Showing 4 results for Mohsenzadeh
Fatemeh Soltanmohammadi, Mahsa Mohsenzadeh, Fuad Feizollahi,
Volume 19, Issue 2 (1-2020)
Abstract
Background: The aim of the present study was to investigate the effect of 8 weeks of HIIT training and supplementation of black grape seed extract on Wnt and Β-catenin gene expression in pancreatic tissue in male rats with type 2 diabetes.
Methods: In this experimental study, 40 male Wistar rats, with average weight of 250 ± 20 gr, after induction of diabetes by STZ, were randomly divided into 5 groups: training- supplement, training-placebo, supplement and diabetic-control and base control. After becoming diabetic, rats were familiarized with protocol for one week and then trained for eight weeks, five days a week. They ran on treadmill with 90% of VO2 max. Intragastric injection of GSPE extracted from black grape seed (Cabernet sauvignon) once a day for 8 weeks (250 mg/kg, proanthocyanidin content >96%). 24 hours after the last session of training, pancreas tissue was extracted, and the level of gene expression was measured by Real Time-PCR. In all statistical analyzes, the statistical significance level was considered to be 0.05.
Results: Eight weeks and supplement significantly increased the mean expression of Wnt and Β-catenin genes in pancreatic tissue of type 2 diabetic rats but the effect of exercise with black grape seed extract on the expression of Wnt genes in the pancreas of diabetic rats was not significant.
Conclusion: It seems that regulating the expression of Wnt and Β-catenin genes through exercise and consumption of black grape seed extract is likely to improve and maintain the function of pancreatic beta cells, especially in diabetic samples.
Bahare Ghani, Mahsa Mohsenzadeh, Foad Feyzollahi,
Volume 19, Issue 5 (6-2020)
Abstract
Backgrond: Although some studies have studied the mechanism of action of beta cells in animal models and more or less in human populations, but so far the role of exercise therapy or exercise exercise HIIT along with black grape supplementation on the expression of involved genes Less has been studied in pancreatic beta cells, so the aim of the present study was to investigate the effect of eight weeks of intense intermittent exercise with black grape supplementation on the expression of PPARα and PPARγ gene in pancreatic tissue of male rats with type 2 diabetes.
Methods: The study was conducted in the form of an experimental design. The subjects of this project were 40 8-month-old male rats with an average weight of 250 grams. After familiarizing the subjects with exercise and induction of diabetes by STZ, the subjects were randomly divided into 5 groups including exercise, supplement, exercise and supplement, diabetic control and basic control. After 8 weeks of training, 5 sessions per week for 8 weeks with 90% Vo2max activity and supplementation of black grape seed extract, PPARα and PPARγ genes were measured after RNA extraction from pancreas and cDNA synthesis. PPARα and PPARγ genes were measured by Time-Real PCR. Data were analyzed using independent t-test and two-factor analysis of variance at a significance level of 0.05 by SPSS software version 24.
Results: The effect of exercise with consumption of black grape seed extract on the expression of PPARγ and PPARα genes in the pancreas of diabetic rats was not significant, but exercise alone significantly increased the expression of PPARα gene in the pancreatic tissue of diabetic specimens. Consumption of black grape seed extract alone significantly increased the expression of PPARγ and PPARα genes.
Conclusion: It seems that regulating the expression of PPARα and PPARγ genes through exercise and consumption of black grape seed extract may lead to the improvement and maintenance of pancreatic beta cell function in diabetic rats.
Syed Reza Mir Javadi, Alireza Rahimi, Fariba Aghaei, Mahsa Mohsenzadeh,
Volume 21, Issue 5 (1-2022)
Abstract
Background: Because insulin therapy cannot properly control the progression of diabetes and its complications, other alternative therapies may be desirable. The aim of this study was to investigate the effect of resistance training and endothelial stem cell injection on skeletal muscle oxidant and antioxidant status in STZ-induced diabetic male rats.
Method: In this experimental study, 36 male Wistar rats (age 6 weeks) were divided into six groups of control (healthy), basal diabetic control, diabetic control, diabetes + stem cell injection, diabetes + resistance training and diabetes + stem cell injection + resistance training. In this study, rats became diabetic intraperitoneally using streptozotocin as a single dose of 40 mg/kg. Resistance exercises including climbing a one-meter ladder with weights hanging from the tail were performed for 17 sessions. 500,000 bone-derived stem cells were injected by a cell counter. The levels of superoxide dismutase (SOD) and malondialdehyde (MDA) in skeletal muscle tissue of rat were measured by using the kit and ELISA method.
Results: The results showed that the SOD level of rats in the resistance training and endothelial stem cell injection group was significantly higher than the diabetic control group (P<0.001). Also, the level of MDA rats in the resistance training and endothelial stem cell injection group was significantly lower than the control group (P<0.001).
Conclusion: Resistance training and endothelial stem cell injections can be considered as a non-pharmacological treatment to reduce skeletal muscle complications in type 1 diabetes.
Suren Valafar, Eydi Alijani, Fariba Aghai, Mahsa Mohsenzadeh,
Volume 22, Issue 2 (7-2022)
Abstract
Background: Diabetes, as a progressive disease, can lead to decreased immune function. therefore, the aim of this study was to determine the simultaneous effect of resistance training and endothelial progenitor cell injection on immunoglobulins (IgA, IgM, IgG) of streptozotocin-induced diabetic rats.
Methods 30 rats (aged 6 weeks with a mean weight of 200±20 g) were randomly divided into groups including Diabetes + stem cell injection + resistance training (n = 6), diabetes + resistance training (n = 6), diabetes + stem cell injection (n = 6), control diabetes (n = 6) and healthy basal (n = 6) Were divided. Western blotting was used to evaluate the changes in immunoglobulins. Also, two-way analysis of variance was used for comparison between and within the group, and for better understanding of the results, the effect size, and the amount of 95% confidence interval were given.
Results: The results showed that IgA (P = 0.022), IgM (P = 0.017), IgG (P = 0.045) had significant changes between groups. Also, there was a significant difference in all three variables between the control diabetes group and the diabetes + resistance training + injection group (P≤0.05).
Conclusion: Summarizing the results of the present study, it can probably be said that resistance training and simultaneous injection of endothelial progenitor cells improve the status of immunoglobulins by training and injection. These findings suggest that resistance training and injections can be used as a treatment to improve the function of the immune system due to diabetes.
