Volume 24, Issue 4 (10-2024)
ijdld 2024, 24(4): 232-244 |
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Rahmani L, Rahimi M R, Ahmadi S, Faraji H. The Effect of Eight Weeks Aerobic Training and Vitamin D3 Supplementation on Glut4 Protein Concentration and Insulin Resistance in the Skeletal Muscle of Diabetic Rats. ijdld 2024; 24 (4) :232-244
URL: http://ijdld.tums.ac.ir/article-1-6318-en.html
URL: http://ijdld.tums.ac.ir/article-1-6318-en.html
1- Department of Exercise Physiology, Faculty of Humanities and Social Sciences, University of Kurdistan, Sanandaj, Iran
2- Department of Exercise Physiology, Faculty of Humanities and Social Sciences, University of Kurdistan, Sanandaj, Iran ,r.rahimi@uok.ac.ir
3- Department of Biological Science, Faculty of Science, University of Kurdistan, Sanandaj, Iran
4- Department of Physical Education, Islamic Azad University, Marivan Branch, Marivan, Iran
2- Department of Exercise Physiology, Faculty of Humanities and Social Sciences, University of Kurdistan, Sanandaj, Iran ,
3- Department of Biological Science, Faculty of Science, University of Kurdistan, Sanandaj, Iran
4- Department of Physical Education, Islamic Azad University, Marivan Branch, Marivan, Iran
Abstract: (574 Views)
Background: Glucose transporter4 (GLUT4) is the main glucose transporter in skeletal muscle. Impaired GLUT4 expression plays a role in the disorders of glycemic homeostasis. The aim of the present study was to investigate the combined effects of aerobic training and vitamin D3 supplementation on Glut4 protein levels and insulin resistance in the soleus muscle of diabetic rats with STZ and high-fat diet.
Methods: In 40 male Wistar rats type 2 diabetes was induced by 6 weeks high-fat diet followed by streptozotocin injection. Then rats were randomly divided into five groups: Healthy control (HC), Diabetic control (DC). Diabetes+Aerobic training (DAT), Diabetes+Vitamin D3 (DVD) and Diabetes+Aerobic training+ Vitamin D3 (DVDAT). The rats underwent eight weeks of aerobic training and vitamin D3 supplementation. 24h after last session of training and, the rats were anesthetized and soleus muscle was isolated for measurement of Glut4 protein concentrations and serum levels of insulin, glucose, vitamin D3 index were measured.
Results: One-way ANOVA showed that GLUT4 protein levels in DC group was significantly lower than HC group (P<0.001), but in DVDAT group was significantly higher than DC group (P<0.04) and DVD group (P<0.005). Also in DAT group was significantly higher than DVD (P<0.018). The HOMA-IR index also in DVDAT, DAT and DVD groups was significantly lower than DC group (P<0.001).
Conclusion: It seems that Eight weeks of aerobic training with vitamin D3 supplementation improves glucose metabolism in diabetic rats via increasing Glut4 protein levels and improving insulin resistance index.
Methods: In 40 male Wistar rats type 2 diabetes was induced by 6 weeks high-fat diet followed by streptozotocin injection. Then rats were randomly divided into five groups: Healthy control (HC), Diabetic control (DC). Diabetes+Aerobic training (DAT), Diabetes+Vitamin D3 (DVD) and Diabetes+Aerobic training+ Vitamin D3 (DVDAT). The rats underwent eight weeks of aerobic training and vitamin D3 supplementation. 24h after last session of training and, the rats were anesthetized and soleus muscle was isolated for measurement of Glut4 protein concentrations and serum levels of insulin, glucose, vitamin D3 index were measured.
Results: One-way ANOVA showed that GLUT4 protein levels in DC group was significantly lower than HC group (P<0.001), but in DVDAT group was significantly higher than DC group (P<0.04) and DVD group (P<0.005). Also in DAT group was significantly higher than DVD (P<0.018). The HOMA-IR index also in DVDAT, DAT and DVD groups was significantly lower than DC group (P<0.001).
Conclusion: It seems that Eight weeks of aerobic training with vitamin D3 supplementation improves glucose metabolism in diabetic rats via increasing Glut4 protein levels and improving insulin resistance index.
Type of Study: Research |
Subject:
Special
Received: 2023/12/16 | Accepted: 2024/03/10 | Published: 2024/10/31
Received: 2023/12/16 | Accepted: 2024/03/10 | Published: 2024/10/31
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