Showing 2 results for Jahani
Neda Aghaei, Mohammad Sherafati Moghadam, Farhad Daryanoosh, Saeedeh Shadmehri, Shiva Jahani Golbar,
Volume 18, Issue 3 (3-2019)
Abstract
Background: The mTORC1 pathway is one of the important pathways for protein synthesis in the heart, which can lead to physiological or pathological hypertrophy. Diabetes can lead to defects in this pathway. The aim of this study was to examine the effect of 4 weeks’ aerobic training on the content of mTORC1 signaling pathway proteins in heart tissue of type 1 diabetes rats.
Methods: In this experimental study, 16 Sprague-Dawley male rats (mean weight of 300 ± 20 gr) were selected and after induction of diabetes by STZ was randomly assigned into two groups: diabetic training and diabetic control. The experimental group performed HIIT training for 4 weeks’ accordance with the training program (each session 42 minutes, 10-20 m/m) for 4 weeks, while the control group did not have any training program. Dependent t-test and independent T-test were used to analyze the data
Results: Significant increase was observed in the content of AKT1 (p<0.015), mTOR (p<0.001), P70S6K1 (p<0.006), 4EBP1 (p<0.05) proteins in the aerobic training group compared to control group.
Conclusion: Aerobic training for 4 weeks enabled to activate the pathway AKT1/mTOR/P70S6K1 and AKT1/mTOR/4E-BP1 in mTORC1 pathway; therefore, due to cardiac complications in type 1 diabetic patients, aerobic training can lead to protein synthesis and physiological cardiac hypertrophy through mTORC1 pathway.
Majid Jahani, Hasan Matinhomaie, Parvin Farzanegi,
Volume 19, Issue 4 (4-2020)
Abstract
Background: Physical activity plays a major role in the prevention of cardiovascular disease and diabetes, but the effect of intense activity on endoplasmic reticulum proteins and apoptosis and necroptosis in diabetic conditions is unclear. The aim of the present study was to investigate the changes of PERK and CHOP proteins in endoplasmic reticulum of cardiac myocytes of diabetic Wistar rats following continuous and interval exercise.
Methods: For this purpose, 32 male white wistar were purchased and were randomly divided into 4 groups of hemogenus 8 rats in each group: Healthy control (C), Diabetic control (D), Diabetic with moderate-intensity continuous training intensity at the 55min on 26 m/min speed (D+MICT) and Diabetic with high-intensity interval training intensity at the 85-90% of maximum speed (D+HIIT); 5 days/week for 8 weeks. For evaluate changes in the expression of the proteins associated with apoptosis and necroptotic death in the diabetic heart muscle myocardium, based on Western blot analysis will be used. Also, the one-way analysis of variance (ANOVA) is used to determine differences between the study groups.
Results: The results showed that induction of type 2 diabetes increased apoptotic and necroptosis cell death (P≥0.05). Therefore, both continuous and intermittent aerobic exercise modulate apoptotic cell death. And both intermittent and continuous exercise had a significant effect on cell necroptosis death.
Conclusion: It seems that different levels of aerobic exercise have different effects on cardiac myocytes cell death in diabetic rats. But more research is needed to confirm the death of diabetic necroptics.
