Showing 3 results for Golmohammadi
Manouchehr Nakhjavani, Bijan Farzami, Taghi Golmohammadi, Akbar Jafarnejad,
Volume 2, Issue 1 (17 2003)
Abstract
Background: The non-enzymatic glycosylation (NEG) of proteins in diabetes damages both the structure and function of these proteins. In vivo and in vitro studies have shown that NEG of proteins and advanced glycosylation end-products (AGE) contribute to the pathogenesis of both macrovascular, such as atherosclerosis, and microvascular complications, such as retinopathy and nephropathy, in diabetes.
Methods: We studied the electrophoretic mobility, fluorescence at isoelectric pH, and time-dependent AGE formation of glycosylated albumin. For the first time, we have used isoelectric focusing to study serum glycosylated albumin in diabetic patients and healthy controls. Results: After 10 weeks incubation with glucose, the electrophoretic mobility of glycosylated albumin increased 21.3% compared with normal albumin. The isoelectric pH of albumin decreased from 4.6 on day 1 to 4.1 on day 7. The increase in electrophoretic mobility was accompanied by the drop in pH during the first week of incubation. These changes correlated well with those observed by fluorescence. The glucose content of the albumin samples decreased during the first week of incubation, but gradually increased thereafter. Fluorescence readings agreed with these observations. Using isoelectric focusing, there was a significant difference between the serum albumin of diabetic and normal individuals (p<0.001).
Conclusion: Increased electrophoretic mobility during the first week with a simultaneous decline in isoelectric pH shows that AGE formation begins after the first week. The reduction in glucose concentration during the first week and its subsequent increase during the second week may be attributed to the formation and hydrolysis of AGE. This method may be used to determine the stability or progress of diabetes.
Hassan Mirzaei, Taghi Golmohammadi, Seyed Mohammad Akrami, Mahmood Doosti, Manochehr Nakhjavani, Ramin Heshmat, Parvin Amiri,
Volume 6, Issue 1 (19 2006)
Abstract
Background: Peroxisome Proliferators- Activated Receptor-Gamma2 (PPAR- γ2) is a nuclear receptor that regulates adipocyte differentiation, lipid metabolism and insulin sensitivity. The aim of this study was to evaluate the association of the Pro12Ala polymorphism at the PPAR- γ2 gene in Iranian population with obesity.
Methods: The genomic DNAs of the 156 subjects including obese and healthy isolated from EDTA whole blood. Pro12Ala polymorphism detected by Polymerase Chain Reaction – Restriction Fragment Length polymorphism (PCR-RFLP).
Results: In the obese group , one sample (1.3%) was as homozygote Ala/Ala genotype , 24 samples (30.8%) were Pro/Ala heterozygote and 53 samples (67.9%)as Pro/Pro genotype were identified . in the control group , one sample (1.3%) was as Ala/Ala genotype , 12 samples (15.4%) were Pro/Ala genotype and 65 samples (83.3%) were Pro/Pro genotype. allele frequencies of Ala in obese subjects (qAla=%16.7)were significantly different from those in control subjects (qAla=%8.9).
Conclusion: Our results revealed that Pro12Ala polymorphism in PPAR- γ2 gene associated with obesity in the Iranian population and presence Ala allele cause to significantly higher BMI and lower fasting blood sugar.
Yeganeh Golmohammadi Samani, Parvaneh Nazarali, Rostam Alizadeh, Najmeh Rezaeinezhad,
Volume 24, Issue 4 (10-2024)
Abstract
Background: Aging and consuming a high-fat diet lead to increased oxidative damage to various tissues, oxidative stress is a critical factor in the aging process that can cause direct damage to cellular structure. This study aimed to investigate the Effects of 8 Weeks of HIIT Training on the Nrf2 Gene Expression, lipid peroxidation and Insulin resistance in the pancreas tissue of Aged rats fed a high-fat diet.
Methods: In this experimental study, 20 aged male Wistar rats (age: 18 months and mean weight: 500±100 gr) were randomly divided into four groups including normal food control G1 (n=5), normal food + training G2 (n=5), high-fat food G3 (n=5) and high-fat food +training G4 (n=5). The high intensity interval training program was performed on a treadmill, three days a week for eight weeks. Nrf2 gene expression was performed using real-time PCR and malondialdehyde levels, glucose and insulin were measured using a kit and ELISA method. Data were analyzed by MANOVA test at the P<0.05.
Results: The results of the MANOVA statistical test on the interactive effect of training and diet indicated a significant difference in the insulin resistance index (P = 0.017 and F = 7.17). However, no significant effect was observed for the insulin factor (P = 0.30 and F = 1.13), glucose (P = 0.116 and F = 2.75), MDA (P = 0.87 and F = 0.028), and Nrf2 (P = 0.816 and F = 0.056).
Conclusion: In general, it can be stated that HIIT training in this research can improve insulin resistance by affecting the expression of the Nrf2 transcription factor gene by reducing the oxidant activity in aged rats.
