Bagher Larijani, Seyed Sajad Mohseni Salehi, Shirin Irani, Marjan Akbari Kamrani, Nasim Sheykh Bahaei, Ahmad Sajadi, Seyed Naser Ostad,
Volume 6, Issue 1 (19 2006)
Abstract
Background: Many researches have been conducted on islet cells' transplantation for a definitive treatment of diabetes mellitus type1. As the viability of the islets is the most important factor in predicting the transplantation prognosis, we have designed a study to isolate rat's islets. The aim of the study was to assess the viability of the islets at different stages and suggest the best transplantation time.
Methods: Pancreatic islets were isolated from male rats (250-300gr) by standard surgical procurement followed by intraductal HBSS distension, chopping and digestion with collagenase (type V). After being centrifuged for 3 times, the islets were then hand-picked and incubated in 37oC with RPMI 1640 media for 6 days. Each well contained 35-45 islets. Viability of islets was assessed by 2 independent investigators, giving score 0-2 to the color of islets under florescent microscope after Propidium iodide/Acridine orange staining at 6 times: just after the incubation, 24h, 48h, 3rd day, 5th and 6th day.
Results: The viability of the islet cells was gradually increased after the incubation as we had the most viability rate after the second day, while it decreased after this period and reached the least rate on the 5th and 6th day.
Conclusion: The islets' viability increased following the cell culture after the isolation procedure, as they have the best condition for transplantation after 48 hours. As the islets’ viability is the most critical point in transplantation, further studies evaluating the effects of different interventions on viability is needed.
Roohangiz Alirezaei Shahraki, Ahmad Aliakbari Kamrani, Robab Sahaf, Yahola Abolfathi Momtaz, Narjes Khosravi Samani,
Volume 17, Issue 5 (7-2018)
Abstract
Background: Diabetes is a chronic disease that is increasing in the world that has a different outbreak in different societies. 90% to 95% of all types of diabetes include type 2 diabetes. This disease is spreading due to the Industrial life and urbanization .The Nationwide Program for Prevention and Control of Diabetes is already under way for controlling the disease and its side-effects. This study is intended to probe into the effects of the program in the country.
Methods: This study used a cross-sectional pre- and post-test design involving 100 elderly patients newly introduced to be suffering from diabetes who were selected by convenience sampling in Isfahan. The data collection methods were a questionnaire on demography and health, and another one on patients’ levels of fasting blood sugar (FBS) and hemoglobin glycosides (HbA1c(The data was gathered before and three months after the patients’ entrance into the program.
Results: The mean and standard deviation of the fasting blood sugar before the program were 172.73 and 71.68 and after the program 143.02 and 51.19, respectively (P<.001; t=5.30). And finally, glycated hemoglobin mean and standard deviation before the program were 8.24 and 1.9 and after the program 7.44 and 1.66, respectively (P<.001; t=4.79).
Conclusion: The results indicate the importance of the program for the prevention and control of the elderly patients’ diabetes by controlling their fasting blood sugar and glycated hemoglobin.It is recommended that this program be implemented in all health centers of the country.
