Seddigheh Asgari, Gholamali Naderi, Mojgan Gharipour, Gholamreza Dashti , Ali Sajadian,
Volume 3, Issue 1 (16 2004)
Abstract
New studies have introduced atherosclerosis as an inflammatory disorder. Usage of anti-inflammatory drugs thus can be useful in preventing atherosclerosis and anti-inflammatory herbal medicines without side effects seem desirable in this view.
Amirkahiria Odorutissima (AO) (umbelliferae) is an Iranian flora being traditionally used a:> an antiinflammatory drug. This study assessed the effects of this plant on the development and progression of fatty streaks.
Methods: Twenty male rabbits were obtained from Iran Pasture institute and were kept under standard condition for two weeks, then randomly divided into four groups with different diets. Groups 1 were fed on rabbit chow (normal cholesterol), group 2 were fed on a high cholesterol diet. group 3 were fed on normal cholesterol diet supplemented with AO, group 4 were fed on high cholesterol diet supplemented with AO. After 12 weeks, the animals were killed and biopsied for their aortas and right and left branches of coronary arteries. Biochemical factors were measured at baseline and after 12 weeks. Levels of cholesterol, LDL, HDL. triglyceride and fasting blood sugar (FBS) were determined by enzymatic methods, and quantitative CRP were determined with turbidometrie methods, malondialdehyde (MDA) and antioxidant capacity were determined with spectrophotometry.
Results: The results indicated that there was a significant difference between AO supplemented group compared with other groups (p<0.05) in terms of mean grade o"' fatty streak o"' right and left coronary arteries and aorta. At the end of the 12 weeks period, cholesterol. LDL and CRP were significantly reduced in the groups 3 and 4 (p<0.05).
Conclusion: This study suggests a protective role for Amirkahiria odoratissima Mozaffahan against the development of fatty streak the mechanism of which should be investigated in future studies.
Bagher Larijani, Seyed Sajad Mohseni Salehi, Shirin Irani, Marjan Akbari Kamrani, Nasim Sheykh Bahaei, Ahmad Sajadi, Seyed Naser Ostad,
Volume 6, Issue 1 (19 2006)
Abstract
Background: Many researches have been conducted on islet cells' transplantation for a definitive treatment of diabetes mellitus type1. As the viability of the islets is the most important factor in predicting the transplantation prognosis, we have designed a study to isolate rat's islets. The aim of the study was to assess the viability of the islets at different stages and suggest the best transplantation time.
Methods: Pancreatic islets were isolated from male rats (250-300gr) by standard surgical procurement followed by intraductal HBSS distension, chopping and digestion with collagenase (type V). After being centrifuged for 3 times, the islets were then hand-picked and incubated in 37oC with RPMI 1640 media for 6 days. Each well contained 35-45 islets. Viability of islets was assessed by 2 independent investigators, giving score 0-2 to the color of islets under florescent microscope after Propidium iodide/Acridine orange staining at 6 times: just after the incubation, 24h, 48h, 3rd day, 5th and 6th day.
Results: The viability of the islet cells was gradually increased after the incubation as we had the most viability rate after the second day, while it decreased after this period and reached the least rate on the 5th and 6th day.
Conclusion: The islets' viability increased following the cell culture after the isolation procedure, as they have the best condition for transplantation after 48 hours. As the islets’ viability is the most critical point in transplantation, further studies evaluating the effects of different interventions on viability is needed.
