center, Endocrinology& Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran ,
Abstract: (1976 Views)
Background: A novel nanocomposite-modified electrode based on reduced graphene oxide (rGO) decorated with crown-ether and gold nanoparticles (GNPs) on the surface of a glassy carbon electrode (GCE) was fabricated to investigate 5-HT determination.
Methods: The morphology of nanocomposite was characterized by scaning electron microscopy (SEM). Diabetic zebrafish was obtained by overfeeding via glucose. 5-HT was successfully determined in the presence of dopamine (DA), ascorbic acid (AA), urea, glucose, and L-tryptophan (L-Trp) by using electrochemical methods.
Results: The nanocomposite exhibited satisfactory electrochemical catalytic activity for 5-HT determination using square-wave voltammetry (SWV). The electrochemical behavior of 5-HT at the nanocomposite/GCE displayed reasonable oxidation current and potential. The limit of detection (LOD) of 5-HT obtained from the real samples, containing the control and diabetic group by using the proposed system and HPLC method, was calculated to be about 0.3 and 0.1 µg/L, respectively. The prposed system also demonstrated high selectivity, reasonable sensitivity, and good stability and reproducibility for 5-HT sensing. The nanocomposite was applied for the determination of the biomarker 5-HT in the diabetic and control groups of zebrafish and displayed excellent recoveries about 93 and low relative error about 3% while compared with standard method.
Conclusions: It seems that the 5-HT level can be used for earlier diagnosis of diabetes.
Methods: The morphology of nanocomposite was characterized by scaning electron microscopy (SEM). Diabetic zebrafish was obtained by overfeeding via glucose. 5-HT was successfully determined in the presence of dopamine (DA), ascorbic acid (AA), urea, glucose, and L-tryptophan (L-Trp) by using electrochemical methods.
Results: The nanocomposite exhibited satisfactory electrochemical catalytic activity for 5-HT determination using square-wave voltammetry (SWV). The electrochemical behavior of 5-HT at the nanocomposite/GCE displayed reasonable oxidation current and potential. The limit of detection (LOD) of 5-HT obtained from the real samples, containing the control and diabetic group by using the proposed system and HPLC method, was calculated to be about 0.3 and 0.1 µg/L, respectively. The prposed system also demonstrated high selectivity, reasonable sensitivity, and good stability and reproducibility for 5-HT sensing. The nanocomposite was applied for the determination of the biomarker 5-HT in the diabetic and control groups of zebrafish and displayed excellent recoveries about 93 and low relative error about 3% while compared with standard method.
Conclusions: It seems that the 5-HT level can be used for earlier diagnosis of diabetes.
Type of Study: Research |
Subject:
Special
Received: 2020/07/18 | Accepted: 2020/10/28 | Published: 2020/06/30
Received: 2020/07/18 | Accepted: 2020/10/28 | Published: 2020/06/30
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