Showing 2 results for Barari
Hadi Afshar Bakeshlou, Ahmad Abdi, Mohammad Ali Azarbayjani, Alireza Barari,
Volume 19, Issue 2 (1-2020)
Abstract
Background: Diabetes is a chronic and progressive metabolic disorder that leads to more severe cardiac complications. The aim of the present study was to investigate the effects of Berberine chloride at a dose of 50 mg / kg on the heart tissue of streptozotocin-induced diabetic rats with aerobic training.
Methods: 56 male Wistar rats were randomly divided into seven groups (n = 8): control (C), sham (S), Aerobic training control (TC), diabetes mellitus (DM), diabetes-berberine (BDM), diabetes mellitus. Aerobic training (TDM), and aerobic training-berberine (TBDM) were divided. Diabetes was induced by injection of streptozotocin in male rats. The training groups performed aerobic exercise program (10-18 m / min, 10-40 min five days a week) for six weeks on the treadmill for histological evaluation using hematoxylin and eosin (H&E), Masson trichrome and staining Immunohistochemicals were used to measure diameter change, cardiomyocyte rupture, change in nuclei, and collagen deposition in cardiac muscle fibers using one-way ANOVA and Tukey post hoc tests with SPSS 21 software.
Results: The results showed that DM group did not induce cardiomyocyte fibers rupture and collagen deposition and reduction of filament diameter in group C, S and TC and its damage in heart tissue was less in TBDM group than in BDM and TDM groups.
Conclusion: The results showed that berberine supplementation reduced these effects and synergized with aerobic training and reduced the cardiac cardiomyocyte muscle fibers diameter and decreased collagen deposition and better order of nuclei.
Alireza Zandinezhad, Hossein Abednatanzi, Farshad Ghazalian, Alireza Barari,
Volume 23, Issue 3 (9-2023)
Abstract
Background: The aim of the present study was to study the changes in the expression of Cox-1 and Cox-2 genes in liver tissue and tumor volume after aerobic exercise and consumption of pineapple extract in mice with skin cancer.
Methods: This fundamental-laboratory study was conducted on 32 male C57BL/6 mice in four groups including control, aerobic exercise, pineapple extract and aerobic-pineapple exercise. After tumor induction, the animals underwent aerobic training program for six weeks and pineapple extract was gavage at 300mg/kg. The weight and tumor volume of mice were measured. After obtaining blood and tissue samples, expression of Cox-1 and Cox-2 genes in liver tissue was done by RT-PCR method. Then the data were analyzed using one-way analysis of variance, two-factor analysis of variance and post hoc test, and the significance level of p≤0.05 was considered.
Results: Compared to the control group, aerobic exercise and pineapple-exercise showed a significant decrease in tumor volume and a decrease in the expression of Cox-1 (0.59±0.97) and Cox-2 (0.5±0.4) gene expression in the aerobic-Pineapple compared to the control group (1±0) (p≤0.05). Cox1 gene expression increased significantly in the Aerobic exercise group, but a significant decrease was observed in the pineapple-Aerobic group. Also, Cox2 gene expression in the liver tissue in the exercise group Aerobic and interactive Aerobic-pineapple group had a significant decrease compared to the control, but in the pineapple group compared to the control, the difference in the expression of this gene was not significant.
Conclusion: The results of the present study showed that the expression of Cox 1 gene was significantly increased in the aerobic training group and significantly decreased in the pineapple-training group. Cox-2 gene expression in the liver tissue in the aerobic training group and the pineapple-training interactive group had a significant decrease compared to the control, because the Cox-2 enzyme is related to inflammation and pain, angiogenesis, cancer and Alzheimer's disease. Therefore, it can be concluded that its inhibition or reduction can be considered as a promising and effective strategy for the treatment and prevention of cancer.
