Showing 5 results for Sarshin
Hadis Jafari Sohi, Eidy Alijani, Amir Sarshin, Fariba Aghaei,
Volume 21, Issue 3 (9-2021)
Abstract
Background: Exercise and the simultaneous use of progenitor cells is a new strategy aimed for reducing diabetic disorders. One of the known mechanisms is angiogenic disorders caused by diabetes. Therefore, the present study was performed to determine the simultaneous effect of resistance training with endothelial progenitor cell injection on the expression of angiogenic factors in the skeletal muscle of diabetic rats.
Methods: In this study, 30 rats were randomly divided into 5 groups: healthy, diabetic control (D) trained diabetic (DR), diabetic with endothelial progenitor cell injection (DI), diabetic trained with endothelial progenitor cell injection (DRI) were divided. VEGF protein expression was measured by Western blotting and insulin resistance index was measured by ELISA. The data were analyzed using two-factor analysis of variance test with SPSS software version 19 at a significance level of 5%.
Results: In this study, 6 weeks of resistance training or progenitor cell injection caused a significant increase in VEGF and a significant decrease in insulin resistance index in diabetic rats. In the group that used simultaneous exercise and injection compared to the group with exercise and injection and these changes were significant in the group of simultaneous use of exercise and injection compared to the group of exercise with injection.
Conclusion: Based on the results of this study, it can be stated that resistance training or injection of endothelial progenitor cells can stimulate angiogenesis in skeletal muscle, also the simultaneous use of these two factors is a better way to increase angiogenesis in rats
Ali Asghar Soleymani, Alireza Rahimi, Eidi Alijani, Amir Sarshin,
Volume 21, Issue 6 (3-2022)
Abstract
Background: Cardiovascular disease is a major cause of death in patients with type 1 diabetes, and cardiovascular risk remains high even in patients with type 1 diabetes with good metabolic control. The aim of this study was to evaluate the effect of six weeks of aerobic exercise on inflammation and damage indicators of heart tissue in type 1 diabetic male rats.
Methods: In this experimental study, 19 male Wistar rats (mean weight 200-250 g) were randomly divided into four groups: aerobic training, sham, control and healthy. In this study, induction of type 1 diabetes was performed by injecting a single dose of streptozotocin dissolved in sodium citrate buffer intraperitoneally. Aerobic exercise program was performed with intensity of 50-60% VO2max, 5 days a week for 6 weeks. After anesthesia, an autopsy was performed and left ventricle of the heart was removed. Levels of Tumour necrosis factor α (TNF-α), Peroxisome proliferator-activated receptor-γ coactivator (PGC-1α) and Creatine kinase (CK) in rat heart tissue were measured by Western blotting. Data were analyzed by One-way ANOVA and Tukey post hoc test at the P<0.05.
Results: The results showed that six weeks of aerobic training led to significant decrease in TNF-α and CK and significant increase in PGC-1α of the heart tissue in type 1 diabetic rats (P<0.001).
Conclusion: According to the results, it seems that aerobic training can help improve the inflammation and damage indicators of heart in type 1 diabetes.
Hadis Jafari Sohi, Eidy Alijani, Amir Sarshin, Fariba Aghaei,
Volume 21, Issue 6 (3-2022)
Abstract
Background: Angiogenesis disorders are known mechanisms of diabetes. With the aim of reducing angiogenesis disorders, resistance training and its combination with endothelial progenitor cell injection are new strategies. Therefore, the present study was performed to determine the effect of resistance training with endothelial progenitor cell injection on the expression of angiogenic factors in the skeletal muscle of diabetic rats.
Methods: In this study, 30 rats were randomly divided into 5 groups: healthy, control (D) diabetic, trained diabetic (DR), endothelial progenitor cell (DI) diabetic, trained endothelial progenitor cell (DRI) diabetic) Were divided. Ang1 and Tie2 protein expression changes were measured by Western blotting. Data were analyzed using two-factor analysis of variance with SPSS software version 19 at a significance level of 5% α≤.
Results: In this study, 6 weeks of resistance training led to a significant increase in Ang1 and Tie2 proteins. But injection of endothelial progenitor cells was significant only on the amount of Tie2 protein. The interactive effect of resistance training and endothelial progenitor cell injection was significant only on the amount of Tie 2 protein. In other words, the combination of resistance training and endothelial progenitor cell injection was superior to Tie2 protein expression than training or injection alone.
Conclusion: It can be said that resistance training improves angiogenesis in diabetics. Combining resistance training with endothelial progenitor cell injections could possibly stimulate angiogenesis in skeletal muscle and be a new strategy in the treatment of diabetic disorders.
Elham Mokhtari, Amir Sarshin, Foad Feizolahi, Eidi Alijani,
Volume 22, Issue 2 (7-2022)
Abstract
Background: Type 1 diabetes is associated with decreased skeletal muscle capillary and improper regulation of angiogenesis pathways in skeletal muscle. This research intended to study the effect of resistance training and endothelial stem cell injection on βeta-actin, phosphorylated and total AKT of skeletal muscle in type 1 diabetic rats.
Methods: In this experimental study, 36 male Wistar rats (age 6 weeks) were divided into six groups of control (healthy), basal diabetic control, diabetic control, diabetes + stem cell injection, diabetes + resistance training and diabetes + stem cell injection + resistance training. In this study, rats became diabetic intraperitoneally using streptozotocin as a single dose of 40 mg/kg. Resistance exercises including climbing a one-meter ladder with weights hanging from the tail were performed for 17 sessions. 500,000 bone-derived stem cells were injected by a cell counter. The levels of βeta-actin, phosphorylated and total AKT in skeletal muscle tissue of rat were measured by using the Western blotting method.
Results: The results showed that resistance training led to significant increase in Pho-AKT, β-actin and Pho-AKT/AKT ratio and significant decrease in AKT of muscle tissue in type 1 diabetic rats (P<0.001). Injection of stem cells leads to significant increase in Pho-AKT and Pho-AKT/AKT ratio and resistance training with simultaneous injection of stem cells leads to significant increase in Pho-AKT, β-actin and significant decrease in Akt of muscle tissue in type 1 diabetic rats (P<0.001).
Conclusion: According to the results, it is possible that the intervention of resistance training with injection of stem cells can help regulate the pathways of skeletal muscle angiogenesis in type 1 diabetes.
Morteza Chenari, Alireza Rahimi, Amir Sarshin, Foad Feizolahi,
Volume 22, Issue 2 (7-2022)
Abstract
Background: Damage to the heart tissue in diabetics causes inflammation and destruction of heart cells, which in turn leads to apoptosis or cell death. The aim of this study was to investigate compare the effect of six weeks of aerobic and resistance training on apoptotic indice of caspase-8 and catalase in the heart tissue of male diabetic rats.
Method: In this experimental study, 24 male Wistar rats were divided into six groups: aerobic training, resistance training, aerobic sham, resistance sham, control and healthy. Diabetes was induced by intraperitoneal injection of a single dose of streptozotocin in the amount of 30 mg per kg. The aerobic and resistance training program was performed for six weeks. Western blotting was used to measure caspase-8 and catalase. Data were analyzed by one-way analysis of variance and Tukey post hoc test at the P<0.05.
Results: The results showed that the mean difference of caspase-8 between aerobic training group and healthy group (P=0.752), resistance training group with healthy group (P=0.723) and resistance training with aerobic training group (P=1.00) were significant. Caspase 8 was lower in the aerobic exercise group than in the resistance exercise group. The difference between the mean catalase between the aerobic training group with the healthy group (P=0.024) and the aerobic training group with the resistance training group (P=0.023) was significant and the amount of catalase in the resistance training group was higher than aerobic training.
Conclusion: Aerobic and resistance training can reduce the apoptotic index of caspase-8 and increase catalase in the heart tissue of diabetic rats.
