Showing 4 results for Mirzaeezadeh
Zahra Mirzaeezadeh, Hajar Zarei, Kobra Omidfar,
Volume 13, Issue 3 (3-2014)
Abstract
Background: In this study, using anti-human serum albumin (HSA)-conjugated gold nanoparticles
(AuNPs) as an electrochemical label and mobile crystalline material-41 (MCM-41)–polyvinyl alcohol
(PVA) mesoporous nanocomposite as an immobilization platform, a new immunosensor was
established.
Methods: Field emission scanning electron microscopy (FESEM), cyclic voltammetry (CV), and
differential pulse voltammetry (DPV) have been applied to determine the physicochemical and
electrochemical properties of this hybrid film in immunosensor development.
Results: A suitable attachment between HSA and MCM-41 and also a thick layer deposition of
MCM-41–HSA–PVA film onto the electrode surfaces was observed by FESEM. DPV was employed
for quantitative determination of antigen.
Conclusion: In optimal conditions, this immunosensor could detect HSA in a high linear range (0.5–
200 µg ml
-1
with a low detection limit of 1 ng ml
-1
. This new strategy showed acceptable
reproducibility, stability, and reliability and could also be applied to detect the other antigens.
Maryam Mortezaee, Abbasali Raz, Shole Mansouri, Zohreh Annabestani, Zahra Mirzaeezadeh, Bagher Larijani, Mehrdad Hashemi, Kobra Omidfar,
Volume 15, Issue 5 (7-2016)
Abstract
Background: Insulin resistance and progressive β-cells failure are the key factors in type 2 diabetes mellitus (T2DM) pathogenesis. Many studies support a primary role of RBP4 in insulin resistance and suggest that genetic variations which alter the expression level of RBP4 might influence the risk of T2DM and its complications. Diabetic foot is one of the main complications of diabetes leading to disability and hospitalization. In addition, it reduces quality of life and imposes great cost to patients. The purpose of this study was to evaluate the correlation between two single nucleotide polymorphisms (rs10882273 and rs10882283) of RBP4 genes with diabetic foot ulcer in order to identify a biomarker for prediction of diabetic foot ulcer.
Methods: This is a case-control study. Two single nucleotide polymorphisms of RBP4 genes were genotyped by hit Tetra ARMS PCR technique. In this study, 100 and 133 diabetic patients with and without foot ulcer were selected as the cases and controls, respectively.
Results: The Chi-square test revealed no significant difference in frequency of TT, CC and TC alleles of rsl0882273 between case and control groups (P=0.414). Also, Comparison of AA, CC and AC alleles of rsl0882283 in both groups did not show significant difference (P=0.85).
Conclusion: According to this study, there is no relationship between two single nucleotide polymorphisms of RBP4 genes (rs10882273 and rs10882283) with diabetic foot ulcer in type2 diabetes patients.
Sharare Hassanzade, Abbasali Raz, Masoumeh Mansouri, Zahra Mirzaeezadeh, Bagher Larijani, Mehrdad Hashemi, Kobra Omidfar,
Volume 16, Issue 1 (1-2017)
Abstract
Background: Type 2 diabetes is a metabolic disorder characterized by high blood sugar levels that can damage nerves. Many organs are affected ،especially the foot that leading to loss of sensation. These factors make favorable conditions for the development of diabetic foot ulcers. Polymorphisms (Thr399Ile) of Toll Like Receptor4 (TLR4) gene due to malfunction of TLR4 protein which plays an important role in immunity. The purpose of this study was to determine the parameters which are affecting the imbalance resulting in chronic inflammation and wound healing. By showing the relationship between single nucleotide polymorphism (Thr399Ile) of TLR4 gene with diabetic foot ulcer we can identify a biomarker for prediction of diabetic foot ulcer.
Methods: This is a case-control study. Single nucleotide polymorphisms of TLR4 gene were genotyped by hit Tetra ARMS PCR technique. In this study, 100 and 120 diabetic patients with and without foot ulcer were selected as the cases and controls, respectively.
Results: The Chi-square test revealed significant difference in frequency of TT, CC and TC alleles of (Thr399Ile) between case and control groups (P=0.021).
Conclusion: According to this study, there is a relationship between single nucleotide polymorphisms (Thr399Ile) of TLR4 gene with diabetic foot ulcer in type 2 diabetes patients
Sahar Shafiee, Abbasali Raz, Neda Adibi, Masoomeh Mansouri, Zohreh Annabestani, Zahra Mirzaeezadeh, Mehrdad Hashemi, Kobra Omidfar,
Volume 17, Issue 1 (3-2018)
Abstract
Background: Type 2 diabetes is the most common type of diabetes, and a complex endocrine metabolic disorder that one of its main complications is diabetic foot ulcers. Matrix metalloproteinases (MMPs) are one of the key enzymes in the reconstruction of extracellular matrix which have proteolytic activity. The aim of this research is based on evaluating relationship between -1562 C>T allele at MMP-9 gene promoter with diabetic foot ulcer in type II diabetic patients. If such correlation proves, it can be used as a prognostic biomarker in patients with high-risk.
Methods: This is a case-control study. The single nucleotide polymorphism of -1562C>T allele of MMP9 gene promoter was genotyped by hit Tetra ARMS PCR technique in 100 diabetic patients with foot ulcer grade 1 or 2 as the case group according Wagner classification and in 100 diabetic patients without foot ulcer as the control group. Results: The Chi-square test revealed significant difference in genotype frequency of CC, CT and TT alleles of -1562C>T allele of MMP9 gene promoter between case and control groups (P=0.000).
Conclusion: According to this study, there is a relationship between -1562C>T allele of MMP9 gene promoter with diabetic foot ulcer in type2 diabetes patients. Thus we can introduce this biomarker for evaluation of risk and prognosis of diabetic foot ulcers.
