Showing 14 results for Ayat
Mitra Neiafar, Fatemeh Esfahanian, Alireza Esteghamati, Ramin Heshmat, Mehdi Hedayati, Masoumeh Karami, Mehrshad Abbasi, Manochehr Nakhjavani,
Volume 4, Issue 4 (17 2005)
Abstract
Background: Oxidized low-density lipoprotein (Ox-LDL), a key factor in the development of atherosclerosis, can cause endothelial dysfunction and augment lipid accumulation within the arterial wall. Increased oxidative stress in diabetes contributes to this process. Ox-LDL is a highly immunogenic molecule and it is not clear whether anti oxidized LDL antibodies (OLAB) are pathogenic or protective in atherosclerosis? The aim of this study was to evaluate Ox-LDL and its antibody in type 2 diabetes and healthy subjects.
Methods: As a case-control study we evaluated 81 type 2 diabetic patients and 69 non-diabetic healthy persons aged 40 to 65 years. Controls were sex and BMI matched with diabetic patients. Patients with history of cigarette smoking, antioxidant or antihyperlipidemic drugs consumption, coronary heart disease, hypertension , and renal impairment were excluded. We measured serum level of Ox-LDL(two monoclonal antibody of Mercodia co.) and OLAB by ELISA. Lipid profile, serum electrolytes, and HbA1c (HPLC) were also determined. Ox-LDL and its antibody were compared between diabetic patients and controls and the correlation with lipid profile, HbA1c and BMI were assessed.
Results: Serum Ox-LDL concentration and Ox-LDL to LDL ratio were distinctively higher in controls (15.7+-6.9 vs. 11.8+-5.6, P < 0.005). Ox-LDL concentrations were correlated with LDL-C (rs=0.36, P<0.0005) and total cholesterol (rs=0.31, P<0.0005) in both groups but not with age and HbA1c. In diabetic patients, Ox-LDL and its antibody were positively correlated (rs=0.26, P<0.05). Obese diabetic patients (BMI > 30) had higher Ox-LDL concentrations in comparison with diabetic patients with BMI less than 30.
Conclusion: In diabetic patients Ox-LDL level is lower than non-diabetics and is correlated with its antibodies. Based on previous findings, we suppose that the pattern of LDL oxidation enhances Ox-LDL recognition by macrophage via specific legends. This results in low serum Ox-LDL concentrations in diabetes.
Mahmood Khayatian, Bijan Farzami, Ebrahim Mirzajani, Bagher Larijani, Mohammad Taghikhani, S. Zahra Bathaei, Safoora Vardasbi, Esmael Elmi-Akhouni,
Volume 5, Issue 2 (18 2005)
Abstract
Background: Glucokinase serves as a glucose sensor in pancreatic β-cells and plays a key role in glucose homeostasis and glucose-stimulated insulin secretion (GSIS). In the present study we examined the effect of glucosamine, a glucokinase inhibitor, on the pancreatic glucokinase and hexokinase activities and on insulin secretion from freshly rat pancreatic islets of Langerhans. Insulin concentration was measured by rat insulin ELISA kit.
Methods: The pancreatic islets from normal and type 2 diabetic (nSTZ) rats were isolated by collagenase digestion method. Glucose phosphorylation was quantitated by measuring the rate of glucose-6-phosphate formation in the fluorometric assay. Insulin secretion from hand-picked islets was evaluated in static incubation system. Insulin concentration was measured by rat insulin ELISA kit.
Results: Our findings demonstrate that glucosamine in a dose dependent manner, reduced glucokinase activity in islet extract, but had no effect on hexokinase activity. The glucose-stimulated insulin secretion, was inhibited by glucosamine but it had no effect on the basal insulin secretion. In diabetic rats glucokinase was decreased while the basal insulin secretion and the activity of hexokinase were higher than normals.
Conclusion: Based on results obtained from the present study, the assumption could be made that the decrease in the activity of glucokinase of pancreatic islets could be related to the impaired glucose stimulated insulin secretion. The increase in basal insulin secretion of diabetic rats may be due to an increase in pancreatic hexokinase activity.
Mahmood Khayatian, Bagher Larijani, Bijan Farzami, Shirin Pournourmohammadi, Hoda Boushehri,
Volume 6, Issue 1 (19 2006)
Abstract
Background: Sulfonylurea agents such as Glibenclamide (Glyburide) have been widely prescribe in treatment of type 2 diabetic patients for decades, but controversy remains about their precise mechanism of action. On the other hand, glucokinase serves as a glucose sensor in pancreatic β-cells and plays a key role in the regulation of insulin secretion and glucose homeostasis. The aim of the present study was to evaluate the effect of Glibenclamide on insulin secretion and glucokinase activity in the rat isolated pancreatic islets of Langerhans.
Methods: The islets from normal and type 2 diabetic rats were isolated by collagenase digestion method. Glucokinase activity was measured via determination the rate of glucose-6-phosphate formation in the fluorometric assay. Insulin secretion from hand-picked islets was evaluated by static incubation technique. Insulin concentration was measured by rat insulin ELISA kit.
Results: Our findings obtained from incubation of Glybenclamide with pancreatic islets revealed that this agent increases basal insulin secretion (at 2.8 mM glucose) in both normal and diabetic rats as compared it with control islet (without drug). However, the increase of insulin secretion in response to 16.7 mM glucose was not significant. On the other hand, Glybenclamide had no activating and/or inhibiting effect on pancreatic glucokinase activity in both diabetic and normal Rats. But reduced activity of this enzyme in diabetic rats was significant in comparison with normal.
Conclusion: These data show that increasing effect of Glybenclamide on insulin secretion is through a mechanism other than affecting Glucose Mediated Insulin Release. Moreover, the regulation of pancreatic glucokinase does not depend on glybenclamid.
Bita Faam, Maryam Zarkesh, Maryam Sadat Daneshpour, Fereidoun Azizi, Mehdi Hedayati,
Volume 13, Issue 2 (1-2014)
Abstract
Background: It is now evident that adipose tissue functions as an endocrine organ by releasing
adipokines, and the levels of a number of inflammatory markers elevated in overweight and obese
individuals. The objective of this study was to examine the association between inflammatory markers
(IMs) including C-reactive protein (hs-CRP), Interleukin-6 (IL-6), Homocystein (Hcy) and obesity
variables in Tehran Lipid and Glucose Study (TLGS) adults.
Methods: In this cross-sectional study, 352 individuals (132 men and 220 women), age ≥19 years,
were randomly recruited from among TLGS population. Individuals were categorized based on the
waist circumference. The serum levels of IMs were determined using the Enzyme Linked
Immunosorbent Assay (ELISA) method.
Results: The mean age of participants was 46.1±16.1years and Abdominal obesity were present in
199(56.5%) individuals. The levels of hs-CRP and IL-6 were higher in abdominally obese group
(1507±3.3 vs. 577.8±4.3 ng/mL p<0.001) (3.6±3.3 vs. 1.9±3.8 pg/mL p< 0.001), and in the same
group, the best predictors (based on the adjusted R2) for hs-CRP, IL-6 and Hcy were waist (WC),
waist to height ratio (WHtR) and wrist, respectively. Hip and WHtR were the best predictors for Hcy
and hs-CRP in normal group there was no variable significantly correlated with IL-6, therefore it was
not possible to consider an independent predictor for IL-6.
Conclusion: According to this study, obesity is associated with IM levels, and in abdominally obese
group, the best predictor for Hcy, hs-CRP and IL-6 were Wrist, waist and WHtR respectively.
Toktam Mahmudzadeh, Marziyeh Saghebjoo, Ali Seghatol Eslami, Mehdi Hedayati,
Volume 13, Issue 3 (3-2014)
Abstract
Background: Diabetes is considered as one of the health problems in all societies. Exercise training
and drug interventions, especially herbal medicines, consider as approaches to improving diabetes.
The objective of this study was to investigate the effect of 6 weeks of aerobic training and Pistacia
atlantica extract consumption on pancreatic β-cells function in streptozotocin-induced diabetic rats.
Methods: Thirty six male Wistar rats divided into five groups: normal control, diabetic control,
diabetes+aerobic training, diabetes+extract, diabetes+aerobic training+extract. Diabetes was induced
by intra-peritoneal injection of streptozotocin (40mg/kg). Exercise program was include 6 weeks of
aerobic training on a treadmill (5 sessions per week, 40 minutes per session, with a speed of 20m/min
and 5% incline). Extract groups were received Pistacia atlantica extract based on body weight, at the
end of each training session. After 6 weeks of aerobic training and Pistacia atlantica extract
consumption, plasma glucose and insulin levels were measured for determination of β-cells function
(HOMA-B). Data were analyzed via ANOVA, with a significant level P<0.05.
Results: HOMA-B index in diabetic control group was significantly higher than normal control group
(P=0.0001). Also mean of HOMA-B index in diabetes+extract and diabetes+exercise training+extract
groups was significantly lower than diabetic control group (P values 0.003, 0.001 respectively) and
between mean of HOMA-B index in diabetic control group with diabetes+aerobic training group were
observed no significant differences (P=0.052).
Conclusion: The results of this study showed that Pistacia atlantica extract consumption, alone and
along with aerobic training leads to significant improvement in pancreatic β-cells function in diabetic
rats. Also extract consumption along with aerobic training in comparison to training alone has had
greater impact on the improvement of pancreatic β-cells function. It seems that aerobic training along
with the use of specific herbal plants can be considered as a complementary therapy in improvement
of diabetics.
Zahra Jamshidi Khezerlou, Sajad Ahmadizad, Mehdi Hedayati, Hiwa Rahmani, Azade Movahedi,
Volume 13, Issue 4 (5-2014)
Abstract
Background: The aim of this study was to compare responses of Visfatin and insulin resistance index to various resistance exercise protocols.
Methods: Ten healthy male subjects performed three resistance exercise protocols including maximal strength (three sets of 5 repetition at 85% of 1-RM with 3-min rest between sets), hyperthrophy (three sets of 10 repetition at 70% of 1-RM with 2-min rest between sets) and strength-endurance (three sets of 15 repetition at 55% of 1-RM with 1-min rest between sets) in three separate sessions. Two blood samples were taken before and after resistance exercise protocol. Responses to different resistance exercise protocols were compared by using repeated measures of ANOVA (3×2).
Results: Irrespective of resistance exercise protocol, results showed that plasma visfatin reduced significantly (P<0.05) in response to resistance exercise. Between group comparisons revealed that reductions in visfatin concentration in response to strength-endurance and hypertrophy protocols were significantly higher than maximal strength protocol (P<0.05). Analysis showed that not only glucose, insulin and insulin resistance index did not change in response to resistance exercise significantly, but also there was no significant difference among the responses to different resistance exercise protocols (P>0.05). In addition, there was no significant relationship between changes in visfatin and other parameters (P>0.05).
Conclusion: It could be concluded that performing strength-endurance and hypertrophy protocols that cause reductions in visfatin, possibly due to changes in growth hormone during these protocols, could be beneficial in reducing the hyperinsulinemia.
Abass Bayat Asgari, Sahar Mossanejad, Mina Asgari, Abass Ramazani Farani, Javad Javaheri,
Volume 14, Issue 2 (1-2015)
Abstract
Background: Having high knowledge in patients is not sufficient for disease control and it is necessary to get more skills and capabilities that could be helping in confronting with diabetes associated stresses. This study is aim to assess the relationship between coping styles approaches with adherence to treatment in type 2 diabetic patients. Methods: In a cross sectional design 100 type 2 diabetic patients were selected by convenience route. Folkman and Lazaros as well as adherence to treatment questionnaires were filled by participants. Data was analyzed by SPSS software version 16, spearman correlation test and linear regression model were used for inferential statistics. Results: Problem and excitement oriented subscales were significant correlation. The correlation between problem oriented subscale and adherence was positive and significant (P<0.05) so that regression model showed as this subscale increase the adherence was increasing as well. The correlation between excitement oriented subscale and adherence was negative and significant (P<0.05) so that regression model showed as this subscale increase the adherence was decreasing. Conclusion: The results emphasized that there is significant correlation between copying styles and adherence to treatment in type 2 diabetic patients.
Maryam Sadat Daneshpour, Bahareh Sedaghatikhayat, Mehdi Hedayati, Fereidoun Azizi,
Volume 14, Issue 4 (5-2015)
Abstract
Background: The prevalence of non-communicable disorders such as metabolic syndrome (MetS) is high in developing countries. Metabolic syndrome is a disorder of energy utilization and storage, diagnosed by a co-occurrence of three out of five of the following medical conditions: abdominal (central) obesity, elevated blood pressure, elevated fasting plasma glucose, high serum triglycerides, and low high-density cholesterol (HDL) levels. The present review aims to discover the genetic variant reported in association with MetS. Methods: The database for genotypes and phenotypes (dbGaP) and the database for genetic associations and human genome (HuGE navigator) were utilized in order to search for genes and their corresponding polymorphisms related to MetS. Additionally, an electronic literature search for other Iranian studies and the genetic aspect of TLGS was completed using PubMed. Results: For phenotype selection in PheGenI, 30 traits were chosen and after the analysis, 21 of them were in common results with MetS. After finding the common variation between traits and MetS, omitting the repeated SNPs, 173 variations were remained. Finally, results distinguished six of the most important genetic regions found to have strong association with MetS. Conclusion: Identifying major genes that are responsible for the metabolic syndrome may improve the medical care for treating individuals with metabolic syndrome, and eventually may lead to personalized medicine in which treatment is tailored genetically to the patient’s needs. The present candidate regions is a respectable start to replicate genetic studies in large affected Iranian individual which we hope leads us to improve our medical care in this field.
Bita Faam, Asieh Zahedi, Mehdi Hedayati, Fereidoun Azizi, Mohammad Ali Mansournia, Maryam Daneshpour,
Volume 15, Issue 1 (1-2016)
Abstract
Background: The scavenger receptor class B type I (SR-BI), as the high density lipoprotein cholesterol (HDL-C) receptor, is a key component in the reverse cholesterol transportation. The objective of this study was to assess the association between exon1 (G→A) polymorphism of SR-BI gene and lipid profiles among the Tehran Lipid and Glucose Study (TLGS) population.
Methods: This cross-sectional study included 774 adults (322 males and 452 females) aged 20–70 years, who were randomly selected from among TLGS population. Anthropometrical and biochemical variables for participants were measured. Selected SR-BI gene polymorphism was determined with restriction fragment length polymorphism (RFLP) using the Alu restriction enzyme.
Results: according to the results of current study, in the Tehran population, the allele frequency of SR-BI (G→A) polymorphism was 0.159 for an allele (minor allele) and 0.841 for G allele. Allele frequencies were in conformity with Hardy–Weinberg equilibrium. The result of this study showed that Subjects with the less common allele (allele A), after adjusting for age, have lower HDL-CandHDL3concentrations (p=0.046, p=0.041 respectively).
Conclusion: lipid disorders are caused by the interaction of environmental and genetic factors; therefore, exon1 (G→A) polymorphism of SR-BI gene could not be the only cause for the abnormality in the HDL-C levels. In future, this polymorphism may be use as a molecular marker for diagnosis.
Zahra Sadeghian Far, Abdolkarim Sheikhi, Seyed Mohammad Gheibi Hayat,
Volume 16, Issue 6 (10-2017)
Abstract
Background: Usually excess feeding in stored in the body as fat and leads to overweight. One of the causes of catching type 2 diabetes is obesity. Proinflammatory cytokines such as TNF- α and IFNγ can damageTnf- Α And Ifnγ By Peripheral Blood Mononuclear Cells Of Patients With Diabetes Mellitus Type Ii Admitted To Ganjavian Dezful Hospit insulin signaling in insulin-sensitive tissues. In this study, the effect of garlic extract on the expression of TNF-α and IFNγ secreted by the mononuclear cells was measured.
Methods: After taking peripheral blood from patient with type 2 diabetes, mononuclear cells were extracted by Ficoll Method. Cells were a culture with two different concentration of garlic extract (10% and 30%) for 48 and 24 hours. Then for measuring TNF-α and IFNγ release level, ELISA method was used.
Results: Garlic extract can reduce the amount of TNF-α secretion from cells mononuclear, although the timing and concentration of the extract had no effect on the results. Moreover, garlic extract had no effect on the secretion of IFNγ.
Conclusion: Using garlic extract can be adopted therapeutic strategies aimed at adipose tissue inflammation and insulin resistance that is useful for the prevention and treatment of type 2 diabetes.
Laleh Ranjbar, Farzaneh Taghian, Mehdi Hedayati,
Volume 17, Issue 4 (5-2018)
Abstract
Background: Apelin is an Adipokine which is recently discovered and widely secreted from white adipose tissue and in fat and overweight person, apelin values and gene expression increase .In this study, the effect of one period aerobic exercise and vitamin D consumption on weight, plasma apelin values and insulin resistance in overweight women was researched.
Method: In order to doing this reasearch 40 women whom have over weight with , average old , weight , height, BMI and WHR respectively : (30.37 ± 6.91) years old , (74.89 ± 12.97) kg , (157 ± 7.02) m , (30.08 ± 3.95) kg/m2 , (0.81 ± 0.07). After passing the medical sepration dived to 4 experimental group: 1st experiment group (aerobic exercise) n=10, 2nd experimental group (aerobic exercise and vitamin D) n=10, 3rd experimental group (vitamin D) n=10 and control group n=10. At first body composition was measured th included: BMI, WHR, weight, waist and hip size. After that the blood sample was taken from triables. (Fasting) and amount of the apelin, glucose, insulin, vitamin D, triglycerides, cholesterol, LDL and HDL were measured. Then experimental group 1 and 2 were encounterd and emotionalized by an aerobic exercise (treadmill) after 10 weeks all of the measure mented conversionxls were measure again befor the test. For comparisoning of the groupa that befor and after the test was used from –t test and if was used from the variance test for comparisoning two group.
Results: results showed that after 10 week of aerobic exercise an vitamin D using : BMI, WHR, apelin, cholesterol, LDL, glucose, insulin and insulin resistance were reduced, a triglyceride also decrease and HDL had a significant increasing.
Conclusion: Result confirmed the positive effect of the aerobic exercise on body compositions apelin and plasmas Lipoprotein and also showed that using vitamin D using in obese people and people who have overweight can be beneficials.
Asieh Khosravanian, Saeed Ayat,
Volume 18, Issue 2 (2-2019)
Abstract
Backgrounds: Early detection of diabetes is critical to avoid complications and damage caused by this disease. The purpose of this paper is designing an intelligent system for Diabetes prediction (healthy or patient) by using regression method based on Multilayer Perceptron Neural Network.
Methods: In this descriptive-analytic study, an intelligent system is designed to classification diabetes patients. The system is simulated by MATLAB software 2015 (8.5.0.197613). In this study, used PID dataset in UCI Machine Learning Repository. The dataset is contained 768 records from Indian women and 8 diagnostic factors for Diabetes.
Results: The data were then divided randomly in 20 groups for training and testing, after preprocessing. 90% of the data is used for training phase and 10% for the test phase. The results obtained based on sensitivity, specificity, accuracy and precision were 0.4815, 0.9804, 0.8077 and 0.9286, respectively.
Conclusion: The obtained results, showed superiority of designed intelligent system to classify individuals (healthy and patient) in comparison with other methods implemented on this dataset. Using MLP- Regression has increased the accuracy of the proposed system.
Parisa Dana, Nasim Hayati Rodbari, Parichehreh Yaghmaei, Zahra Haj Ebrahimi,
Volume 21, Issue 4 (10-2021)
Abstract
Background: Empagliflozin, selective glucose-sodium inhibitor of the latest drugs in the treatment of type 2 diabetes. Diabetes induced hypogonadism disrupts sexual function. There is a direct relationship between reducing blood glucose and reduced libido. In this project, the anti-diabetic drug Empagliflozin in addition to treatment has been studied, in terms of effect on sexual function.
Methods: Type 2 diabetes with injection of 60 mg/kg streptozotocin per body weight was create intraperitoneally. The primary diabetes was treated with two doses of 10 and 25 mg/kg Empagliflozin per body weight. Sexual hormones, biochemical parameters and lipid profile were measured. Evaluation of Sperm parameters and morphological studies were performed on testicular tissue, pancreas and epididymis. Data were analyzed by one-way ANOVA in SPSS software version 22 and P˂0.05 was considered as a significant level.
Results: Empagliflozin significantly increases the quality, survival and natural state of sperm head and tail. Empagliflozin reduces weight in diabetic rats. Empagliflozin Without increasing hyperglycemia by reducing blood glucose affects steroidogenesis, maintains fertility strength in testicles, and increases the level of sex hormones. Empagliflozin significantly increases insulin secretion. Empagliflozin maintains and integrity of pancreatic beta cells. Empagliflozin has no effect on lipid profile.
Conclusion: Empagliflozin in addition positive effects on the treatment of diabetes in the early stage improves sexual function in adult rats.
Marayam Arabloei Sani, Parichehreh Yaghmaei, Zahra Hajebrahimi, Nasim Hayati Roodbari,
Volume 22, Issue 3 (9-2022)
Abstract
Diabetes is a serious public health problem in low- and middle-income countries. There is a strong association between hyperglycemia, oxidative stress and the development of diabetes. Therefore, screening and early diagnosis of this disease in people at high risk can be effective in preventing these complications. The main route of insulin signaling is to control lipid and glucose metabolism, and its imbalance leads to type 2 diabetes. P-Cymene is an aromatic monoterpene with a wide range of therapeutic properties including antioxidant activity. The aim of this study was to evaluate the effect of p-Cymene at a dose of 50 mg / kg in preventing the symptoms of diabetes and serum factors.
Materials and Methods: 30 male Wistar rats weighing approximately 250 g were randomly selected and divided into five groups: control, diabetic control (sham), metformin-treated diabetic (met), and non-diabetic treated. P-Cymene and diabetics treated with P-Cymene were divided. One week after streptozotocin injection, treatment with P-Cymene at a dose of 50 mg / kg was performed by sunflower oil solvent for 28 days by gavage. At the end of the experiment, blood samples were taken from the hearts of rats and the parameters of glucose, insulin, and superoxide dismutase (SOD) were measured.
Results: The results showed that diabetes increased water and food intake as well as glucose levels and decreased insulin (P <0.001) and superoxide dismutase activity in rats and administration of P-Cymene extract improved the symptoms of diabetes and oxidative stress in Became diabetic rat.