Showing 6 results for Yaghmaei
Maryam Chinisaz, Azadeh Ebrahim-Habibi, Parichehreh Yaghmaei, Kazem Parivar, Ahmad- Reza Dehpour,
Volume 13, Issue 4 (5-2014)
Abstract
Background: The flexible structure of proteins is one important factor in the formation of ordered aggregates (amyloid fibril). This is a major problem for therapeutic proteins such as insulin. Study on the induction and inhibition of insulin fibrillation process with specific compounds such as aromatic derivatives may provide useful information about means of stabilization of protein structures.
Methods: To induce fibrillation, regular insulin was incubated in phosphate buffer (pH=7.4) during 24 hours. Amyloid formation was investigated by using Congo red absorbance and transmission electron microscopy (TEM). Then nodular amyloidosis was observed in mice upon amyloid fibril injection, after which the excised nodule was studied by Congo red staining and polarized light microscopy. Then, some aromatic compounds effect was investigated on the fibrillation process.
Results: Regular insulin form mature amyloid fibrils at pH=7.4, 37°C after 24 hours. Silibinin had the highest inhibitory effect on that process. Furthermore, Amyloid fibril injection in mice caused nodular amyloidosis.
Conclusion: Regular insulin has a high potential to undergo amyloid aggregation. Nodular amyloidosis confirms fibril formation by insulin under in vitro condition. Silibinin could be considered as a potential compound capable to increase protein structure stability.
Parichehreh Yaghmaei, Faazaneh Abbasi, Azadeh Ebrahim-Habibi, Atieh Hesaraki,
Volume 13, Issue 5 (7-2014)
Abstract
Background : PCOS (Polycystic Ovary Syndrome = PCOS) is a relatively common disease in women of childbearing age. Bisphenol s are chemical groups that are composed of two functional hydroxyl group and most of them are based on methane. In this study, the effects of phenolic compounds, (bisphenol A and AP) in polycystic ovary syndrome were investigated.
Methods : mature Wister rats were classified in six groups . Healthy controls ( healthy rats that received Grape seed Oil as solvent ), PCOS Group ( disease induced by testosterone propionate ) experimental groups 1.2.3.4 , respectively. after induction of PCOS,they received bisphenol- A and AP in doses of25 mg / kg, 50mg/kg, by gavage . Blood samples were taken and the hormones LH, FSH was measured . Ovaries were also to be studied histologically .
Results : The results showed that the amount of LH to FSH ratio increases after induction with bisphenol-A and bisphenols AP,. Furthermore the number of follicles and growing follicles were reduced significantly, and these concequences causing negative effects on polycystic ovary syndrome.
Mahsa Soltani-Nobakht , Parichehreh Yaghmaei, Azadeh Ebrahim-Habibi,
Volume 14, Issue 3 (3-2015)
Abstract
Background: Alpha-amylase is the most important enzyme in the digestion of starch. Activators of this enzyme could be potentially used as digestive aids and its inhibitors block the absorption of starch compounds and result in the control of blood sugar levels. This study aimed at the investigation of aromatic compounds on bovine serum alpha-amylase. Methods: Effect of carvacrol, cumyl phenol, tryptamine, tryptophan, N-acetyl-L-tryptophan, Bis phenol A, 2-benzyloxy phenol, 2,6 diisopropyl phenol and 4-chloro-2-isopropyl-5-methyl phenol was investigated on bovine serum alpha-amylase with use of artificial substrate (laboratory kit). Results: Most of tested aromatic compounds showed a similar pattern. All these compounds had 5-30% inhibitory effect on the tested serum with the exception of tryptamine which showed a 20% increase in the enzyme activity. The best inhibitory effect was obtained from cumyl phenol in the range of 30%. Conclusion: This study showed that aromatic compounds with one and two cycles have moderate inhibitory effect on bovine serum alpha-amylase and tryptamine showed a slight activator effect. With regard to these results, indolic and phenolic structures may be effective on alpha-amylase, and in the next step, investigation of these compounds derivatives is suggested.
Parisa Dana, Nasim Hayati Rodbari, Parichehreh Yaghmaei, Zahra Haj Ebrahimi,
Volume 21, Issue 4 (10-2021)
Abstract
Background: Empagliflozin, selective glucose-sodium inhibitor of the latest drugs in the treatment of type 2 diabetes. Diabetes induced hypogonadism disrupts sexual function. There is a direct relationship between reducing blood glucose and reduced libido. In this project, the anti-diabetic drug Empagliflozin in addition to treatment has been studied, in terms of effect on sexual function.
Methods: Type 2 diabetes with injection of 60 mg/kg streptozotocin per body weight was create intraperitoneally. The primary diabetes was treated with two doses of 10 and 25 mg/kg Empagliflozin per body weight. Sexual hormones, biochemical parameters and lipid profile were measured. Evaluation of Sperm parameters and morphological studies were performed on testicular tissue, pancreas and epididymis. Data were analyzed by one-way ANOVA in SPSS software version 22 and P˂0.05 was considered as a significant level.
Results: Empagliflozin significantly increases the quality, survival and natural state of sperm head and tail. Empagliflozin reduces weight in diabetic rats. Empagliflozin Without increasing hyperglycemia by reducing blood glucose affects steroidogenesis, maintains fertility strength in testicles, and increases the level of sex hormones. Empagliflozin significantly increases insulin secretion. Empagliflozin maintains and integrity of pancreatic beta cells. Empagliflozin has no effect on lipid profile.
Conclusion: Empagliflozin in addition positive effects on the treatment of diabetes in the early stage improves sexual function in adult rats.
Marayam Arabloei Sani, Parichehreh Yaghmaei, Zahra Hajebrahimi, Nasim Hayati Roodbari,
Volume 22, Issue 3 (9-2022)
Abstract
Diabetes is a serious public health problem in low- and middle-income countries. There is a strong association between hyperglycemia, oxidative stress and the development of diabetes. Therefore, screening and early diagnosis of this disease in people at high risk can be effective in preventing these complications. The main route of insulin signaling is to control lipid and glucose metabolism, and its imbalance leads to type 2 diabetes. P-Cymene is an aromatic monoterpene with a wide range of therapeutic properties including antioxidant activity. The aim of this study was to evaluate the effect of p-Cymene at a dose of 50 mg / kg in preventing the symptoms of diabetes and serum factors.
Materials and Methods: 30 male Wistar rats weighing approximately 250 g were randomly selected and divided into five groups: control, diabetic control (sham), metformin-treated diabetic (met), and non-diabetic treated. P-Cymene and diabetics treated with P-Cymene were divided. One week after streptozotocin injection, treatment with P-Cymene at a dose of 50 mg / kg was performed by sunflower oil solvent for 28 days by gavage. At the end of the experiment, blood samples were taken from the hearts of rats and the parameters of glucose, insulin, and superoxide dismutase (SOD) were measured.
Results: The results showed that diabetes increased water and food intake as well as glucose levels and decreased insulin (P <0.001) and superoxide dismutase activity in rats and administration of P-Cymene extract improved the symptoms of diabetes and oxidative stress in Became diabetic rat.
Hamed Alizadeh Palavani, Mehdi Yaghmaei, Sanam Mirzaee Moghamir, Reza Salboukhi,
Volume 23, Issue 4 (11-2023)
Abstract
Background: Several proteins regulate the autophagy pathway, and one of the most important regulators is the BECLIN family proteins. Therefore, this research aims to investigate the effect of high-intensity interval training on the amount of BECLIN1/2 family autophagy proteins in the left ventricle of the heart of rats with type 1 diabetes.
Methods: In this experimental study, 18 three-month-old male Sprague Dawley rats with an average weight of 300±20 grams were selected. 12 rats were diagnosed with type 1 diabetes through intraperitoneal injection of streptozotocin solution. These rats were randomly divided into two groups, diabetic exercise, and diabetic control; A healthy control group was also considered; The HIIT was performed for 6 weeks and 4 sessions each week including 5 bursts of 4 minutes with an intensity equal to 85-95% of the maximum speed and 3-minute active rest periods with an intensity equal to 50-60% of the maximum speed. Data analysis was done through one-way ANOVA and Tukey's post hoc tests in SPSS version 26 software. A significance level of 0.05 was considered.
Results: The intracellular content of BECLIN1 and BECLIN2 protein showed a significant decrease between the research groups in the left ventricle of the heart after six weeks of HIIT (p=0.0001).
Conclusion: It seems that BECLIN family proteins are decreased by HIIT and this can decrease the autophagy mechanism in cardiac cells.
