Showing 3 results for Feizolahi
Elham Mokhtari, Amir Sarshin, Foad Feizolahi, Eidi Alijani,
Volume 22, Issue 2 (7-2022)
Abstract
Background: Type 1 diabetes is associated with decreased skeletal muscle capillary and improper regulation of angiogenesis pathways in skeletal muscle. This research intended to study the effect of resistance training and endothelial stem cell injection on βeta-actin, phosphorylated and total AKT of skeletal muscle in type 1 diabetic rats.
Methods: In this experimental study, 36 male Wistar rats (age 6 weeks) were divided into six groups of control (healthy), basal diabetic control, diabetic control, diabetes + stem cell injection, diabetes + resistance training and diabetes + stem cell injection + resistance training. In this study, rats became diabetic intraperitoneally using streptozotocin as a single dose of 40 mg/kg. Resistance exercises including climbing a one-meter ladder with weights hanging from the tail were performed for 17 sessions. 500,000 bone-derived stem cells were injected by a cell counter. The levels of βeta-actin, phosphorylated and total AKT in skeletal muscle tissue of rat were measured by using the Western blotting method.
Results: The results showed that resistance training led to significant increase in Pho-AKT, β-actin and Pho-AKT/AKT ratio and significant decrease in AKT of muscle tissue in type 1 diabetic rats (P<0.001). Injection of stem cells leads to significant increase in Pho-AKT and Pho-AKT/AKT ratio and resistance training with simultaneous injection of stem cells leads to significant increase in Pho-AKT, β-actin and significant decrease in Akt of muscle tissue in type 1 diabetic rats (P<0.001).
Conclusion: According to the results, it is possible that the intervention of resistance training with injection of stem cells can help regulate the pathways of skeletal muscle angiogenesis in type 1 diabetes.
Morteza Chenari, Alireza Rahimi, Amir Sarshin, Foad Feizolahi,
Volume 22, Issue 2 (7-2022)
Abstract
Background: Damage to the heart tissue in diabetics causes inflammation and destruction of heart cells, which in turn leads to apoptosis or cell death. The aim of this study was to investigate compare the effect of six weeks of aerobic and resistance training on apoptotic indice of caspase-8 and catalase in the heart tissue of male diabetic rats.
Method: In this experimental study, 24 male Wistar rats were divided into six groups: aerobic training, resistance training, aerobic sham, resistance sham, control and healthy. Diabetes was induced by intraperitoneal injection of a single dose of streptozotocin in the amount of 30 mg per kg. The aerobic and resistance training program was performed for six weeks. Western blotting was used to measure caspase-8 and catalase. Data were analyzed by one-way analysis of variance and Tukey post hoc test at the P<0.05.
Results: The results showed that the mean difference of caspase-8 between aerobic training group and healthy group (P=0.752), resistance training group with healthy group (P=0.723) and resistance training with aerobic training group (P=1.00) were significant. Caspase 8 was lower in the aerobic exercise group than in the resistance exercise group. The difference between the mean catalase between the aerobic training group with the healthy group (P=0.024) and the aerobic training group with the resistance training group (P=0.023) was significant and the amount of catalase in the resistance training group was higher than aerobic training.
Conclusion: Aerobic and resistance training can reduce the apoptotic index of caspase-8 and increase catalase in the heart tissue of diabetic rats.
Masomeh Sarmadiyan, Eidy Aliijani, Fuad Feizolahi, Davood Khorshidi,
Volume 22, Issue 3 (9-2022)
Abstract
Background: Type 1 diabetes is a disorder caused by autoimmune destruction of pancreatic insulin-producing cells. This induction of autoimmunity may be due to genetic and environmental factors. Bax and Bcl2 proteins play an important role in the process of apoptosis.
Methods: In this study, 30 male Wistar rats weighting approximately 200±20gr were randomly selected from available rats in lab (500). Subjects after 2 weeks of familiarity with the environment were randomly divided into 5 groups of 6, including (diabetes + injection, exercise) and (diabetes + exercise) and (diabetes + injection) and (diabetes control to control the passage of time) and (basic diabetes to Defaults) under the same laboratory conditions and developed type 1 diabetes by intraperitoneal injection of streptozotocin (stz) (60 mg / kg). Rats in the diabetic group and the diabetic group + stem cell injection had a total of 17 sessions of resistance training for 5 weeks.
Results: The results of the present study showed that there was no significant difference between the mean of Bax and Bcl2 in the resistance training group with simultaneous injection of stem cells and the training group.
Conclusion: The results of our study showed that performing 17 sessions of resistance training alone with stem cell injection was effective on the expression of Bax protein and Bcl2, but there was no significant difference between the effect of training alone and training with stem cells.
