Homna Abdi, Eidi Alijani, Mahsa Mohsen Zadeh,
Volume 20, Issue 2 (1-2021)
Abstract
Background: Although some studies have studied the mechanism of action of beta cells in animal models and more or less in human populations, but so far the role of exercise therapy or exercise exercise HIIT with black grape supplementation on the expression of genes involved in pancreatic beta cells This study investigates the effect of black grape supplementation combined with intense intermittent exercise on Bcl-2 and Bax genes in pancreatic tissue of rats with type 2 diabetes.
Methods: The study was conducted in the form of an experimental design. The subjects of this project were 40 8-month-old male rats with an average weight of 250 grams. After familiarizing the subjects with exercise and induction of diabetes by STZ, the subjects were randomly divided into 5 groups including exercise, supplement, exercise and supplement, diabetic control and basic control. After 8 weeks of training, which was 5 sessions per week for 8 weeks, the activity was on a treadmill with an intensity of 90% Vo2max and supplementation with black grape seed extract. Bcl-2 and Bax genes were measured after RNA extraction from pancreas and cDNA synthesis. Bcl-2 and Bax genes were measured by Time-Real PCR. Data were analyzed using independent t-test and two-factor analysis of variance at the significance level of 0.05 by SPSS software version 24.
Results: The results showed that the main effect of exercise had no significant effect on Bcl-2 gene expression. The main effect of the extract on the expression of this gene was significant. Also, the interaction between exercise and Bcl-2 supplementation was not significant. Regarding Bax gene expression, it was shown that the main effect of exercise on the gene was significant. The main effect of extract and interaction of exercise and supplementation on Bax gene expression was not significant. No significant changes were observed in the effect of exercise and extract on the expression of Bcl-2 and Bax genes.
Conclusion: It seems that regulating the expression of Bcl-2 and Bax genes through exercise and consumption of black grape seed extract is likely to improve and maintain pancreatic beta cell function in diabetic rats.
Masomeh Sarmadiyan, Eidy Aliijani, Fuad Feizolahi, Davood Khorshidi,
Volume 22, Issue 3 (9-2022)
Abstract
Background: Type 1 diabetes is a disorder caused by autoimmune destruction of pancreatic insulin-producing cells. This induction of autoimmunity may be due to genetic and environmental factors. Bax and Bcl2 proteins play an important role in the process of apoptosis.
Methods: In this study, 30 male Wistar rats weighting approximately 200±20gr were randomly selected from available rats in lab (500). Subjects after 2 weeks of familiarity with the environment were randomly divided into 5 groups of 6, including (diabetes + injection, exercise) and (diabetes + exercise) and (diabetes + injection) and (diabetes control to control the passage of time) and (basic diabetes to Defaults) under the same laboratory conditions and developed type 1 diabetes by intraperitoneal injection of streptozotocin (stz) (60 mg / kg). Rats in the diabetic group and the diabetic group + stem cell injection had a total of 17 sessions of resistance training for 5 weeks.
Results: The results of the present study showed that there was no significant difference between the mean of Bax and Bcl2 in the resistance training group with simultaneous injection of stem cells and the training group.
Conclusion: The results of our study showed that performing 17 sessions of resistance training alone with stem cell injection was effective on the expression of Bax protein and Bcl2, but there was no significant difference between the effect of training alone and training with stem cells.
