Showing 23 results for Polymorphism
Sahar Shafiee, Abbasali Raz, Neda Adibi, Masoomeh Mansouri, Zohreh Annabestani, Zahra Mirzaeezadeh, Mehrdad Hashemi, Kobra Omidfar,
Volume 17, Issue 1 (3-2018)
Abstract
Background: Type 2 diabetes is the most common type of diabetes, and a complex endocrine metabolic disorder that one of its main complications is diabetic foot ulcers. Matrix metalloproteinases (MMPs) are one of the key enzymes in the reconstruction of extracellular matrix which have proteolytic activity. The aim of this research is based on evaluating relationship between -1562 C>T allele at MMP-9 gene promoter with diabetic foot ulcer in type II diabetic patients. If such correlation proves, it can be used as a prognostic biomarker in patients with high-risk.
Methods: This is a case-control study. The single nucleotide polymorphism of -1562C>T allele of MMP9 gene promoter was genotyped by hit Tetra ARMS PCR technique in 100 diabetic patients with foot ulcer grade 1 or 2 as the case group according Wagner classification and in 100 diabetic patients without foot ulcer as the control group. Results: The Chi-square test revealed significant difference in genotype frequency of CC, CT and TT alleles of -1562C>T allele of MMP9 gene promoter between case and control groups (P=0.000).
Conclusion: According to this study, there is a relationship between -1562C>T allele of MMP9 gene promoter with diabetic foot ulcer in type2 diabetes patients. Thus we can introduce this biomarker for evaluation of risk and prognosis of diabetic foot ulcers.
Fariba Soltanzadeh, Masoumeh Nezhadali, Leila Pishkar,
Volume 23, Issue 3 (9-2023)
Abstract
Background: Type 2 diabetes is a metabolic disease and the most common endocrine disorder. Visfatin is a cytokine with insulin-mimetic effects which is associated with diabetes. The rs61330082 polymorphism is located in the promoter region of visfatin gene and regulates visfatin gene expression. The aim of this study is to investigate the association of rs61330082 polymorphism of the visfatin gene with biochemical and anthropometric variables, visfatin level, diabetes and insulin resistance.
Methods: This case‑control study was performed on 80 patients with type 2 diabetes and 80 controls (persons without diabetes). Biochemical and anthropometric parameters were determined using standard methods. Insulin and visfatin levels were measured by ELISA method. Genotype was determined by polymerase chain reaction-restriction fragment length polymorphism method. The data was analyzed with SPSS software.
Results: LDL-C level in healthy people and fasting blood sugar level in patients showed a significant difference in carriers of rs61330082 polymorphism genotypes. No associations was found between visfatin level and single nucleotide polymorphism rs61330082 in the current study. Regression analysis showed that genotypes of rs61330082 polymorphism are not significantly associated with type 2 diabetes and insulin resistance.
Conclusion: The rs61330082 polymorphism is associated with LDL-C in healthy subjects and FBS in patients with T2D. Visfatin rs61330082 polymorphism is not associated with visfatin level and type 2 diabetes and insulin resistance.
Mohammad Zahmatkesh, Hosseinali Sasan, Fateme Sefid , Mohammad Yahya Vahidi Mehrjardi,
Volume 23, Issue 5 (12-2023)
Abstract
Background: Type 2 diabetes is a common multifactorial disease that has been studied by researchers in this century. These studies show the importance and position of genetics in relation to diabetes. The purpose of this study is to investigate the prevalence of rs5219 and rs5215 polymorphisms in the familial type 2 diabetic population of Yazd province.
Methods: This study is a case-control study that was conducted on 200 people (100 type 2 diabetes patients with a positive family history of type 2 diabetes and 100 healthy people without a family history of diabetes). In this study, using the ARMS-PCR technique, the genotype of the patient group and the control group were examined, and dispersion indices and parametric statistical tests such as P-value and Chi-square were used to determine the significant relationship between diabetes and genetics.
Results: Statistical analysis showed that there is a significant difference between the allelic and genotypic frequencies in both patient and control groups in rs5215 polymorphism with P=0.0015 and in rs5219 polymorphism with P=0.0342.
Conclusion: The present study showed that rs5215 and rs5219 polymorphism of KCNJ11 gene can be related to type 2 diabetes in the population of Yazd province, although extensive studies are needed to confirm these results.
