Showing 7 results for Habibi
Maryam Chinisaz, Azadeh Ebrahim-Habibi, Parichehreh Yaghmaei, Kazem Parivar, Ahmad- Reza Dehpour,
Volume 13, Issue 4 (5-2014)
Abstract
Background: The flexible structure of proteins is one important factor in the formation of ordered aggregates (amyloid fibril). This is a major problem for therapeutic proteins such as insulin. Study on the induction and inhibition of insulin fibrillation process with specific compounds such as aromatic derivatives may provide useful information about means of stabilization of protein structures.
Methods: To induce fibrillation, regular insulin was incubated in phosphate buffer (pH=7.4) during 24 hours. Amyloid formation was investigated by using Congo red absorbance and transmission electron microscopy (TEM). Then nodular amyloidosis was observed in mice upon amyloid fibril injection, after which the excised nodule was studied by Congo red staining and polarized light microscopy. Then, some aromatic compounds effect was investigated on the fibrillation process.
Results: Regular insulin form mature amyloid fibrils at pH=7.4, 37°C after 24 hours. Silibinin had the highest inhibitory effect on that process. Furthermore, Amyloid fibril injection in mice caused nodular amyloidosis.
Conclusion: Regular insulin has a high potential to undergo amyloid aggregation. Nodular amyloidosis confirms fibril formation by insulin under in vitro condition. Silibinin could be considered as a potential compound capable to increase protein structure stability.
Parichehreh Yaghmaei, Faazaneh Abbasi, Azadeh Ebrahim-Habibi, Atieh Hesaraki,
Volume 13, Issue 5 (7-2014)
Abstract
Background : PCOS (Polycystic Ovary Syndrome = PCOS) is a relatively common disease in women of childbearing age. Bisphenol s are chemical groups that are composed of two functional hydroxyl group and most of them are based on methane. In this study, the effects of phenolic compounds, (bisphenol A and AP) in polycystic ovary syndrome were investigated.
Methods : mature Wister rats were classified in six groups . Healthy controls ( healthy rats that received Grape seed Oil as solvent ), PCOS Group ( disease induced by testosterone propionate ) experimental groups 1.2.3.4 , respectively. after induction of PCOS,they received bisphenol- A and AP in doses of25 mg / kg, 50mg/kg, by gavage . Blood samples were taken and the hormones LH, FSH was measured . Ovaries were also to be studied histologically .
Results : The results showed that the amount of LH to FSH ratio increases after induction with bisphenol-A and bisphenols AP,. Furthermore the number of follicles and growing follicles were reduced significantly, and these concequences causing negative effects on polycystic ovary syndrome.
Hamed Rezaei Nasab, Roohollah Ranjbar, Abdol Hamid Habibi, Saeed Shakerian,
Volume 14, Issue 2 (1-2015)
Abstract
Background: The purpose of this study was to compare three intensities of acute aerobic exercise on plasma visfatin concentration in type 2 diabetic males. Methods: Ten men with type 2 diabetes (mean ± SD age 52.6 ± 3.6 years, height 171.3 ± 6.7 cm, Weight 87.58 ± 4.7 kg) participated in the study. In the first session, anthropometric measurements, body composition, and peak oxygen uptake (VO2peak) was measured in all subjects. In the next sessions, subjects completed three acute aerobic exercises on separate days in a crossover design. The three exercise trials performed at intensity of 40%, 60%, and 80% VO2peak after fasting for at least 10 hours. Energy expenditure was 300 kcal for each exercise trial. Blood samples before exercise, immediately after and 24 hours after exercise were collected for measuring visfatin, glucose and insulin. Results: The results showed no significance interaction (p>0.05) between exercise and sampling time. This analysis also revealed significance in the main effects (p>0.05) of the two factors (exercise and sampling time). Pearson correlation showed that there was significant relationship between visfatin and insulin (p<0.05). Conclusion: According to the present results, we can conclude that acute aerobic exercise at different intensities with 300 kcal energy expenditure will not change visfatin levels in type 2 diabetes.
Mahsa Soltani-Nobakht , Parichehreh Yaghmaei, Azadeh Ebrahim-Habibi,
Volume 14, Issue 3 (3-2015)
Abstract
Background: Alpha-amylase is the most important enzyme in the digestion of starch. Activators of this enzyme could be potentially used as digestive aids and its inhibitors block the absorption of starch compounds and result in the control of blood sugar levels. This study aimed at the investigation of aromatic compounds on bovine serum alpha-amylase. Methods: Effect of carvacrol, cumyl phenol, tryptamine, tryptophan, N-acetyl-L-tryptophan, Bis phenol A, 2-benzyloxy phenol, 2,6 diisopropyl phenol and 4-chloro-2-isopropyl-5-methyl phenol was investigated on bovine serum alpha-amylase with use of artificial substrate (laboratory kit). Results: Most of tested aromatic compounds showed a similar pattern. All these compounds had 5-30% inhibitory effect on the tested serum with the exception of tryptamine which showed a 20% increase in the enzyme activity. The best inhibitory effect was obtained from cumyl phenol in the range of 30%. Conclusion: This study showed that aromatic compounds with one and two cycles have moderate inhibitory effect on bovine serum alpha-amylase and tryptamine showed a slight activator effect. With regard to these results, indolic and phenolic structures may be effective on alpha-amylase, and in the next step, investigation of these compounds derivatives is suggested.
Masoumeh Habibian, Mearaj Khosravi,
Volume 15, Issue 2 (1-2016)
Abstract
Background: Regular exercise training is an effective nonpharmacological strategy in the prevention of mortality and morbidity due to cardiovascular disease in patients with diabetes. Therefore, the aim of this study was to assess the effect of 8 weeks regular swimming exercise intervention on the cardiac levels of Matrix mettaloproteinase-2(MMP-2) and transforming growth factor-β1 (TGF- β1) in diabetic rats.
Methods: Twenty eight male Wistar rats with were randomly divided into 4 groups (7 rats per group) of control, diabetes, exercise, diabetes-exercise. Diabetes induced whit alloxan (90 mg/k g, intraperitoneally, ip) in rats. The animals exercised by swimming training at 5 min to 30 min per day, five days a week over 8 weeks. The rats were killed 72 h after the last treatments and cardiac MMP-2 activity and TGF- β1 levels were evaluated by Zymography and ELIZA method. A one-way analysis of variance was used to data analysis (P<0.05).
Results: The results showed that the inducing of diabetes resulted in a significant increase in the levels of cardiac TGF-β1 (P<0.001) and a decrease in the MMP-2 activity (P<0.001) compared with control group. Furthermore 8 weeks swimming training significantly attenuated the levels TGF-β1 (P=0.001) and normalized MMP-2 activity (P=0.005) in trained diabetic rats.
Conclusion: It seems that regular swimming exercise may have great therapeutic potential in the treatment of diabetes-induced cardiac injury by suppressing elevation the levels of TGF-β1 and upregulation of MMP-2 activity.
Hamed Rezaei Nasab, Roohollah Ranjbar, Abdolhamid Habibi, Mohammad Taher Afshoon Pour,
Volume 17, Issue 1 (3-2018)
Abstract
visfatin secreted by various factors, including cytokines such as TNF-α and IL-6 is affected. The purpose of this study was to evaluate the effect of eight weeks of combined training (resistance circular - aerobic) visfatin concentration, IL-6 and TNF-α in obese men with type II diabetes.
Methods: The population of the research was all men with diabetes type II in District 2 of Ahwaz. Twenty four patients with diabetes type II, aged 40-55 years and fasting blood glucose lower than 200 mg /dl were selected by purposive sampling. First, the anthropometric variables and body composition were measured, then subjects were randomly divided into two groups (experimental, n=12 and control, n=12). Combined exercise training was done for 8 weeks (5 days/week, 30 to 50 minutes) for experimental group. To analyze the changes in the covariance analysis and dependent t test measured variables at a significance level of p≤05.
Result: After eight-week combined exercise training, fasting blood glucose and plasma visfatin significantly decreased p≤05. But no significant changes were observed in the levels of IL-6 and TNF-α p≥05. Also, the inverse relationship between plasma visfatin with IL-6 and TNF-α was observed (P≤ 0.05).
Conclusion: Eight weeks of combined training despite a positive effect on plasma visfatin, due to no significant changes in plasma IL-6 and TNF-α in patients with type 2 diabetes, it seems this type of exercise in reducing obesity and visceral fat and thereby reducing plasma visfatin is appropriate, but the potential change in IL-6 and TNF-α does.
Hamed Rezaei Nasab, Abdolhamid Habibi, Masoud Nikbakht, Mohamad Rashno, Saeid Shakerian,
Volume 18, Issue 4 (4-2019)
Abstract
Background: Oxidative stress plays a key role in the onset and development of diabetes Complications, Including diabetic cardiomyopathy. The purpose of this study was to investigate the role of dichloroacetate (DCA) on SOD and GPX expression following six weeks’ endurance training in cardiac muscle of diabetic male rats.
Methods: In this experimental study, 64 male Wistar rats were selected and randomly divided into eight groups after streptozotocin (STZ) solution diabetic treatment. The endurance training protocol was performed on a treadmill for 6 weeks. In the present study, for Inhibition of PDK4 in the cardiac muscle, intraperitoneal injection of DCA of 50 mg/ kg body weight was used. Gene expressions were measured by Real-Time PCR method. One-way ANOVA and Tukey's test were used to analyze the data.
Results: The results of the study showed that after endurance training, PDK4 gene expression increased and SOD and GPX genes expression in training endurance + diabetic group and endurance training group decreased compared to control group (P <0.05). By Inhibition of PDK4, the of SOD and GPX genes expression increased in DCA + training endurance + diabetic group and DCA + endurance training group compared to control group (P <0.05).
Conclusion: According to the results of this study, DCA injections may reduce the recurrence of free radicals induced by endurance training in diabetic patients by mitochondrial adaptation. Which can reduce the oxidative stress in the heart tissue of diabetic patients and increase cardiac efficiency.
