Alireza Zandinezhad, Hossein Abednatanzi, Farshad Ghazalian, Alireza Barari,
Volume 23, Issue 3 (9-2023)
Abstract
Background: The aim of the present study was to study the changes in the expression of Cox-1 and Cox-2 genes in liver tissue and tumor volume after aerobic exercise and consumption of pineapple extract in mice with skin cancer.
Methods: This fundamental-laboratory study was conducted on 32 male C57BL/6 mice in four groups including control, aerobic exercise, pineapple extract and aerobic-pineapple exercise. After tumor induction, the animals underwent aerobic training program for six weeks and pineapple extract was gavage at 300mg/kg. The weight and tumor volume of mice were measured. After obtaining blood and tissue samples, expression of Cox-1 and Cox-2 genes in liver tissue was done by RT-PCR method. Then the data were analyzed using one-way analysis of variance, two-factor analysis of variance and post hoc test, and the significance level of p≤0.05 was considered.
Results: Compared to the control group, aerobic exercise and pineapple-exercise showed a significant decrease in tumor volume and a decrease in the expression of Cox-1 (0.59±0.97) and Cox-2 (0.5±0.4) gene expression in the aerobic-Pineapple compared to the control group (1±0) (p≤0.05). Cox1 gene expression increased significantly in the Aerobic exercise group, but a significant decrease was observed in the pineapple-Aerobic group. Also, Cox2 gene expression in the liver tissue in the exercise group Aerobic and interactive Aerobic-pineapple group had a significant decrease compared to the control, but in the pineapple group compared to the control, the difference in the expression of this gene was not significant.
Conclusion: The results of the present study showed that the expression of Cox 1 gene was significantly increased in the aerobic training group and significantly decreased in the pineapple-training group. Cox-2 gene expression in the liver tissue in the aerobic training group and the pineapple-training interactive group had a significant decrease compared to the control, because the Cox-2 enzyme is related to inflammation and pain, angiogenesis, cancer and Alzheimer's disease. Therefore, it can be concluded that its inhibition or reduction can be considered as a promising and effective strategy for the treatment and prevention of cancer.
