Parvin Farzanegi, Farzaneh Shokrian,
Volume 15, Issue 1 (1-2016)
Abstract
Background: Type 2 diabetes is a chronic disease that leads to many complications such as atherosclerosis. Matrix Metalloproteinase (MMPs) family plays a key role in atherosclerosis and remodeling of the vessel wall. The aim of this study was determination the Effect of eight weeks aerobic exercise with Portulaca oleracea seeds consumption on MMP-1, MMP-3 and MIP-1α in women with type 2 diabetes.
Methods: In one Quasi-experimental study 28 women with type II diabetes (44 to 65 years old) selected and divided into 4 groups (Exercise, Supplement, Exercise-Supplement and Control groups) randomly. Exercise program included 8 weeks (3 sessions per week) with 50 to 70% of maximum heart rate. Supplemented groups received 7.5 g Portulaca oleracea seeds every day. Blood samples were collected before and after the 8 weeks with 12 hours fasting conditions. P < 0.05 considered statistically significant.
Results: Eight weeks of the aerobic training or portulacalo consumption cause a significant decrease in MMP-1، MMP-3 و MIP-1α levels, in comparison to pretest. However, the effectiveness of combination training and supplement is better in the above indicators. MMP-1 levels showed a significant difference between supplement, training, training- supplement group with control group (P = 0.001) and between supplement exercise group with training- supplement (Respectively P = 0.018, P =0.039). MMP-3 levels showed a significant difference between supplement with training- supplement (P = 0.007) and control group (P = 0.011), exercise group with training- supplement (P = 0.026) and control group (P = 0.04). MIP-1α levels showed a significant difference between training- supplement and control group (P = 0.001).
Conclusion: The results showed both intervention exercise and purslane may be support from due to the effects of antioxidant and anti-inflammatory from oxidative stress induced diabetes and combination therapy was associated with synergistic effects.
Majid Jahani, Hasan Matinhomaie, Parvin Farzanegi,
Volume 19, Issue 4 (4-2020)
Abstract
Background: Physical activity plays a major role in the prevention of cardiovascular disease and diabetes, but the effect of intense activity on endoplasmic reticulum proteins and apoptosis and necroptosis in diabetic conditions is unclear. The aim of the present study was to investigate the changes of PERK and CHOP proteins in endoplasmic reticulum of cardiac myocytes of diabetic Wistar rats following continuous and interval exercise.
Methods: For this purpose, 32 male white wistar were purchased and were randomly divided into 4 groups of hemogenus 8 rats in each group: Healthy control (C), Diabetic control (D), Diabetic with moderate-intensity continuous training intensity at the 55min on 26 m/min speed (D+MICT) and Diabetic with high-intensity interval training intensity at the 85-90% of maximum speed (D+HIIT); 5 days/week for 8 weeks. For evaluate changes in the expression of the proteins associated with apoptosis and necroptotic death in the diabetic heart muscle myocardium, based on Western blot analysis will be used. Also, the one-way analysis of variance (ANOVA) is used to determine differences between the study groups.
Results: The results showed that induction of type 2 diabetes increased apoptotic and necroptosis cell death (P≥0.05). Therefore, both continuous and intermittent aerobic exercise modulate apoptotic cell death. And both intermittent and continuous exercise had a significant effect on cell necroptosis death.
Conclusion: It seems that different levels of aerobic exercise have different effects on cardiac myocytes cell death in diabetic rats. But more research is needed to confirm the death of diabetic necroptics.
Afshin Khaman Hatami, Kamal Azizbeigi, Zaher Etemad, Parvin Farzanegi,
Volume 25, Issue 5 (12-2025)
Abstract
Background: Aging is associated with Sarcopenia as well as oxidative stress (OS) caused by Ferroptosis. The aim of the present study was to investigate the effect of aerobic exercise training (AT) on OS caused by ferroptosis in sarcopenia model mice.
Methods: Twenty-one C57BL/6 mice (16-24 wks. age) sarcopenia model with an average weight (25-35 g), and 21 healthy mice (6-7 weeks age) were randomly assigned to the following (1) healthy-young control (HYC; n= 7), (2) aerobic training-young (ATY; n= 7), (3) healthy-old control (HOC; n= 7), (4) old control-sarcopenia model (OCS; n= 7), (5) aerobic training-healthy-old (ATHO; n= 7), (6) healthy-old sarcopenia model (HOS= 7). The intervention group underwent AT for eight weeks, five sessions per week at an intensity of 60-80% of aerobic capacity (VO2max). The expression of the glutathione peroxidase (GPX-4) and superoxide dismutase (SOD) genes was measured using Real Time PCR. The Malondialdehyde (MDA) and Fe²⁺concentration in the gastrocnemius muscle was also measured.
Results: It was observed that the enzymes gene expression of SOD and GPX-4 in the (OSC) was significantly decreased compared to the (HOC) (P= 0.001, P= 0.002, respectively), and the MDA and Fe²⁺ was significantly increased (P= 0.001; P= 0.002, respectively). Also, the of SOD and GPX-4 gene expression of enzymes in the ATY, ATHO was significantly increased compared to the HYC, and HOS (P= 0.001; P= 0.002, respectively), and the concentration of MDA and Fe²⁺ was significantly decreased (P= 0.001; P= 0.002, respectively).
Conclusion: Finally, it can be said that aerobic training reduces oxidative stress caused by ferroptosis in Sarcopenia by increasing the enzymes GPX and SOD gene expression and reducing the concentration of MDA and Fe²⁺.
