Majid Kashef, Mojtaba Salehpour, Fereshteh Shahidi, Nematallh Nejatmand Malari,
Volume 23, Issue 2 (5-2023)
Abstract
Background: The main cause of death of diabetic patients is heart disease. Exercise improves insulin resistance and reduces the mortality of diabetic patients. The aim of this research was the effect of six weeks endurance training on some miRs related to diabetic cardiomyopathy, FBS and insulin resistance index in male Wistar rats.
Methods: After induction of diabetes, male Wistar rats with an average weight of 200gr and age of 8 weeks were randomly classified into four groups: sham, control, endurance training and healthy. Endurance training was performed for 6 weeks. Gene expression was investigated using Real Time PCR. The statistical tests of this research were One Way Anova and T Correlated. The data were normalized by GapDh method and 2-(∆∆CT).
Results: Endurance training leads to a significant decrease in FBS (P: 0.00) and insulin resistance index (P: 0.00) and there was a significant increase in VO2peak (P: 0.001). The expression levels of miR-373 and miR-195 in the exercise group did not change significantly compared to the diabetic control group (P > 0.05). But the effect of training led to an increase in the expression of miR-373 (P: 0.4) and a decrease in the expression of miR-195 (P: 0.9).
Conclusion: Aerobic exercise improved the aerobic capacity of the exercise group. In addition, exercise led to the relative improvement of diabetes complications (increasing the expression level of miR-373 and decreasing the expression level of miR-195). Therefore, it is recommended that patients with cardiomyopathy should focus on aerobic exercises.
Reza Hassanizadeh, Khosro Jalali Dehkordi, Farzaneh Taghian,
Volume 24, Issue 6 (2-2025)
Abstract
Background: Type 2 diabetes and its relationship with cardiovascular diseases is one of the concerns of today's society. Although the favorable role of aerobic exercise and gymnema sylvester supplement consumption in diabetic patients has been reported, the effect of aerobic exercise and gymnema sylvester supplement consumption on heart tissue is not well known. Therefore, the purpose of this research is Comparison of the effect of aerobic exercise and gymnema sylvestre supplement on some markers related to cardiomyopathy in type 2 diabetic mice.
Methods: The present research was experimental. The statistical samples included 40 male C57BL/6 mice (with an average age of 8 to 10 weeks and a weight of 20 to 25 gr). who became diabetic after 20 weeks of feeding with a high-fat diet. Then randomly divided into five eight groups including: diabetes + exercise (TDM), diabetes + gymnema sylvester (GSDM), diabetes + exercise + gymnema sylvester (TGSDM), diabetes (DM) and healthy control (CN). The aerobic exercise program consisted of exercises at a speed of 10 m/min for six weeks and five training sessions per week on a treadmill. Daily consumption of gymnema sylvester at the rate of 0.1 g/kg orally alone and also with training intervention was done for six weeks. Serum glucose concentration was measured by enzymatic method, insulin level by ELISA method, insulin resistance by HOMA-IR method and expression of CASPASE-1 and NLRP3 genes by RT-PCR method. Data evaluation was calculated using covariance statistical test and Bonferroni's post hoc test, the significance level was considered as P≤ 0.05.
Results: The findings showed that aerobic exercise and gymnema sylvestre supplement alone caused a significant decrease in the levels of glucose, insulin, insulin resistance and the expression of CASPASE-1 and NLRP3 genes in the heart tissue (P≤ 0.05), the largest decrease in the dependent variables related to the aerobic exercise group with Gymnema Sylvester supplement (P≤0.05). Also, there is no significant difference between the dependent variables of the diabetes group and aerobic exercise and the diabetes group and Gymnema Sylvester supplement (P≤ 0.05).
Conclusion: Six weeks of aerobic exercise, gymnema sylvester supplement and aerobic exercise combined with gymnema sylvester supplement improves blood variables by reducing glucose, insulin and insulin resistance, as well as improving markers related to cardiomyopathy by reducing the expression of caspase-1 and NLRP3 genes in the heart tissue in type 2 diabetic mice.
