Showing 24 results for Type 1 Diabetes
Hamed Alizadeh Pahlavani, Mahya Sharifi Rayeni, Arman Rastegari, Reza Moayedi,
Volume 23, Issue 5 (12-2023)
Abstract
Background: Muscle atrophy is one of the serious complications of type 1 diabetes. Important cellular mechanisms including pathways related to mTOR protein are very important in regulating muscle mass; Therefore, this research was conducted to investigate the effect of high-intensity interval training (HIIT) on the intracellular content of the central protein of mechanical target of rapamycin 1/2 complexes in EDL skeletal muscle of rats with type 1 diabetes.
Methods: In this experimental study, 12 three-month-old male Sprague-Dawley rats with an average weight of 300±20 grams were selected. Type 1 diabetes was induced through intraperitoneal injection of streptozotocin solution (50 mg/kg of body weight). These rats were randomly divided into two groups, diabetic exercise, and diabetic control; The training group performed HIIT for six weeks at an intensity of 85-95% of maximum speed. Data analysis was done through an independent t-test in SPSS software version 28. A significance level of 0.05 was considered.
Results: The intracellular content of total and phosphorylated forms of mTOR protein showed a significant increase after six weeks of HIIT (P=0.0001). Also, the ratio of total to phosphorylated intracellular content of mTOR protein showed a significant increase in the training group compared to the control group (P=0.0001).
Conclusion: HIIT increased the intracellular content of total and phosphorylated forms of mTOR protein, which could possibly lead to protein synthesis and increased muscle hypertrophy.
Akbar Ghodratnama, Mahya Sharifi Rayeni, Arman Rastegari, Reza Moayedi,
Volume 24, Issue 1 (3-2024)
Abstract
Background: Cardiac function in diabetic people is affected through different cellular mechanisms; One of these important cellular pathways is the c-Jun N-terminal kinases (JNKs), whose dysfunction can lead to heart failure; Therefore, the aim of this research is to investigate the effect of moderate intensity interval training on total and phosphorylated content of c-Jun N-terminal kinases protein in heart tissue of type 1 diabetic rats.
Methods: In this experimental study, 12 two-month-old male Sprague Dawley rats with an average weight of 300±20 grams were selected. Type 1 diabetes (blood sugar above 300) was induced to rats through intraperitoneal injection of streptozotocin (with a dose of 50 mg per kilogram of body weight) solution. The rats were randomly divided into two groups, diabetic training and diabetic control; The training group performed MIIT for four weeks at an intensity of 55-75% of maximum speed. Data analysis was done through independent t-test in GraphPad Prism software version 9.5. The significance level of the current research is P≤ 0.05.
Results: The intracellular content of JNK protein after four weeks of MIIT showed a significant decrease in both total (P= 0.01) and phosphorylated (P= 0.0001) forms. On the other hand, the ratio of total to phosphorylated intracellular content of JNK protein did not show a significant change after four weeks of MIIT (P= 0.23).
Conclusion: MIIT led to a decrease in the content of total and phosphorylated forms of JNK protein, which could possibly lead to less cell death in cardiac cells of diabetic subjects.
Farideh Moradi, Neda Aghaei Bahmanbeglou, Saeedeh Shadmehri, Habib Asgharpour,
Volume 24, Issue 3 (7-2024)
Abstract
Background: Diabetes can cause serious cardiovascular complications by disrupting the autophagy pathway. Therefore, this study aimed to investigate the effect of high-intensity interval training (HIIT) on the intracellular levels of autophagy proteins in the left ventricular tissue of rats with type 1 diabetes.
Methods: In this experimental study, 18 2-month-old male Sprague-Dawley rats with an average weight of 300±20 grams were selected. Twelve rats had type 1 diabetes after intraperitoneal injection of STZ (with a dose of 50 mg/kg of body weight) solution. Rats were randomly divided into two groups: diabetic training and diabetic control (each group, six heads). A healthy control group (six heads) was also considered. The training group underwent HIIT four days a week for six weeks. GraphPad Prism version 9.5 software and one-way ANOVA, and Tukey's post hoc tests were used to analyze the data. The significance level was considered P≤ 0.05.
Results: ULK1 and FIP200 levels showed a significant increase in the left ventricle after 6 weeks of HIIT training compared to the healthy control group and the diabetic control group (P= 0.0001).
Conclusion: Considering the increase in ULK1 and FIP200 proteins, it can be concluded that HIIT training can activate the autophagy pathway; Therefore, in prescribing this type of exercise for diabetic subjects, the intensity and duration of the exercise should be considered.
Maryamosadat Miri, Hamed Alizadeh Pahlavani, Mohadese Hozouri,
Volume 24, Issue 5 (12-2023)
Abstract
Background: Type 1 diabetes is characterized by persistent hyperglycemia and leads to impaired protein synthesis and ultimately muscle breakdown and reduction in muscle function. Therefore, this research was conducted with the aim of investigating the effect of moderate intensity continuous training (MICT) on the amount of 4EBP1 in the biceps muscle of type 1 diabetic rats.
Methods: The present study is of experimental-fundamental type in which 12 2-month-old male Sprague Dawley rats with an average weight of 280±30 grams participated. To induce type 1 diabetes, streptozotocin (STZ) solution was injected intraperitoneally at a dose of 65 mg/kg. 3 days after injection, blood sugar above 300 mg/dl was considered as an indicator of type 1 diabetes. After the induction of diabetes, the rats were randomly divided into 2 diabetic training groups (6 heads) and diabetic control groups (6 heads). The continuous training program (32 minutes with an intensity of 50-70% of maximum speed) was 8 weeks and 3 sessions every week. Data analysis was done through independent t-tests. Data analysis was done using GraphPad Prism software version 10.2.2. The significance level of the present study was considered P≤0.05.
Results: In the training group after 8 weeks of MICT, the intracellular content of phosphorylated (P=0.0001), total (P=0.0001) and the ratio of phosphorylated to total (P=0.002) protein 4EBP1 showed a significant change compared to the control group in the twin muscle tissue.
Conclusion: 4EBP1 protein seems to increase through 8 weeks of MICT and this mechanism can increase muscle synthesis in muscle tissue.
