Showing 4 results for Caffeine
Maryam Seyed Heaydari, Abbas Aadeghi, Ali Hemati Afif,
Volume 19, Issue 2 (1-2020)
Abstract
Background: Cytokines are immune related factors. Production of pro inflammatory cytokines in diabetic patients is increased, which is exacerbated by vigorous training. This study investigates the response of serum IL-2 and IL-4 to eight-week high Intensity Interval Training (HIIT) with caffeine intake on inflammatory and anti-inflammatory responses in diabetic rats.
Methods: In a clinical-interventional animal study, 50 male rats were randomly assigned to 5 equal groups (control group (C), diabetes group (D), diabetes + caffeine group (D+Ca), diabetes + training group (D+T), diabetes + training + caffeine group (D+Ca+T)) and subjected to 8 weeks of caffeine supplementation (70 mg / kg of caffeine powder was injected five days each week) and 8 weeks of 5 sessions per week with 6 to 12 times, 2-min intervals with intensity of 85-90% of maximal speed. 48 hours after the last training session, immunological factors including IL-2 and IL-4 were measured. Statistical analysis of variables was performed using One-way ANOVA and Tukey tests at the significant level (P<0.05).
Results: The results showed that (D+Ca) group had the lowest IL2 in comparison with (D) group (P= 0.001). IL-4 had the highest in (D+Ca) and (D+Ca+T) groups (P = 0.001). But these differences were not statistically significant between (D+Ca) and (D+Ca+T) groups (P= 0.67).
Conclusion: It seems that caffeine to play a role in improving the status of IL-2 and IL-4 in streptozotocin-induced diabetes, but HIIT exercises are not effective in this regard.
Afshar Jafari, Ali Zarghami Khameneh, Saeid Nikookheslat, Pooran Karimi,
Volume 19, Issue 4 (4-2020)
Abstract
Background: Autophagy is a new therapeutic strategy aimed at reducing the diabetic abnormalities. While excessive or insufficient autophagic activity during diabetes leads to altered cellular homeostasis. So, aim of the present study was conducted to determine the effect of eight-week high-intensity interval training (HIIT) along with caffeine injection on the levels of some myocardial autophagy-related proteins in diabetic rats.
Methods: In experimental design, fifty male white wistar rats with an age range of 3-2 months (average weight 250±25 g) were randomly divided into 5 groups of homogeneous 10 rats in each group: Healthy control (C: intraperitoneal injection of saline), Diabetic control (D: high-fat diet combined with a single intraperitoneal injection of streptozotocin, Diabetic with training (D+T: running with intensity at the 85-90% of maximum speed in 5 to 12 bout of 2 min-1; 5 days/week for 8 weeks), Diabetic with caffeine supplementation(D+CA: intraperitoneal injection of pure caffeine at 70 mg.kg-1 5 days/week for 8 weeks), Diabetic with training and with caffeine supplementation (D+T+CA). For evaluate changes in the expression profile of some of the genes associated with autophagy signaling pathway (LC3-II, ULK-1, Beclin1) in the myocardium (left ventricular), based on Western blot analysis will be used. Also, the one-way analysis of variance (ANOVA) and Tukey post hoc test were be used to analyze the data.
Results: The expression of all autophagic proteins in diabetic with trained and non-trained groups was higher than in healthy
group (P≤0.05). On the one hand, the expression of autophagy-related proteins in the trained group with caffeine supplementation was significantly higher than that of the training group without caffeine intake (P=0.001).
Conclusion: The findings of this study suggest that caffeine injection exacerbated the expression of autophagic proteins induced by diabetes; On the other hand, high-intensity interval training can as a preventive strategy, modulate diabetes-induced myocardial autophagy.
Morteza Khalili, Abbas Sadeghi, Mohammad Javad Javad Maleki,
Volume 19, Issue 5 (6-2020)
Abstract
Background: The relationship between low PGC-1α expression and several metabolic diseases such as diabetes and obesity has been identified. This study investigates the effect of eight-week high intensity interval training (HIIT) and caffeine intake on mitochondrial biogenesis in soleus muscle in diabetic rats induced Streptozotocin.
Methods: In a clinical-interventional animal study, 50 male rats were randomly assigned to 5 equal groups (control group(C), diabetes group(D), diabetes + caffeine group(D+Caf), diabetes + training group(D+T), diabetes + training + caffeine group(D+CAF+T)) and subjected to 8 weeks of caffeine supplementation (70 mg / kg of caffeine powder was injected five days each week) and 8 weeks of 5 sessions per week with 6 to 12 times, 2-min intervals with intensity of 85-90% of maximal speed. Blood was collected directly from the left ventricle to measure blood glucose levels. The soleus muscle of the left leg was extracted and PGC-1α measured by Western Blot method. Independent t-tests, two-way analysis of variance, and Eta squared (p <0.05) were used to analyze the data.
Results: The results showed that induction of diabetes significantly increased blood glucose (P <0.01) and significantly decreased mRNAPGC-1α (P = 0.002). Also, both high-intensity interval training (p = 0.001) and caffeine supplementation (p = 0.03) significantly increased mRNAPGC-1α.
Conclusion: Based on the results of this study, it is possible to suggest the use of HIIT and caffeine consumption as an effective intervention in increasing mitochondrial biogenesis in diabetics. However, a clear statement in this regard requires further research in this area.
Bahram Jamali Gharakhanlou, Akram Ameghani, Ali Zarghami Khameneh,
Volume 21, Issue 3 (9-2021)
Abstract
Background: Imbalanced production of adipokines as leptin and adiponectin in diabetic patients may lead to the development of metabolic complications. Therefore, the aim of this study was to determine the effects of 2-month of caffeine ingestion along with aerobic training on changes in serum leptin and adiponectin levels and leptin/adiponectin ratio (LAR) in type 2 diabetic men.
Methods: Thirty-two diabetic men participated in
a quasi-experimental designs in the four groups for two-months period of a aerobic training (treadmill walking 3 times/week, 1.5 hour/session, 65-85% HRR) with and without caffeine ingestion (3 mg.kg
-1.day). Serum changes in leptin and adiponectin were measured during two phases (baseline and 24-hours after completing of the training program). Data were analyzed by one-way ANOVA and bonferroni's post-hoc test at level P≤0.05.
Results: Administration of two-months caffeine (CA) alone and combination with aerobic training (AT) were significantly could reduced and increased in leptin and adiponectin level, respectively (P=0.001). Thus, the combined group (AT+CA) effect were far more appropriate intervention in changing the studied indices (P=0.001). Also, the LAR method was notable reduced in all study groups, although these effects were more significantly in the combined group (AT+CA) (P=0.001).
Conclusion: It seems administration of caffeine supplementation and aerobic training for two-months have a positive effects on the improved relative of leptin and adiponectin levels as well as their ratio in diabetics, although the combination of these two variables has been shown to have far more dual effects.
