Volume 23, Issue 4 (11-2023)
ijdld 2023, 23(4): 245-255 |
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Sabaghian F, Kazeminasab F, Ghanbarpour A. The Effect of Aerobic Exercise on the Function of Local Renin-Angiotensin System and Insulin Resistance in the Gastrocnemius Muscle of Type 2 Diabetic Mice. ijdld 2023; 23 (4) :245-255
URL: http://ijdld.tums.ac.ir/article-1-6242-en.html
URL: http://ijdld.tums.ac.ir/article-1-6242-en.html
1- Department of Physical Education and Sport Sciences/Faculty of Humanities, University of Kashan, Kashan, Iran
2- Department of Physical Education and Sport Sciences/Faculty of Humanities, University of Kashan, Kashan, Iran ,f_kazemi85@yahoo.com
2- Department of Physical Education and Sport Sciences/Faculty of Humanities, University of Kashan, Kashan, Iran ,
Abstract: (732 Views)
Background: The purpose of this study was to investigate the impact of aerobic exercise on the function of local renin-angiotensin system and insulin resistance in the Gastrocnemius muscle of type 2 diabetic mice.
Methods: The number of 18 male C57BL/6 mice were divided into two groups: 1. Mice fed with a standard diet for 12 weeks (control group), 2. Mice fed with a high-fat diet for 12 weeks with streptozotocin injection (diabetes group). Diabetic mice were randomly divided into two groups: 1. Diabetes-Sedentary group, 2. Diabetes-exercise group. The mice of the training group exercised on the treadmill for eight weeks and five days/week with an average speed of 19 m/min and for 45 minutes. 24 hours after the last training session, the mice were sacrificed. The Gastrocnemius muscle tissue was extracted and stored in -80°C freezer to measure the expression level of angiotensin 2, Mass receptor, At1 receptor and glucose transporter 4 (Glut 4).
Results: The results showed that the expression of Mass and Glut4 receptor in diabetes-exercise mice was significantly higher than diabetes-sedentary group. Also, the expression of angiotensin 2, HOMA-IR, and fasting glucose in the muscle of diabetes-exercise mice were significantly lower vs. diabetes-sedentary mice. Also, the expression of At1 receptor in the diabetes-exercise group was insignificantly lower than in the diabetes-sedentary group.
Conclusion: The findings of the present study show the important role of exercise in improving the local renin-angiotensin system in skeletal muscle. Eight weeks of aerobic training can reduce muscular insulin resistance and improve type 2 diabetes by decreasing the expression of angiotensin 2, At1 receptor and increasing the Mass receptor.
Methods: The number of 18 male C57BL/6 mice were divided into two groups: 1. Mice fed with a standard diet for 12 weeks (control group), 2. Mice fed with a high-fat diet for 12 weeks with streptozotocin injection (diabetes group). Diabetic mice were randomly divided into two groups: 1. Diabetes-Sedentary group, 2. Diabetes-exercise group. The mice of the training group exercised on the treadmill for eight weeks and five days/week with an average speed of 19 m/min and for 45 minutes. 24 hours after the last training session, the mice were sacrificed. The Gastrocnemius muscle tissue was extracted and stored in -80°C freezer to measure the expression level of angiotensin 2, Mass receptor, At1 receptor and glucose transporter 4 (Glut 4).
Results: The results showed that the expression of Mass and Glut4 receptor in diabetes-exercise mice was significantly higher than diabetes-sedentary group. Also, the expression of angiotensin 2, HOMA-IR, and fasting glucose in the muscle of diabetes-exercise mice were significantly lower vs. diabetes-sedentary mice. Also, the expression of At1 receptor in the diabetes-exercise group was insignificantly lower than in the diabetes-sedentary group.
Conclusion: The findings of the present study show the important role of exercise in improving the local renin-angiotensin system in skeletal muscle. Eight weeks of aerobic training can reduce muscular insulin resistance and improve type 2 diabetes by decreasing the expression of angiotensin 2, At1 receptor and increasing the Mass receptor.
Keywords: Aerobic Exercise, Renin-Angiotensin System, Insulin Resistance, Gastrocnemius Muscle, Type 2 Diabetes
Type of Study: Research |
Subject:
Special
Received: 2023/06/3 | Accepted: 2023/07/23 | Published: 2023/11/1
Received: 2023/06/3 | Accepted: 2023/07/23 | Published: 2023/11/1
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