Volume 24, Issue 4 (10-2024)
ijdld 2024, 24(4): 222-231 |
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Golmohammadi samani Y, Nazarali P, Alizadeh R, Rezaeinezhad N. The Effects of Eight Weeks of High-Intensity Interval Training (HIIT) on the Nrf2 Gene Expression, Lipid Peroxidation and Insulin Resistance in Pancreas Tissue of Aged Rats Fed a High-Fat Diet. ijdld 2024; 24 (4) :222-231
URL: http://ijdld.tums.ac.ir/article-1-6345-en.html
URL: http://ijdld.tums.ac.ir/article-1-6345-en.html
1- Department of Exercise Physiology, Faculty of Sports Sciences, Alzahra University, Tehran, Iran
2- Department of Sports Science, School of Literature and Humanities, Ilam University, Ilam, Iran ,r.alizadeh@ilam.ac.ir
3- Department of Exercise Physiology, Faculty of Sport Science and Health, University of Tehran, Tehran, Iran
2- Department of Sports Science, School of Literature and Humanities, Ilam University, Ilam, Iran ,
3- Department of Exercise Physiology, Faculty of Sport Science and Health, University of Tehran, Tehran, Iran
Abstract: (121 Views)
Background: Aging and consuming a high-fat diet lead to increased oxidative damage to various tissues, oxidative stress is a critical factor in the aging process that can cause direct damage to cellular structure. This study aimed to investigate the Effects of 8 Weeks of HIIT Training on the Nrf2 Gene Expression, lipid peroxidation and Insulin resistance in the pancreas tissue of Aged rats fed a high-fat diet.
Methods: In this experimental study, 20 aged male Wistar rats (age: 18 months and mean weight: 500±100 gr) were randomly divided into four groups including normal food control G1 (n=5), normal food + training G2 (n=5), high-fat food G3 (n=5) and high-fat food +training G4 (n=5). The high intensity interval training program was performed on a treadmill, three days a week for eight weeks. Nrf2 gene expression was performed using real-time PCR and malondialdehyde levels, glucose and insulin were measured using a kit and ELISA method. Data were analyzed by MANOVA test at the P<0.05.
Results: The results of the MANOVA statistical test on the interactive effect of training and diet indicated a significant difference in the insulin resistance index (P = 0.017 and F = 7.17). However, no significant effect was observed for the insulin factor (P = 0.30 and F = 1.13), glucose (P = 0.116 and F = 2.75), MDA (P = 0.87 and F = 0.028), and Nrf2 (P = 0.816 and F = 0.056).
Conclusion: In general, it can be stated that HIIT training in this research can improve insulin resistance by affecting the expression of the Nrf2 transcription factor gene by reducing the oxidant activity in aged rats.
Methods: In this experimental study, 20 aged male Wistar rats (age: 18 months and mean weight: 500±100 gr) were randomly divided into four groups including normal food control G1 (n=5), normal food + training G2 (n=5), high-fat food G3 (n=5) and high-fat food +training G4 (n=5). The high intensity interval training program was performed on a treadmill, three days a week for eight weeks. Nrf2 gene expression was performed using real-time PCR and malondialdehyde levels, glucose and insulin were measured using a kit and ELISA method. Data were analyzed by MANOVA test at the P<0.05.
Results: The results of the MANOVA statistical test on the interactive effect of training and diet indicated a significant difference in the insulin resistance index (P = 0.017 and F = 7.17). However, no significant effect was observed for the insulin factor (P = 0.30 and F = 1.13), glucose (P = 0.116 and F = 2.75), MDA (P = 0.87 and F = 0.028), and Nrf2 (P = 0.816 and F = 0.056).
Conclusion: In general, it can be stated that HIIT training in this research can improve insulin resistance by affecting the expression of the Nrf2 transcription factor gene by reducing the oxidant activity in aged rats.
Type of Study: Research |
Subject:
Special
Received: 2024/04/27 | Accepted: 2024/08/5 | Published: 2024/10/31
Received: 2024/04/27 | Accepted: 2024/08/5 | Published: 2024/10/31
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