Iranian Journal of

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Background: The purpose of this study was to investigate the effects of endurance training on muscle NHE1 and NBC1 gene expressions in type 2 diabetic rats. Methods: Male wistar rats (n=40), 4weeks old and 93.7±9.8g, were randomly selected and divided into control, diabetic control and diabetic training groups. The Endurance training was performed for 7 weeks on diabetic training groups (running on treadmill forrodent). NHE1 and NBC1 gene expression were determined by Realtime-PCR technique. The differences between groups in variables were determined by an independent t-test using REST Software. Results: NHE1 mRNA expression reduced significantly in EDL and Soleus by 25% and 19% in the diabetic control group compared with the control group, respectively (P<0/05).NHE1 mRNA expression also reduced significantly in EDL and Soleus by 35% and 29% in the diabetic control group compared with the control group, respectively (P<0/05).Endurance training increased NHE1 and NBC1 geneexpressions in both EDL and Soleus in the diabetic training group. Conclusion: The present study showed that NHE1 and NBC1 mRNA expressions decreased significantly in the diabetic control group and endurance training increased NHE1 and NBC1 mRNA expressions in the diabetic trained group leading to normalizing the mRNAs in diabetic trained group.

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Background: The purpose of this study was to investigate the effect of concurrent training on pre-inflammatory (IL-1β, TNF-α) and anti-inflammatory (IL-10) cytokines gene expression in women with type2 diabetes.
Methods: 18-patients (age30-38, >130 glycemic index) were selected, randomly, and divided into control (n=6) the concurrent training(diabetes, n=6) and the concurrent training (healthy, n=6)  groups. The concurrent training protocol consisted of 3 sessions resistance training per week, 8sets with 80% one maximum repetition( and the endurance training preformed with 30- minutes running (3sets×10 minutes) on a treadmill with 70-80 maximum heart rate(70-80 MHR), immediately. The leukocyte^’s IL-1β, TNF-α and IL-10 genes expression determined by the Real time-PCR technique. The quantitative expression of the cytokines gene was calculated using the 2^{-ΔΔ CT} method. The between- groups differences in variables were determined by independent t-test (permutation test) through REST software and independent one-way ANOVA.
Results: The results showed that IL-1β, TNF-α mRNA genes expression reduced significantly after the concurrent training in both the training groups in comparison to the control group (P<0.05). In addition, the results also showed that IL-10 mRNA gene expression was not expressed in leukocytes after the concurrent training in both training groups in comparison to the control group (P>0.05).
Conclusion: In conclusion, the results suggest that the concurrent training modulate IL-1β and TNF-α mRNA genes expression significantly in diabetic women but could not change IL-10 genes expression. This type of exercise training seems to be more effective in reducing the expression of pro-inflammatory cytokines than in enhancing anti-inflammatory cytokines.