Volume 22, Issue 4 (11-2022)
ijdld 2022, 22(4): 199-212 |
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Badri Z, Delfan M, Danesh yar S. The Combined Effect of High-Intensity Interval Training and Metformin on Gene Expression of Myogenin and Myostatin in Skeletal Muscle of Type 2 Diabetic Mice. ijdld 2022; 22 (4) :199-212
URL: http://ijdld.tums.ac.ir/article-1-6173-en.html
URL: http://ijdld.tums.ac.ir/article-1-6173-en.html
1- Department of Exercise Physiology, Faculty of Sport Sciences, Alzahra University,Tehran, Iran
2- Department of Exercise Physiology, Faculty of Sport Sciences, Alzahra University,Tehran, Iran ,m.delfan@alzahra.ac.ir
3- Department of Physical Education, Faculty of Human Science, Ayatollahozmah Broujerdi University, Lorestan, Iran
2- Department of Exercise Physiology, Faculty of Sport Sciences, Alzahra University,Tehran, Iran ,
3- Department of Physical Education, Faculty of Human Science, Ayatollahozmah Broujerdi University, Lorestan, Iran
Abstract: (1097 Views)
Background: Myogenin (MyoG) and Myostatin (Mstn) play role in muscle growth and wasting, respectively. The present study aimed to investigate the combined effect of High-intensity Interval Training (HIIT) and Metformin drug (Metf) on gene expression of MyoG and Mstn in skeletal muscle of type 2 diabetic mice.
Methods: 25 mice (C57BL/6) were assigned to two groups, including 1) Control © (n=5), and 2) HFD (n=20). The mice of the HFD group were fed a high-fat diet for 16 weeks. After 16 weeks, the mice with over 200 mg/dl were selected as diabetic mice. Then, the diabetic mice were divided into four groups including 1) Control Diabetic (CD) (n=5), 2) Diabet with Metf (DM) (n=5), 3) Diabet with HIIT (DH) (n=5) 4) Diabet with Metf and HIIT (DMH) (n=5). The mice of experimental groups underwent the interventions for eight weeks. The Real-Time–PCR methods were used to measure the mRNA expression of MyoG and Mstn in the Rectus-Femoris muscle.
Results: HIIT (but no Metf) upregulated the gene expression of MyoG (p=0.05). Metformin did not affect Mstn expression (p=0.45), However, HIIT downregulated the expression of Mstn (p=0.001). Metformin did not affect decreasingly or incrementally the downregulating effect of HIIT on Mstn expression (p=0.95).
Conclusion: Metf may not positively or negatively affect the expression changes of MyoG and Mstn induced by HIIT in skeletal muscle of mice with type 2 diabetes.
Methods: 25 mice (C57BL/6) were assigned to two groups, including 1) Control © (n=5), and 2) HFD (n=20). The mice of the HFD group were fed a high-fat diet for 16 weeks. After 16 weeks, the mice with over 200 mg/dl were selected as diabetic mice. Then, the diabetic mice were divided into four groups including 1) Control Diabetic (CD) (n=5), 2) Diabet with Metf (DM) (n=5), 3) Diabet with HIIT (DH) (n=5) 4) Diabet with Metf and HIIT (DMH) (n=5). The mice of experimental groups underwent the interventions for eight weeks. The Real-Time–PCR methods were used to measure the mRNA expression of MyoG and Mstn in the Rectus-Femoris muscle.
Results: HIIT (but no Metf) upregulated the gene expression of MyoG (p=0.05). Metformin did not affect Mstn expression (p=0.45), However, HIIT downregulated the expression of Mstn (p=0.001). Metformin did not affect decreasingly or incrementally the downregulating effect of HIIT on Mstn expression (p=0.95).
Conclusion: Metf may not positively or negatively affect the expression changes of MyoG and Mstn induced by HIIT in skeletal muscle of mice with type 2 diabetes.
Type of Study: Research |
Subject:
Special
Received: 2022/08/20 | Accepted: 2022/10/25 | Published: 2022/11/1
Received: 2022/08/20 | Accepted: 2022/10/25 | Published: 2022/11/1
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