Neda Aghaei, Mohammad Sherafati Moghadam, Farhad Daryanoosh, Saeedeh Shadmehri, Shiva Jahani Golbar,
Volume 18, Issue 3 (3-2019)
Abstract
Background: The mTORC1 pathway is one of the important pathways for protein synthesis in the heart, which can lead to physiological or pathological hypertrophy. Diabetes can lead to defects in this pathway. The aim of this study was to examine the effect of 4 weeks’ aerobic training on the content of mTORC1 signaling pathway proteins in heart tissue of type 1 diabetes rats.
Methods: In this experimental study, 16 Sprague-Dawley male rats (mean weight of 300 ± 20 gr) were selected and after induction of diabetes by STZ was randomly assigned into two groups: diabetic training and diabetic control. The experimental group performed HIIT training for 4 weeks’ accordance with the training program (each session 42 minutes, 10-20 m/m) for 4 weeks, while the control group did not have any training program. Dependent t-test and independent T-test were used to analyze the data
Results: Significant increase was observed in the content of AKT1 (p<0.015), mTOR (p<0.001), P70S6K1 (p<0.006), 4EBP1 (p<0.05) proteins in the aerobic training group compared to control group.
Conclusion: Aerobic training for 4 weeks enabled to activate the pathway AKT1/mTOR/P70S6K1 and AKT1/mTOR/4E-BP1 in mTORC1 pathway; therefore, due to cardiac complications in type 1 diabetic patients, aerobic training can lead to protein synthesis and physiological cardiac hypertrophy through mTORC1 pathway.
Maryamosadat Miri, Hamed Alizadeh Pahlavani, Mohadese Hozouri,
Volume 24, Issue 5 (12-2023)
Abstract
Background: Type 1 diabetes is characterized by persistent hyperglycemia and leads to impaired protein synthesis and ultimately muscle breakdown and reduction in muscle function. Therefore, this research was conducted with the aim of investigating the effect of moderate intensity continuous training (MICT) on the amount of 4EBP1 in the biceps muscle of type 1 diabetic rats.
Methods: The present study is of experimental-fundamental type in which 12 2-month-old male Sprague Dawley rats with an average weight of 280±30 grams participated. To induce type 1 diabetes, streptozotocin (STZ) solution was injected intraperitoneally at a dose of 65 mg/kg. 3 days after injection, blood sugar above 300 mg/dl was considered as an indicator of type 1 diabetes. After the induction of diabetes, the rats were randomly divided into 2 diabetic training groups (6 heads) and diabetic control groups (6 heads). The continuous training program (32 minutes with an intensity of 50-70% of maximum speed) was 8 weeks and 3 sessions every week. Data analysis was done through independent t-tests. Data analysis was done using GraphPad Prism software version 10.2.2. The significance level of the present study was considered P≤0.05.
Results: In the training group after 8 weeks of MICT, the intracellular content of phosphorylated (P=0.0001), total (P=0.0001) and the ratio of phosphorylated to total (P=0.002) protein 4EBP1 showed a significant change compared to the control group in the twin muscle tissue.
Conclusion: 4EBP1 protein seems to increase through 8 weeks of MICT and this mechanism can increase muscle synthesis in muscle tissue.
