Showing 7 results for Atherosclerosis
Ebrahim Javadi, Alireza Shafaei, Ardavan Irvani,
Volume 2, Issue 2 (6-2003)
Abstract
Background: LDL oxidation plays a significant role in atherosclerosis process. Change in LDL type, occurring with physical activity, and the resulting decrease in LDL sensitivity to the oxidative process can protect the subject against atherosclerosis. This study has compared LDL sensitivity to oxidation in athletes and non athletes.
Methods: 14 male athletes (aged 482) and 14 male non athletes (aged 444) were recruited. 10 ml venous fasting blood was obtained from each subject. According to the PUHL guidelines, LDL sensitivity to oxidation was evaluated using conjugated diene method. Cholesterol and triglyceride were measured by enzymatic immunoassay. HDL-c was measured by precipitation method. LDL was calculated by Fried-Wald formula.
Results: LDLs from athletes’ sera were less vulnerable to oxidation than non-athletes’ sera. To say, mean lag time in athletes was 60.35 10 compared to 5610 in non athletes. This difference, however, was not statistically significant. A significant difference existed between the two groups with regard to HD L-c level (46.28 vs. 388.5 P
Seddigheh Asgari, Gholamali Naderi, Mojgan Gharipour, Gholamreza Dashti , Ali Sajadian,
Volume 3, Issue 1 (5-2004)
Abstract
New studies have introduced atherosclerosis as an inflammatory disorder. Usage of anti-inflammatory drugs thus can be useful in preventing atherosclerosis and anti-inflammatory herbal medicines without side effects seem desirable in this view.
Amirkahiria Odorutissima (AO) (umbelliferae) is an Iranian flora being traditionally used a:> an antiinflammatory drug. This study assessed the effects of this plant on the development and progression of fatty streaks.
Methods: Twenty male rabbits were obtained from Iran Pasture institute and were kept under standard condition for two weeks, then randomly divided into four groups with different diets. Groups 1 were fed on rabbit chow (normal cholesterol), group 2 were fed on a high cholesterol diet. group 3 were fed on normal cholesterol diet supplemented with AO, group 4 were fed on high cholesterol diet supplemented with AO. After 12 weeks, the animals were killed and biopsied for their aortas and right and left branches of coronary arteries. Biochemical factors were measured at baseline and after 12 weeks. Levels of cholesterol, LDL, HDL. triglyceride and fasting blood sugar (FBS) were determined by enzymatic methods, and quantitative CRP were determined with turbidometrie methods, malondialdehyde (MDA) and antioxidant capacity were determined with spectrophotometry.
Results: The results indicated that there was a significant difference between AO supplemented group compared with other groups (p<0.05) in terms of mean grade o"' fatty streak o"' right and left coronary arteries and aorta. At the end of the 12 weeks period, cholesterol. LDL and CRP were significantly reduced in the groups 3 and 4 (p<0.05).
Conclusion: This study suggests a protective role for Amirkahiria odoratissima Mozaffahan against the development of fatty streak the mechanism of which should be investigated in future studies.
Mitra Neiafar, Fatemeh Esfahanian, Alireza Esteghamati, Ramin Heshmat, Mehdi Hedayati, Masoumeh Karami, Mehrshad Abbasi, Manochehr Nakhjavani,
Volume 4, Issue 4 (6-2005)
Abstract
Background: Oxidized low-density lipoprotein (Ox-LDL), a key factor in the development of atherosclerosis, can cause endothelial dysfunction and augment lipid accumulation within the arterial wall. Increased oxidative stress in diabetes contributes to this process. Ox-LDL is a highly immunogenic molecule and it is not clear whether anti oxidized LDL antibodies (OLAB) are pathogenic or protective in atherosclerosis? The aim of this study was to evaluate Ox-LDL and its antibody in type 2 diabetes and healthy subjects.
Methods: As a case-control study we evaluated 81 type 2 diabetic patients and 69 non-diabetic healthy persons aged 40 to 65 years. Controls were sex and BMI matched with diabetic patients. Patients with history of cigarette smoking, antioxidant or antihyperlipidemic drugs consumption, coronary heart disease, hypertension , and renal impairment were excluded. We measured serum level of Ox-LDL(two monoclonal antibody of Mercodia co.) and OLAB by ELISA. Lipid profile, serum electrolytes, and HbA1c (HPLC) were also determined. Ox-LDL and its antibody were compared between diabetic patients and controls and the correlation with lipid profile, HbA1c and BMI were assessed.
Results: Serum Ox-LDL concentration and Ox-LDL to LDL ratio were distinctively higher in controls (15.7+-6.9 vs. 11.8+-5.6, P < 0.005). Ox-LDL concentrations were correlated with LDL-C (rs=0.36, P<0.0005) and total cholesterol (rs=0.31, P<0.0005) in both groups but not with age and HbA1c. In diabetic patients, Ox-LDL and its antibody were positively correlated (rs=0.26, P<0.05). Obese diabetic patients (BMI > 30) had higher Ox-LDL concentrations in comparison with diabetic patients with BMI less than 30.
Conclusion: In diabetic patients Ox-LDL level is lower than non-diabetics and is correlated with its antibodies. Based on previous findings, we suppose that the pattern of LDL oxidation enhances Ox-LDL recognition by macrophage via specific legends. This results in low serum Ox-LDL concentrations in diabetes.
Sedigheh Asgari, Alireza Ghandi, Shahriar Adibi, Gholamreza Dashti, Gholam Ali Naderi, Alireza Helalat, Narges Jafari Dinani,
Volume 6, Issue 3 (5-2007)
Abstract
Background: Atherosclerosis is the leading cause of death necessitates more research to find better solutions to this general concern. Due to the side effects of chemical compounds, more attention has been paid to herbal remedies. This study was designed to investigate the effects of Nigella sativa, on atherosclerosis and its risk factors in hypercholesterolemic rabbits.
Methods: Fifteen rabbits were randomly distributed into three groups of five each.( Normal diet group, hypercholesterolemic diet group (1% cholesterol) and group that received hypercholesterolemic diet supplemented with 5% nigella sativa). Animal groups received their own diets and water for a period of 8 weeks. At the beginning of the treatment period, blood samples were taken from all animals to measure biochemical factors. Same procedure was performed at the end of the period then the left and right coronary arteries and the aorta of each animal were dissected to study fatty streak formation (FSF).
Results: Dietary use of Nigella sativa significantly decreased cardiovascular FSF as well as blood cholesterol and LDL in hypercholesterolemic animals. Though not significant but blood HDL was increased and reduction in C-reactive protein (CRP), triglyceride (TG) and oxidized LDL was observed in the same animals.
Conclusion: The results indicate that Nigella sativa inhibits the development of atherosclerosis. This may be related to the effect of Nigella sativa on plasma lipoproteins in addition to its antioxidant and anti-inflammatory properties.
Gholamabbas Mohammady, Maryam Darabi-Amin, Mohammad Javad Sabet-Jahromi1, Reza Malek Puor -Afshar, Hassan Sheibani, Mohammad Nasry,
Volume 6, Issue 4 (6-2007)
Abstract
Background: Atherosclerosis is the leading cause of mortality in the developed and some developing countries. Some people believe that opium abuse has beneficial effects for reducing blood lipids and atherosclerosis prevention. In this study, the effect of oral opium addiction on lipid profile and atherogenesis in normal and hypercholesterolemic rabbits was studied.
Methods: Thirty two male Newzeland White rabbits were used in this study. They were divided into four groups including control, hypercholesterolemic, addicted, and hypercholesterolemic addicted and were studied for three months. The blood samples were obtained and lipid profile was determined at the beginning of the study and at the end of every month thereafter. After 90 days aorta was removed to assess for lesion formation.
Results: The levels of cholesterol, triglyceride (TG), high density lipoprotein-cholesterol (HDL-C), low density lipoprotein-cholesterol (LDL-C), and atherogenic index (total cholesterol/HDL-C) in the hypercholesterolemic and hypercholesterolemic addicted rabbits were increased significantly (P<0.001). These increases in lipids and aorta lesion areas were higher in hypercholesterolemic addicted group than hypercholesterolemic group.
Conclusion: These findings suggest that oral opium abuse affects cholesterol metabolism and depending on the dietary condition can have an aggravating effect on atherosclerosis. The protective effect of morphine on cardiac disease is not probably through the modulation of lipid metabolism.
Sedigheh Asgari, Zahra Fattahi, Gholam Ali Naderi, Shirin Azam Panah,
Volume 6, Issue 4 (6-2007)
Abstract
Background: Several studies were pointed to oxidized LDL (OX-LDL) as one of the main immunogenic agents which have important roles in primary lesions of atherosclerosis. In this study, via immunization against OX-LDL with two different antigens in an animal model (rabbit) we tried to clear relation between immune system and atherosclerosis.
Methods: LDL was isolated from healthy human plasma and oxidized with MDA or Cu++. Rabbits were divided to three groups and after 2 weeks (under basic diet) immunized with MDA-LDL or Cu-LDL. In control group Phosphate-buffered saline (PBS) was used. Immunization was repeated with these materials again the weeks of 2, 4, 6, and 8 and concentration of OX-LDL antibody was measured in each stage. At the end of 8th week, rabbits fed normal or high cholesterol regimens. Biochemical factors were measured at the beginning and end of study, also Fatty streaks in aorta and left and right coronary arteries was evaluated.
Results: Immunization with Cu -LDL and MDA-LDL induced adequate antibody formation (IgG) at the end of 8th weeks. Immunization with MDA-LDL significantly decreased the level of cholesterol, LDL-cholesterol and fasting blood sugar (FBS) (P= 0.04). Also a significant decrease in fatty streak lesions was detected in aorta and right and left coronary arteries as compared with non-immunized high-cholesterol group (P= 0.04). Immunization with Cu -LDL significantly decreased Triglyceride, FBS and cholesterol as compared with non-immunized high cholesterol. No differences were detected in the fatty streak lesions in this group as compared with non-immunized high-cholesterol diet cu+2-LDL immunized group, shown significant decrease in CRP as compared with both non-immunized group and high cholesterol diet.
Conclusion: We conclude the protective effects of immune responses on atherosclerosis depend to what kind of antibody, so MDA-LDL and CU-LDL prevent atherosclerosis with different mechanism.
Safdar Mahdavifard, Seyede Zahra Bathaie, Manouchehr Nakhjavani , Batoul Etemadi Kia,
Volume 13, Issue 4 (5-2014)
Abstract
Background: Diabetes is the most common metabolic diseases and its vascular complications are main cause of death in diabetic patients. Patients with hyperglycemia, dyslipidemia and oxidative stress are prone to diabetes complications. The goal of this study was investigation of the effect of cysteine (Cys) on hyperglycemia, lipid profile, atherogenic index, glyoxal, methylglyoxal, oxidative stress and, glycation and oxidation of LDL in the rat model of diabetes –atherosclerosis.
Methods: Diabetes was induced in the rats using Streptozotocin injection then they put on the atherogenic diet. The groups under study were including of control and diabetic rats, and two other similar groups under Cys (0.05 % in dirking water) treatment. After one month, fasting blood sugar (FBS), lipid profile, atherogenic index (LDL/HDL), glycated and oxidized LDL, AGEs, glyoxal, methylglyoxal, Advanced oxidation protein products (AOPP) as an oxidative stress index and weight of rat was measured.
Results: Diabetic-atherosclerotic rat groups significantly showed higher level of FBS, triglyceride, cholesterol, LDL, atherogenic index, glycated and oxidized LDL, glyoxal, methylglyoxal and AOPP than control group. These parameters significantly (P < 0.001) reduced in diabetic group treated with Cys in comparison of untreated.
Conclusion: Cysteine with improving property on glycemic and lipemic conditions, inhibitory activity on glycation and oxidation of LDL and reduction of oxidative stress in diabetic-atherosclerotic rats could recommended as a drug for prevention of diabetes complications.
