Roya Kolahdouz Mohammadi, Mohammad Javad Hosseinzadeh-Attar, Mina Kolahdouzmohammadi, Mohammad Reza Eshraghian, Elnaz Khorrami, Alireza Esteghamati,
Volume 13, Issue 3 (3-2014)
Abstract
Background: CoQ10 is a lipid-soluble and a powerful antioxidant. Decreased level of this antioxidant
was reported in many conditions like vascular, diabetes, high blood pressure and coronary artery
diseases. With regards to increased oxidative stress in diabetes and its role in the development of high
blood pressure, this study aimed to examine the effect of Q10 supplementation on blood pressure level
and its relation to nitric oxide metabolites (NOx) level in type 2 diabetic patients.
Methods: In this 12-week randomized controlled trial, T2D subjects received either placebo or
coenzyme Q10 (100 mg twice a day). Anthropometric measures, blood pressure, biochemical analysis
including NOx level, fasting blood glucose, glycosylated hemoglobin (HbA1c) and lipid profile were
evaluated at the beginning and after the intervention.
Results: The intervention resulted in a significant improvement in systolic blood pressure
(115.3±27.61 versus 118.2±12.6 mmHg), diastolic blood pressure (77.1±8.22 versus
80.3±12.11mmHg), NOx (Pvalue=0.014) and HbA1c. Moreover Q10 supplementation resulted a
significant decrease in elevated levels of cholesterol.
Conclusions: In conclusion, CoQ10 supplementation (200 mg/day) for 12 weeks, significantly
decreased systolic and diastolic blood pressure, NOx, HbA1c, total cholesterol and LDL-C in type 2
diabetic patients.
Kamyar Khoshnevisan, Sayed Mahmoud Sajjadi-Jazi, Farnoush Faridbod, Bagher Larijani, Mohammad Reza Khorramizadeh,
Volume 19, Issue 5 (6-2020)
Abstract
Background: A novel nanocomposite-modified electrode based on reduced graphene oxide (rGO) decorated with crown-ether and gold nanoparticles (GNPs) on the surface of a glassy carbon electrode (GCE) was fabricated to investigate 5-HT determination.
Methods: The morphology of nanocomposite was characterized by scaning electron microscopy (SEM). Diabetic zebrafish was obtained by overfeeding via glucose. 5-HT was successfully determined in the presence of dopamine (DA), ascorbic acid (AA), urea, glucose, and L-tryptophan (L-Trp) by using electrochemical methods.
Results: The nanocomposite exhibited satisfactory electrochemical catalytic activity for 5-HT determination using square-wave voltammetry (SWV). The electrochemical behavior of 5-HT at the nanocomposite/GCE displayed reasonable oxidation current and potential. The limit of detection (LOD) of 5-HT obtained from the real samples, containing the control and diabetic group by using the proposed system and HPLC method, was calculated to be about 0.3 and 0.1 µg/L, respectively. The prposed system also demonstrated high selectivity, reasonable sensitivity, and good stability and reproducibility for 5-HT sensing. The nanocomposite was applied for the determination of the biomarker 5-HT in the diabetic and control groups of zebrafish and displayed excellent recoveries about 93 and low relative error about 3% while compared with standard method.
Conclusions: It seems that the 5-HT level can be used for earlier diagnosis of diabetes.
