Showing 3 results for Gharib
Homeira Zardouz, Saleh Zahedi Asl, Mohammad Kazem Gharib Naseri,
Volume 5, Issue 4 (17 2006)
Abstract
Background: Regarding the adverse effect of stress on glycemic control in type 2 diabetic patients, the present study investigates the function of Glibenclamide on insulin release from β cells of rat pancreatic islets, subsequent to chronic psychological stress exposure.
Methods: In this study 30 male Wistar rats were divided into equal groups of control and experiment (5 groups). Four different restraint stressors with random sequence were used 1h twice daily for 15 and 30 days. 24 hours after the last stress session, static insulin secretion from isolated pancreatic islets of each animal were evaluated in the presence of 5.6, 8.3 and 16.7 mM glucose. Also insulin release in response to 5.6 mM glucose in the presence of 10 μM Glibenclamide was evaluated.
Results: The insulin release from isolated islets of the stress experienced animals was significantly increased only on the 30th day as compared to the control animals. In the experiment group, insulin release from the islets in the presence of 5.6 mM glucose alone was significantly increased on the 15th and 30th days as compared to the first day. However, in the control group there was no significant increase in insulin release at the similar conditions. In contrast to the control group, insulin release in response to 5.6 mM glucose in the presence of 10 μM Glibenclamide revealed no significant difference in the experiment group on the 1st 15th and 30th days as compared to the insulin release in the presence of 5.6 mM glucose alone. Insulin release from the isolated islets exposed to 5.6 mM glucose in the presence of 10 μM Glibenclamide, on different experimental days was not significantly different between the control and experiment groups.
Conclusion: According to the results of this study, it appears that chronic psychological stress decreases the responsiveness of pancreatic β cells to Glibenclamide, subsequently could prevent the augmentation of insulin release induced by the drug. This finding is worthy to consider in metabolic control of diabetic patients whom consume the agent.
Mahnaz Sanjari, Noushin Fahimfar, Fatemeh Hajivalizadeh, Safora Gharibzadeh, Kazem Khalaji, Zohreh Hosseinzadeh, Firozeh Hajipour, Kobra Gorgani, Afshin Ostovar,
Volume 20, Issue 1 (25th Anniversary of the Foundation, Special Issue 2021)
Abstract
Background: Osteoporosis is one of the most important health problems in the country due to fracture. Osteoporosis Research Center with more than 20 years of experience in this field tries to present achievements and activities of the management and treatment of osteoporosis in the country while introducing this center. This review aims to overview the osteoporosis research center activities on osteoporosis.
Methods: In these conventional review national and international databases were investigated on osteoporosis without any restriction on time and language. Also, other activities that are not reflected in the papers were obtained from the professional website and official reports.
Results: According to the strategic plan of the osteoporosis research center, the achievements of this center provided in in three areas of research (population-based studies, clinical studies, basic science studies and health system studies), technology (diagnostic, therapeutic technologies, service delivery models) and education (training students at different levels of education). Supplementary, patient education, general education, and service provider training).
Conclusion: Osteoporosis Research Center is recognized as the only specialized research center in the field of osteoporosis in the country. The center is trying to improve its position as a regional center in the field of osteoporosis by relying on its strategic and operational plan, in addition to maintaining its current position.
Raziyeh Gharib, Ali Khatibi,
Volume 21, Issue 5 (1-2022)
Abstract
Background: Glycation is the non-enzymatic reaction between the carbonyl groups in sugar and free amino groups in proteins. this reaction leads to changes in structure and functions of proteins. Advanced glycation end products (AGEs) is the final stage in this process, which is highly oxidizing and destructive nature, causing many diabetic complications.
Methods: In the present investigation, the effect of fasting upon the glycation process of human Carbonic anhydrase II under physiological conditions (37 °C and pH 7.4) was studied recruiting various techniques including Ultraviolet–visible spectroscopy, fluorescence spectroscopy and CD Spectroscopy. To address this question, different samples of control carbonic anhydrase (without glucose and 3-beta-hydroxybutyrate), carbonic anhydrase with glucose, carbonic anhydrase in the presence of only 3-beta-hydroxybutyrate (BHB) and carbonic anhydrase along with glucose and 3-beta-hydroxybutyrate were incubated for 35 days under physiological conditions.
Results: The results indicate that 3-beta-hydroxybutyrate, which is greatly increased in the body during fasting, functions as an inhibitor of the glycation process and decreases the impacts of glucose binding to the protein and prevents the formation of AGEs and preserve enzyme activity.
Conclusion: Fasting can play an important role in maintaining the health of the body and eliminating the complications of the disease, with a significant increase in the production of 3-beta-hydroxybutyrate as an inhibitor of the glycation process.
