Showing 3 results for Black Grape Seed Extract
Fatemeh Soltanmohammadi, Mahsa Mohsenzadeh, Fuad Feizollahi,
Volume 19, Issue 2 (1-2020)
Abstract
Background: The aim of the present study was to investigate the effect of 8 weeks of HIIT training and supplementation of black grape seed extract on Wnt and Β-catenin gene expression in pancreatic tissue in male rats with type 2 diabetes.
Methods: In this experimental study, 40 male Wistar rats, with average weight of 250 ± 20 gr, after induction of diabetes by STZ, were randomly divided into 5 groups: training- supplement, training-placebo, supplement and diabetic-control and base control. After becoming diabetic, rats were familiarized with protocol for one week and then trained for eight weeks, five days a week. They ran on treadmill with 90% of VO2 max. Intragastric injection of GSPE extracted from black grape seed (Cabernet sauvignon) once a day for 8 weeks (250 mg/kg, proanthocyanidin content >96%). 24 hours after the last session of training, pancreas tissue was extracted, and the level of gene expression was measured by Real Time-PCR. In all statistical analyzes, the statistical significance level was considered to be 0.05.
Results: Eight weeks and supplement significantly increased the mean expression of Wnt and Β-catenin genes in pancreatic tissue of type 2 diabetic rats but the effect of exercise with black grape seed extract on the expression of Wnt genes in the pancreas of diabetic rats was not significant.
Conclusion: It seems that regulating the expression of Wnt and Β-catenin genes through exercise and consumption of black grape seed extract is likely to improve and maintain the function of pancreatic beta cells, especially in diabetic samples.
Bahare Ghani, Mahsa Mohsenzadeh, Foad Feyzollahi,
Volume 19, Issue 5 (6-2020)
Abstract
Backgrond: Although some studies have studied the mechanism of action of beta cells in animal models and more or less in human populations, but so far the role of exercise therapy or exercise exercise HIIT along with black grape supplementation on the expression of involved genes Less has been studied in pancreatic beta cells, so the aim of the present study was to investigate the effect of eight weeks of intense intermittent exercise with black grape supplementation on the expression of PPARα and PPARγ gene in pancreatic tissue of male rats with type 2 diabetes.
Methods: The study was conducted in the form of an experimental design. The subjects of this project were 40 8-month-old male rats with an average weight of 250 grams. After familiarizing the subjects with exercise and induction of diabetes by STZ, the subjects were randomly divided into 5 groups including exercise, supplement, exercise and supplement, diabetic control and basic control. After 8 weeks of training, 5 sessions per week for 8 weeks with 90% Vo2max activity and supplementation of black grape seed extract, PPARα and PPARγ genes were measured after RNA extraction from pancreas and cDNA synthesis. PPARα and PPARγ genes were measured by Time-Real PCR. Data were analyzed using independent t-test and two-factor analysis of variance at a significance level of 0.05 by SPSS software version 24.
Results: The effect of exercise with consumption of black grape seed extract on the expression of PPARγ and PPARα genes in the pancreas of diabetic rats was not significant, but exercise alone significantly increased the expression of PPARα gene in the pancreatic tissue of diabetic specimens. Consumption of black grape seed extract alone significantly increased the expression of PPARγ and PPARα genes.
Conclusion: It seems that regulating the expression of PPARα and PPARγ genes through exercise and consumption of black grape seed extract may lead to the improvement and maintenance of pancreatic beta cell function in diabetic rats.
Homna Abdi, Eidi Alijani, Mahsa Mohsen Zadeh,
Volume 20, Issue 2 (1-2021)
Abstract
Background: Although some studies have studied the mechanism of action of beta cells in animal models and more or less in human populations, but so far the role of exercise therapy or exercise exercise HIIT with black grape supplementation on the expression of genes involved in pancreatic beta cells This study investigates the effect of black grape supplementation combined with intense intermittent exercise on Bcl-2 and Bax genes in pancreatic tissue of rats with type 2 diabetes.
Methods: The study was conducted in the form of an experimental design. The subjects of this project were 40 8-month-old male rats with an average weight of 250 grams. After familiarizing the subjects with exercise and induction of diabetes by STZ, the subjects were randomly divided into 5 groups including exercise, supplement, exercise and supplement, diabetic control and basic control. After 8 weeks of training, which was 5 sessions per week for 8 weeks, the activity was on a treadmill with an intensity of 90% Vo2max and supplementation with black grape seed extract. Bcl-2 and Bax genes were measured after RNA extraction from pancreas and cDNA synthesis. Bcl-2 and Bax genes were measured by Time-Real PCR. Data were analyzed using independent t-test and two-factor analysis of variance at the significance level of 0.05 by SPSS software version 24.
Results: The results showed that the main effect of exercise had no significant effect on Bcl-2 gene expression. The main effect of the extract on the expression of this gene was significant. Also, the interaction between exercise and Bcl-2 supplementation was not significant. Regarding Bax gene expression, it was shown that the main effect of exercise on the gene was significant. The main effect of extract and interaction of exercise and supplementation on Bax gene expression was not significant. No significant changes were observed in the effect of exercise and extract on the expression of Bcl-2 and Bax genes.
Conclusion: It seems that regulating the expression of Bcl-2 and Bax genes through exercise and consumption of black grape seed extract is likely to improve and maintain pancreatic beta cell function in diabetic rats.
