Behzad Azadbakht, Abbas Saremi, Mojtaba Khansooz,
Volume 23, Issue 2 (5-2023)
Abstract
Background: The effects of exercise on kidney tissue in withdrawal syndrome are unclear. In this study, the effect of 8 weeks of endurance training on the protein levels of cytochrome C, caspase 8 and caspase 3 in the kidney tissue of diabetic and diabetic rats with withdrawal syndrome was investigated.
Methods: 32 male Wistar rats were used in this experimental study. After the induction of diabetes, the rats were randomly divided into 4 groups of 8. Including: diabetes control (D), morphine diabetes (D.M), diabetes + endurance training (D.ET), morphine diabetes+endurance training (D.M.ET). Then, in addicted groups, the oral method was used for morphine dependence. The training groups performed 8 weeks of endurance training protocol. At the end of the study, all rats were anesthetized, dissected and their kidneys were separated and kept at -70 degrees for further investigations. Protein levels of the indicators of this study were measured by ELISA kits. Data were analyzed using one-way ANOVA and Tukey's post hoc test at a significance level of P≤0.05.
Results: The results of this study showed a significant decrease in caspase 3 protein levels in D.ET (P=0.000), D.M.ET (P=0.000) and cytochrome C levels in D.ET (P=0.000), D.M.ET (P=0.000) groups in Comparison with group D. Also, caspase 8 protein levels in D.M.ET group (P=0.015) compared to D group and D.M.ET group (P=0.038) compared to D.M group showed a significant decrease (P≥0.05).
Conclusion: Endurance training can probably have a positive and protective effect on the reduction of kidney tissue apoptosis in diabetic and diabetic withdrawal syndrome rats.
